PIWIL1 (NM_004764) Human Tagged ORF Clone

CAT#: RG205469

PIWIL1 (tGFP-tagged) - Human piwi-like 1 (Drosophila) (PIWIL1), transcript variant 1

Product Images

Circular map for RG205469

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIWIL1
• Synonyms: CT80.1; HIWI; MIWI; PIWI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205469 representing NM_004764
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGGGAGAGCCCGAGCCAGAGCCAGAGGAAGGGCCCGCGGTCAGGAGACAGCGCAGCTGGTGGGCT
  CCACTGCCAGTCAGCAACCTGGTTATATTCAGCCTAGGCCTCAGCCGCCACCAGCAGAGGGGGAATTATT
  TGGCCGTGGACGGCAGAGAGGAACAGCAGGAGGAACAGCCAAGTCACAAGGACTCCAGATATCTGCTGGA
  TTTCAGGAGTTATCGTTAGCAGAGAGAGGAGGTCGTCGTAGAGATTTTCATGATCTTGGTGTGAATACAA
  GGCAGAACCTAGACCATGTTAAAGAATCAAAAACAGGTTCTTCAGGCATTATAGTAAGGTTAAGCACTAA
  CCATTTCCGGCTGACATCCCGTCCCCAGTGGGCCTTATATCAGTATCACATTGACTATAACCCACTGATG
  GAAGCCAGAAGACTCCGTTCAGCTCTTCTTTTTCAACACGAAGATCTAATTGGAAAGTGTCATGCTTTTG
  ATGGAACGATATTATTTTTACCTAAAAGACTACAGCAAAAGGTTACTGAAGTTTTTAGTAAGACCCGGAA
  TGGAGAGGATGTGAGGATAACGATCACTTTAACAAATGAACTTCCACCTACATCACCAACTTGTTTGCAG
  TTCTATAATATTATTTTCAGGAGGCTTTTGAAAATCATGAATTTGCAACAAATTGGACGAAATTATTATA
  ACCCAAATGACCCAATTGATATTCCAAGTCACAGGTTGGTGATTTGGCCTGGCTTCACTACTTCCATCCT
  TCAGTATGAAAACAGCATCATGCTCTGCACTGACGTTAGCCATAAAGTCCTTCGAAGTGAGACTGTTTTG
  GATTTCATGTTCAACTTTTATCATCAGACAGAAGAACATAAATTTCAAGAACAAGTTTCCAAAGAACTAA
  TAGGTTTAGTTGTTCTTACCAAGTATAACAATAAGACATACAGAGTGGATGATATTGACTGGGACCAGAA
  TCCCAAGAGCACCTTTAAGAAAGCCGACGGCTCTGAAGTCAGCTTCTTAGAATACTACAGGAAGCAATAC
  AACCAAGAGATCACCGACTTGAAGCAGCCTGTCTTGGTCAGCCAGCCCAAGAGAAGGCGGGGCCCTGGGG
  GGACACTGCCAGGGCCTGCCATGCTCATTCCTGAGCTCTGCTATCTTACAGGTCTAACTGATAAAATGCG
  TAATGATTTTAACGTGATGAAAGACTTAGCCGTTCATACAAGACTAACTCCAGAGCAAAGGCAGCGTGAA
  GTGGGACGACTCATTGATTACATTCATAAAAACGATAATGTTCAAAGGGAGCTTCGAGACTGGGGTTTGA
  GCTTTGATTCCAACTTACTGTCCTTCTCAGGAAGAATTTTGCAAACAGAAAAGATTCACCAAGGTGGAAA
  AACATTTGATTACAATCCACAATTTGCAGATTGGTCCAAAGAAACAAGAGGTGCACCATTAATTAGTGTT
  AATCCACTAGATAACTGGCTGTTGATCTATACGCGAAGAAATTATGAAGCAGCCAATTCATTGATACAAA
  ATCTATTTAAAGTTACACCAGCCATGGGCATGCAAATGAAAAAAGCAATAATGATTGAAGTGGATGACAG
  AACTGAAGCCTACTTAAGAGTCTTACAGCAAAAGGTCACAGCAGACACCCAGATAGTTGTCTGTCTGTTG
  TCAAGTAATCGGAAGGACAAATACGATGCTATTAAAAAATACCCGTGTACAGATTGCCCTACCCCAAGTC
  AGTGTGTGGTGGCCCGAACCTTAGGCAAACAGCAAACTGTCATGGCCATTGCTACAAAGATTGCCCTACA
  GATGAACTGCAAGATGGGAGGAGAGCTCTGGAGGGTGGACATCCCCCTGAAGCTCGTGATGATCGTTGGC
  ATCGATTGTTACCATGACATGACAGCTGGGCGGAGGTCAATCGCAGGATTTGTTGCCAGCATCAATGAAG
  GGATGACCCGCTGGTTCTCACGCTGCATATTTCAGGATAGAGGACAGGAGCTGGTAGATGGGCTCAAAGT
  CTGCCTGCAAGCGGCTCTGAGGGCTTGGAATAGCTGCAATGAGTACATGCCCAGCCGGATCATCGTGTAC
  CGCGATGGCGTAGGAGACGGCCAGCTGAAAACACTGGTGAACTACGAAGTGCCACAGTTTTTGGATTGTC
  TAAAATCCATTGGTAGAGGTTACAACCCTAGACTAACGGTAATTGTGGTGAAGAAAAGAGTGAACACCAG
  ATTTTTTGCTCAGTCTGGAGGAAGACTTCAGAATCCACTTCCTGGAACAGTTATTGATGTAGAGGTTACC
  AGACCAGAATGGTATGACTTTTTTATCGTGAGCCAGGCTGTGAGAAGTGGTAGTGTTTCTCCCACACATT
  ACAATGTCATCTATGACAACAGCGGCCTGAAGCCAGACCACATACAGCGCTTGACCTACAAGCTGTGCCA
  CATCTATTACAACTGGCCAGGTGTCATTCGTGTTCCTGCTCCTTGCCAGTACGCCCACAAGCTGGCTTTT
  CTTGTTGGCCAGAGTATTCACAGAGAGCCAAATCTGTCACTGTCAAACCGCCTTTACTACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205469 representing NM_004764
  Red=Cloning site Green=Tags(s)
  
  MTGRARARARGRARGQETAQLVGSTASQQPGYIQPRPQPPPAEGELFGRGRQRGTAGGTAKSQGLQISAG
  FQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVRLSTNHFRLTSRPQWALYQYHIDYNPLM
  EARRLRSALLFQHEDLIGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQ
  FYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRSETVL
  DFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQY
  NQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQRE
  VGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISV
  NPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMKKAIMIEVDDRTEAYLRVLQQKVTADTQIVVCLL
  SSNRKDKYDAIKKYPCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVG
  IDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVY
  RDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVT
  RPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAF
  LVGQSIHREPNLSLSNRLYYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004764
• ORF Size: 2583 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004764.2
• RefSeq Size: 3416 bp
• RefSeq ORF: 2586 bp
• Locus ID: 9271
• UniProt ID: Q96J94
• Cytogenetics: 12q24.33
• Protein Pathways: Dorso-ventral axis formation
• Gene Summary: This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]