RNase H2 subunit C (RNASEH2C) (NM_032193) Human Tagged ORF Clone

CAT#: RG204827

RNASEH2C (tGFP-tagged) - Human ribonuclease H2, subunit C (RNASEH2C)

Product Images

Circular map for RG204827

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RNase H2 subunit C
• Synonyms: AGS3; AYP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204827 representing NM_032193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCGGCGACGAAGCGGCCATCGAGAGGCACCGCGTCCACTTGCGCTCCGCCACATTGCGCGACG
  CCGTACCCGCCACACTGCATCTGCTGCCCTGCGAGGTTGCGGTGGACGGGCCCGCCCCGGTGGGGCGCTT
  CTTCACGCCCGCCATCCGCCAGGGCCCCGAGGGACTCGAAGTGTCGTTTCGGGGCCGCTGTCTACGGGGA
  GAGGAGGTGGCGGTGCCGCCTGGCCTCGTGGGATACGTGATGGTGACAGAAGAGAAGAAGGTGTCGATGG
  GGAAGCCAGACCCCTTGCGGGATTCCGGGACTGACGACCAAGAGGAGGAGCCGCTGGAGCGGGACTTCGA
  CCGCTTCATTGGAGCCACTGCCAACTTCAGCCGCTTCACCCTGTGGGGTCTGGAGACCATCCCTGGCCCG
  GATGCCAAAGTGCGTGGGGCCTTAACTTGGCCCAGCCTTGCGGCAGCGATTCACGCACAGGTGCCCGAGG
  AC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204827 representing NM_032193
  Red=Cloning site Green=Tags(s)
  
  MESGDEAAIERHRVHLRSATLRDAVPATLHLLPCEVAVDGPAPVGRFFTPAIRQGPEGLEVSFRGRCLRG
  EEVAVPPGLVGYVMVTEEKKVSMGKPDPLRDSGTDDQEEEPLERDFDRFIGATANFSRFTLWGLETIPGP
  DAKVRGALTWPSLAAAIHAQVPED
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032193
• ORF Size: 492 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032193.4
• RefSeq Size: 2785 bp
• RefSeq ORF: 495 bp
• Locus ID: 84153
• UniProt ID: Q8TDP1
• Cytogenetics: 11q13.1
• Protein Pathways: DNA replication
• Gene Summary: This gene encodes a ribonuclease H subunit that can cleave ribonucleotides from RNA:DNA duplexes. Mutations in this gene cause Aicardi-Goutieres syndrome-3, a disease that causes severe neurologic dysfunction. A pseudogene for this gene has been identified on chromosome Y, near the sex determining region Y (SRY) gene. [provided by RefSeq, Jul 2008]