Glucose Transporter GLUT3 (SLC2A3) (NM_006931) Human Tagged ORF Clone

CAT#: RG204430

SLC2A3 (tGFP-tagged) - Human solute carrier family 2 (facilitated glucose transporter), member 3 (SLC2A3)

Product Images

Circular map for RG204430

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Glucose Transporter GLUT3
• Synonyms: GLUT3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204430 representing NM_006931
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACACAGAAGGTCACCCCAGCTCTGATATTTGCCATCACAGTTGCTACAATCGGCTCTTTCCAAT
  TTGGCTACAACACTGGGGTCATCAATGCTCCTGAGAAGATCATAAAGGAATTTATCAATAAAACTTTGAC
  GGACAAGGGAAATGCCCCACCCTCTGAGGTGCTGCTCACGTCTCTCTGGTCCTTGTCTGTGGCCATATTT
  TCCGTCGGGGGTATGATCGGCTCCTTTTCCGTCGGACTCTTCGTCAACCGCTTTGGCAGGCGCAATTCAA
  TGCTGATTGTCAACCTGTTGGCTGTCACTGGTGGCTGCTTTATGGGACTGTGTAAAGTAGCTAAGTCGGT
  TGAAATGCTGATCCTGGGTCGCTTGGTTATTGGCCTCTTCTGCGGACTCTGCACAGGTTTTGTGCCCATG
  TACATTGGAGAGATCTCGCCTACTGCCCTGCGGGGTGCCTTTGGCACTCTCAACCAGCTGGGCATCGTTG
  TTGGAATTCTGGTGGCCCAGATCTTTGGTCTGGAATTCATCCTTGGGTCTGAAGAGCTATGGCCGCTGCT
  ACTGGGTTTTACCATCCTTCCTGCTATCCTACAAAGTGCAGCCCTTCCATTTTGCCCTGAAAGTCCCAGA
  TTTTTGCTCATTAACAGAAAAGAAGAGGAGAATGCTAAGCAGATCCTCCAGCGGTTGTGGGGCACCCAGG
  ATGTATCCCAAGACATCCAGGAGATGAAAGATGAGAGTGCAAGGATGTCACAAGAAAAGCAAGTCACCGT
  GCTAGAGCTCTTTAGAGTGTCCAGCTACCGACAGCCCATCATCATTTCCATTGTGCTCCAGCTCTCTCAG
  CAGCTCTCTGGGATCAATGCTGTGTTCTATTACTCAACAGGAATCTTCAAGGATGCAGGTGTTCAAGAGC
  CCATCTATGCCACCATCGGCGCGGGTGTGGTTAATACTATCTTCACTGTAGTTTCTCTATTTCTGGTGGA
  AAGGGCAGGAAGAAGGACTCTGCATATGATAGGCCTTGGAGGGATGGCTTTTTGTTCCACGCTCATGACT
  GTTTCTTTGTTATTAAAGGATAACTATAATGGGATGAGCTTTGTCTGTATTGGGGCTATCTTGGTCTTTG
  TAGCCTTCTTTGAAATTGGACCAGGCCCCATTCCCTGGTTTATTGTGGCCGAACTCTTCAGCCAGGGCCC
  CCGCCCAGCTGCGATGGCAGTGGCCGGCTGCTCCAACTGGACCTCCAACTTCCTAGTCGGATTGCTCTTC
  CCCTCCGCTGCTCACTATTTAGGAGCCTACGTTTTTATTATCTTCACCGGCTTCCTCATTACCTTCTTGG
  CTTTTACCTTCTTCAAAGTCCCTGAGACCCGTGGCAGGACTTTTGAGGATATCACACGGGCCTTTGAAGG
  GCAGGCACACGGTGCAGATAGATCTGGAAAGGACGGCGTCATGGAGATGAACAGCATCGAGCCTGCTAAG
  GAGACCACCACCAATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204430 representing NM_006931
  Red=Cloning site Green=Tags(s)
  
  MGTQKVTPALIFAITVATIGSFQFGYNTGVINAPEKIIKEFINKTLTDKGNAPPSEVLLTSLWSLSVAIF
  SVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPM
  YIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSAALPFCPESPR
  FLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQ
  QLSGINAVFYYSTGIFKDAGVQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMT
  VSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLF
  PSAAHYLGAYVFIIFTGFLITFLAFTFFKVPETRGRTFEDITRAFEGQAHGADRSGKDGVMEMNSIEPAK
  ETTTNV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006931
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006931.3
• RefSeq Size: 3915 bp
• RefSeq ORF: 1491 bp
• Locus ID: 6515
• UniProt ID: P11169
• Cytogenetics: 12p13.31
• Domains: sugar_tr
• Protein Families: Transmembrane
• Gene Summary: Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:9477959, PubMed:26176916). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:9477959, PubMed:26176916). Does not mediate fructose transport (PubMed:9477959, PubMed:26176916).[UniProtKB/Swiss-Prot Function]