NEDD1 (NM_152905) Human Tagged ORF Clone

CAT#: RG205524

NEDD1 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 1 (NEDD1), transcript variant 2

Product Images

Circular map for RG205524

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NEDD1
• Synonyms: GCP-WD; TUBGCP7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205524 representing NM_152905
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGAAAACCTCAGATTTGCTTCATCAGGAGATGATATTAAAATATGGGATGCTTCATCTATGACAT
  TGGTGGATAAATTCAACCCACACACATCACCACATGGAATCAGCTCAATATGTTGGAGCAGCAATAATAA
  CTTTTTAGTAACAGCATCTTCCAGTGGCGACAAAATAGTTGTCTCAAGTTGCAAATGTAAACCTGTTCCA
  CTTTTAGAGCTTGCTGAAGGGCAAAAGCAGACATGTGTCAATTTAAATTCTACATCTATGTATTTGGTAA
  GCGGAGGCCTAAATAACACTGTTAATATTTGGGATTTAAAATCAAAAAGAGTTCATCGATCTCTTAAGGA
  TCATAAAGATCAAGTAACTTGTGTAACATACAATTGGAATGATTGCTACATTGCTTCTGGATCTCTTAGT
  GGTGAAATTATTTTACACAGTGTAACCACTAATTTATCTAGTACTCCTTTTGGCCATGGTAGTAACCAGT
  CTGTTCGGCACTTGAAGTACTCCTTGTTTAAGAAATCACTACTGGGCAGTGTTTCGGATAATGGAATAGT
  AACTCTCTGGGATGTAAATAGTCAGAGTCCATACCATAACTTTGACAGTGTACACAAAGCTCCAGCGTCA
  GGCATCTGTTTTTCTCCTGTCAATGAATTGCTCTTTGTAACCATAGGCTTGGATAAAAGAATCATCCTCT
  ATGACACTTCAAGTAAGAAGCTAGTGAAAACTTTAGTGGCTGACACTCCTCTAACTGCGGTAGATTTCAT
  GCCTGATGGAGCCACTTTGGCTATTGGATCTTCCCGGGGGAAAATATATCAATATGATTTAAGAATGTTG
  AAATCACCAGTTAAGACCATCAGTGCTCACAAGACATCTGTGCAGTGTATAGCATTTCAGTACTCCACTG
  TTCTTACTAAGTCAAGTTTAAATAAAGGCTGTTCAAATAAGCCCACAACAGTGAACAAACGAAGTGTTAA
  TGTGAATGCTGCTAGTGGAGGAGTTCAGAATTCCGGAATTGTCAGAGAAGCACCTGCCACGTCCATTGCC
  ACAGTTCTACCACAACCTATGACATCAGCTATGGGGAAAGGAACAGTTGCTGTTCAAGAAAAAGCAGGTT
  TGCCTCGAAGCATAAACACAGACACTTTATCTAAGGAAACAGACAGTGGAAAAAATCAGGATTTCTCCAG
  CTTTGATGATACTGGGAAAAGTAGTTTAGGTGACATGTTCTCACCTATCAGAGATGATGCTGTAGTTAAC
  AAGGGAAGTGATGAGTCCATAGGCAAAGGAGATGGCTTTGACTTTCTACCGCAGTTGAACTCAGTGTTTC
  CTCCAAGAAAAAATCCAGTAACTTCAAGTACTTCAGTATTGCATTCTAGTCCTCTTAATGTTTTTATGGG
  ATCTCCAGGGAAAGAGGAAAATGAAAACCGTGATCTAACAGCTGAGTCTAAGAAAATATATATGGGAAAA
  CAGGAATCTAAAGACTCCTTCAAACAGTTAGCAAAGTTGGTCACATCTGGTGCTGAAAGTGGAAATCTAA
  ATACCTCTCCATCATCTAACCAAACAAGAAATTCTGAGAAATTTGAAAAGCCAGAGAATGAAATTGAAGC
  CCAGTTGATATGTGAACCCCCAATCAATGGATCCTCAACTCCAAATCCAAAGATAGCATCTTCTGTCACT
  GCTGGAGTTGCCAGTTCACTCTCAGAAAAAATAGCCGACAGCATTGGAAATAACCGGCAAAATGCACCAT
  TGACTTCCATTCAAATTCGTTTTATTCAGAACATGATACAGGAAACGTTGGATGACTTTAGAGAAGCATG
  CCATAGGGACATTGTGAATTTGCAAGTGGAGATGATTAAACAGTTTCATATGCAACTGAATGAAATGCAT
  TCTTTGCTGGAAAGATACTCAGTGAATGAAGGTTTAGTGGCTGAAATTGAAAGACTACGAGAAGAAAACA
  AAAGATTACGGGCCCACTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205524 representing NM_152905
  Red=Cloning site Green=Tags(s)
  
  MQENLRFASSGDDIKIWDASSMTLVDKFNPHTSPHGISSICWSSNNNFLVTASSSGDKIVVSSCKCKPVP
  LLELAEGQKQTCVNLNSTSMYLVSGGLNNTVNIWDLKSKRVHRSLKDHKDQVTCVTYNWNDCYIASGSLS
  GEIILHSVTTNLSSTPFGHGSNQSVRHLKYSLFKKSLLGSVSDNGIVTLWDVNSQSPYHNFDSVHKAPAS
  GICFSPVNELLFVTIGLDKRIILYDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIYQYDLRML
  KSPVKTISAHKTSVQCIAFQYSTVLTKSSLNKGCSNKPTTVNKRSVNVNAASGGVQNSGIVREAPATSIA
  TVLPQPMTSAMGKGTVAVQEKAGLPRSINTDTLSKETDSGKNQDFSSFDDTGKSSLGDMFSPIRDDAVVN
  KGSDESIGKGDGFDFLPQLNSVFPPRKNPVTSSTSVLHSSPLNVFMGSPGKEENENRDLTAESKKIYMGK
  QESKDSFKQLAKLVTSGAESGNLNTSPSSNQTRNSEKFEKPENEIEAQLICEPPINGSSTPNPKIASSVT
  AGVASSLSEKIADSIGNNRQNAPLTSIQIRFIQNMIQETLDDFREACHRDIVNLQVEMIKQFHMQLNEMH
  SLLERYSVNEGLVAEIERLREENKRLRAHF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152905
• ORF Size: 1980 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152905.4
• RefSeq Size: 2374 bp
• RefSeq ORF: 1983 bp
• Locus ID: 121441
• UniProt ID: Q8NHV4
• Cytogenetics: 12q23.1
• Domains: WD40
• Gene Summary: Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.[UniProtKB/Swiss-Prot Function]