MS4A4A (NM_148975) Human Tagged ORF Clone

CAT#: RG204646

MS4A4A (tGFP-tagged) - Human membrane-spanning 4-domains, subfamily A, member 4 (MS4A4A), transcript variant 2

Product Images

Circular map for RG204646

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MS4A4A
• Synonyms: 4SPAN1; CD20-L1; CD20L1; HDCME31P; MS4A4; MS4A7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204646 representing NM_148975
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCAGACCTACAGCAGACATTGCAGGCCTGAAGAAAGCACCTTTTCTGCTGCCATGACAACCATGC
  AAGGAATGGAACAGGCCATGCCAGGGGCTGGCCCTGGTGTGCCCCAGCTGGGAAACATGGCTGTCATACA
  TTCACATCTGTGGAAAGGATTGCAAGAGAAGTTCTTGAAGGGAGAACCCAAAGTCCTTGGGGTTGTGCAG
  ATTCTGACTGCCCTGATGAGCCTTAGCATGGGAATAACAATGATGTGTATGGCATCTAATACTTATGGAA
  GTAACCCTATTTCCGTGTATATCGGGTACACAATTTGGGGGTCAGTAATGTTTATTATTTCAGGATCCTT
  GTCAATTGCAGCAGGAATTAGAACTACAAAAGGCCTGGTCCGAGGTAGTCTAGGAATGAATATCACCAGC
  TCTGTACTGGCTGCATCAGGGATCTTAATCAACACATTTAGCTTGGCGTTTTATTCATTCCATCACCCTT
  ACTGTAACTACTATGGCAACTCAAATAATTGTCATGGGACTATGTCCATCTTAATGGGTCTGGATGGCAT
  GGTGCTCCTCTTAAGTGTGCTGGAATTCTGCATTGCTGTGTCCCTCTCTGCCTTTGGATGTAAAGTGCTC
  TGTTGTACCCCTGGTGGGGTTGTGTTAATTCTGCCATCACATTCTCACATGGCAGAAACAGCATCTCCCA
  CACCACTTAATGAGGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204646 representing NM_148975
  Red=Cloning site Green=Tags(s)
  
  MHQTYSRHCRPEESTFSAAMTTMQGMEQAMPGAGPGVPQLGNMAVIHSHLWKGLQEKFLKGEPKVLGVVQ
  ILTALMSLSMGITMMCMASNTYGSNPISVYIGYTIWGSVMFIISGSLSIAAGIRTTKGLVRGSLGMNITS
  SVLAASGILINTFSLAFYSFHHPYCNYYGNSNNCHGTMSILMGLDGMVLLLSVLEFCIAVSLSAFGCKVL
  CCTPGGVVLILPSHSHMAETASPTPLNEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148975
• ORF Size: 717 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148975.2
• RefSeq Size: 1543 bp
• RefSeq ORF: 720 bp
• Locus ID: 51338
• UniProt ID: Q96JQ5
• Cytogenetics: 11q12.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features, similar intron/exon splice boundaries, and display unique expression patterns in hematopoietic cells and nonlymphoid tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]