Endothelin A Receptor (EDNRA) (NM_001957) Human Tagged ORF Clone

CAT#: RG205385

EDNRA (tGFP-tagged) - Human endothelin receptor type A (EDNRA), transcript variant 1

Product Images

Circular map for RG205385

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Endothelin A Receptor
• Synonyms: ET-A; ETA; ETA-R; ETAR; ETRA; hET-AR; MFDA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205385 representing NM_001957
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCCTTTGCCTCAGGGCATCCTTTTGGCTGGCACTGGTTGGATGTGTAATCAGTGATAATCCTG
  AGAGATACAGCACAAATCTAAGCAATCATGTGGATGATTTCACCACTTTTCGTGGCACAGAGCTCAGCTT
  CCTGGTTACCACTCATCAACCCACTAATTTGGTCCTACCCAGCAATGGCTCAATGCACAACTATTGCCCA
  CAGCAGACTAAAATTACTTCAGCTTTCAAATACATTAACACTGTGATATCTTGTACTATTTTCATCGTGG
  GAATGGTGGGGAATGCAACTCTGCTCAGGATCATTTACCAGAACAAATGTATGAGGAATGGCCACAACGC
  GCTGATAGCCAGTCTTGCCCTTGGAGACCTTATCTATGTGGTCATTGATCTCCCTATCAATGTATTTAAG
  CTGCTGGCTGGGCGCTGGCCTTTTGATCACAATGACTTTGGCGTATTTCTTTGCAAGCTGTTCCCCTTTT
  TGCAGAAGTCCTCGGTGGGGATCACCGTCCTCAACCTCTGCGCTCTTAGTGTTGACAGGTACAGAGCAGT
  TGCCTCCTGGAGTCGTGTTCAGGGAATTGGGATTCCTTTGGTAACTGCCATTGAAATTGTCTCCATCTGG
  ATCCTGTCGTTTATCCTGGCCATTCCTGAAGCGATTGGCTTCGTCATGGTACCCTTTGAATATAGGGGTG
  AACAGCATAAAACCTGTATGCTCAATGCCACATCAAAATTCATGGAGTTCTACCAAGATGTAAAGGACTG
  GTGGCTCTTCGGGTTCTATTTCTGTATGCCCTTGGTGTGCACTGCGATCTTCTACACCCTCATGACTTGT
  GAGATGTTGAACAGAAGGAATGGCAGCTTGAGAATTGCCCTCAGTGAACATCTTAAGCAGCGTCGAGAAG
  TGGCAAAAACAGTTTTCTGCTTGGTTGTAATTTTTGCTCTTTGCTGGTTCCCTGTTCATTTAAGCCGTAT
  ATTGAAGAAAACTGTGTATAACGAGATGGACAAGAACCGATGTGAATTACTTAGTTTCTTACTGCTCATG
  GATTACATCGGTATTAACTTGGCAACCATGAATTCATGTATAAACCCCATAGCTCTGTATTTTGTGAGCA
  AGAAATTTAAAAATTGTTTCCAGTCATGCCTCTGCTGCTGCTGTTACCAGTCCAAAAGTCTGATGACCTC
  GGTCCCCATGAACGGAACAAGCATCCAGTGGAAGGACCACGATCAAAACAACCACAACACAGACCGGAGC
  AGCCATAAGGACAGCATGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205385 representing NM_001957
  Red=Cloning site Green=Tags(s)
  
  METLCLRASFWLALVGCVISDNPERYSTNLSNHVDDFTTFRGTELSFLVTTHQPTNLVLPSNGSMHNYCP
  QQTKITSAFKYINTVISCTIFIVGMVGNATLLRIIYQNKCMRNGHNALIASLALGDLIYVVIDLPINVFK
  LLAGRWPFDHNDFGVFLCKLFPFLQKSSVGITVLNLCALSVDRYRAVASWSRVQGIGIPLVTAIEIVSIW
  ILSFILAIPEAIGFVMVPFEYRGEQHKTCMLNATSKFMEFYQDVKDWWLFGFYFCMPLVCTAIFYTLMTC
  EMLNRRNGSLRIALSEHLKQRREVAKTVFCLVVIFALCWFPVHLSRILKKTVYNEMDKNRCELLSFLLLM
  DYIGINLATMNSCINPIALYFVSKKFKNCFQSCLCCCCYQSKSLMTSVPMNGTSIQWKDHDQNNHNTDRS
  SHKDSMN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001957
• ORF Size: 1281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001957.1, NP_001948.1
• RefSeq Size: 4105 bp
• RefSeq ORF: 1284 bp
• Locus ID: 1909
• UniProt ID: P25101
• Cytogenetics: 4q31.22-q31.23
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
• Gene Summary: This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]