HSD3B1 (NM_000862) Human Tagged ORF Clone

CAT#: RG204497

HSD3B1 (tGFP-tagged) - Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1)

Product Images

Circular map for RG204497

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HSD3B1
• Synonyms: 3BETAHSD; HSD3B; HSDB3; HSDB3A; SDR11E1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204497 representing NM_000862
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGGCTGGAGCTGCCTTGTGACAGGAGCAGGAGGGTTTCTGGGACAGAGGATCATCCGCCTCTTGG
  TGAAGGAGAAGGAGCTGAAGGAGATCAGGGTCTTGGACAAGGCCTTCGGACCAGAATTGAGAGAGGAATT
  TTCTAAACTCCAGAACAAGACCAAGCTGACAGTGCTGGAAGGAGACATTCTGGATGAGCCATTCCTGAAG
  AGAGCCTGCCAGGACGTCTCGGTCATCATCCACACCGCCTGTATCATTGATGTCTTCGGTGTCACTCACA
  GAGAGTCTATCATGAATGTCAATGTGAAAGGTACCCAGCTCCTGTTAGAGGCCTGTGTCCAAGCTAGTGT
  GCCAGTCTTCATCTACACCAGTAGCATAGAGGTAGCCGGGCCCAACTCCTACAAGGAAATCATCCAGAAT
  GGCCATGAAGAAGAGCCTCTGGAAAACACATGGCCCGCTCCATACCCACACAGCAAAAAGCTTGCTGAGA
  AGGCTGTACTGGCGGCTAACGGGTGGAATCTGAAAAACGGCGGCACCCTGTACACTTGTGCCTTACGACC
  CATGTATATCTATGGGGAAGGAAGCCGATTCCTTTCTGCTAGTATAAACGAGGCCCTGAACAACAATGGG
  ATCCTGTCAAGTGTTGGAAAGTTCTCCACTGTTAACCCAGTCTATGTTGGCAATGTGGCCTGGGCCCACA
  TTCTGGCCTTGAGGGCCCTGCAGGACCCCAAGAAGGCCCCAAGCATCCGAGGACAGTTCTACTATATCTC
  AGATGACACGCCTCACCAAAGCTATGATAACCTTAATTACACCCTGAGCAAAGAGTTCGGCCTCCGCCTT
  GATTCCAGATGGAGCTTTCCTTTATCCCTGATGTATTGGATTGGCTTCCTGCTGGAAATAGTGAGCTTCC
  TACTCAGGCCAATTTACACCTATCGACCGCCCTTCAACCGCCACATAGTCACATTGTCAAATAGCGTATT
  CACCTTCTCTTATAAGAAGGCTCAGCGAGATTTGGCGTATAAGCCACTCTACAGCTGGGAGGAAGCCAAG
  CAGAAAACGGTGGAGTGGGTTGGTTCCCTTGTGGACCGGCACAAGGAGAACCTGAAGTCCAAGACTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204497 representing NM_000862
  Red=Cloning site Green=Tags(s)
  
  MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLK
  RACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQN
  GHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNG
  ILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRL
  DSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAK
  QKTVEWVGSLVDRHKENLKSKTQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000862
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000862.2, NP_000853.1
• RefSeq Size: 1689 bp
• RefSeq ORF: 1122 bp
• Locus ID: 3283
• UniProt ID: P14060
• Cytogenetics: 1p12
• Domains: 3Beta_HSD
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016]