DYNC2I2 (NM_052844) Human Tagged ORF Clone

CAT#: RG204288

WDR34 (tGFP-tagged) - Human WD repeat domain 34 (WDR34)

Product Images

Circular map for RG204288

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DYNC2I2
• Synonyms: bA216B9.3; CFAP133; DIC5; FAP133; SRTD11; WDR34
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204288 representing NM_052844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCCGCGCGCAGCCGGGGCCACTCAGCCAGGCGGGAAGCGCTGGTGTTGCGGCGCTGGCGACAG
  TCGGGGTTGCGAGCGGCCCGGGGCCGGGGCGGCCAGGGCCGCTGCAGGACGAGACCCTGGGTGTGGCGTC
  CGTGCCCTCGCAGTGGAGGGCCGTCCAGGGCATCCGCGGGGAGACGAAAAGTTGCCAGACGGCCAGCATT
  GCCACTGCCAGTGCATCCGCCCAGGCCAGGAATCATGTGGACGCCCAGGTGCAGACGGAGGCCCCCGTGC
  CTGTCAGCGTGCAGCCCCCGTCCCAGTACGACATACCCAGGCTTGCAGCCTTTCTTCGGAGAGTGGAGGC
  CATGGTCATCCGAGAGCTGAACAAGAATTGGCAGAGCCACGCGTTTGATGGCTTCGAGGTGAACTGGACC
  GAGCAGCAGCAGATGGTGTCTTGTCTGTATACCCTGGGCTACCCGCCAGCCCAAGCGCAGGGTCTGCATG
  TGACCAGCATCTCCTGGAACTCCACTGGCTCTGTGGTGGCCTGTGCCTACGGCCGGCTGGACCATGGGGA
  CTGGAGCACGCTTAAGTCCTTCGTGTGTGCCTGGAACCTGGACCGGCGAGACCTGCGTCCCCAGCAACCG
  TCGGCCGTGGTGGAGGTCCCCAGCGCTGTCCTGTGTCTGGCCTTCCACCCCACGCAGCCCTCCCACGTCG
  CAGGAGGGCTGTACAGTGGTGAGGTGTTGGTGTGGGACCTGAGCCGTCTTGAGGACCCGCTGCTGTGGCG
  CACAGGCCTGACGGATGACACCCACACAGACCCTGTGTCCCAGGTGGTGTGGCTGCCCGAGCCTGGGCAC
  AGCCACCGCTTCCAGGTGCTGAGTGTGGCCACTGACGGGAAGGTGCTACTCTGGCAGGGCATCGGGGTAG
  GCCAGCTGCAGCTCACAGAGGGCTTCGCCCTGGTCATGCAGCAGCTGCCACGGAGCACCAAGCTCAAGAA
  GCATCCCCGCGGGGAGACCGAGGTGGGCGCCACGGCAGTGGCCTTCTCCAGCTTTGACCCTAGGCTGTTC
  ATTCTGGGCACGGAAGGCGGCTTCCCGCTCAAGTGTTCCCTGGCAGCTGGAGAGGCAGCCCTCACGCGGA
  TGCCCAGCTCCGTGCCCCTGCGGGCCCCAGCACAGTTTACCTTCTCCCCCCACGGCGGTCCCATCTACTC
  TGTGAGCTGTTCCCCCTTCCACAGGAATCTCTTCCTGAGCGCTGGGACTGACGGGCATGTCCACCTGTAC
  TCCATGCTGCAGGCCCCTCCCTTGACTTCGCTGCAGCTCTCCCTCAAGTATCTGTTTGCTGTGCGCTGGT
  CCCCAGTGCGGCCCTTGGTTTTTGCAGCTGCCTCTGGGAAAGGTGACGTGCAGCTGTTTGATCTCCAGAA
  AAGCTCCCAGAAACCCACAGTTTTGATCAAGCAAACCCAGGATGAAAGCCCTGTCTACTGTCTGGAGTTC
  AACAGCCAGCAGACTCAGCTCTTGGCTGCGGGCGATGCCCAGGGCACAGTGAAGGTGTGGCAGCTGAGCA
  CAGAGTTCACGGAACAAGGGCCCCGGGAAGCTGAGGACCTGGACTGCCTGGCAGCAGAGGTGGCGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204288 representing NM_052844
  Red=Cloning site Green=Tags(s)
  
  MATRAQPGPLSQAGSAGVAALATVGVASGPGPGRPGPLQDETLGVASVPSQWRAVQGIRGETKSCQTASI
  ATASASAQARNHVDAQVQTEAPVPVSVQPPSQYDIPRLAAFLRRVEAMVIRELNKNWQSHAFDGFEVNWT
  EQQQMVSCLYTLGYPPAQAQGLHVTSISWNSTGSVVACAYGRLDHGDWSTLKSFVCAWNLDRRDLRPQQP
  SAVVEVPSAVLCLAFHPTQPSHVAGGLYSGEVLVWDLSRLEDPLLWRTGLTDDTHTDPVSQVVWLPEPGH
  SHRFQVLSVATDGKVLLWQGIGVGQLQLTEGFALVMQQLPRSTKLKKHPRGETEVGATAVAFSSFDPRLF
  ILGTEGGFPLKCSLAAGEAALTRMPSSVPLRAPAQFTFSPHGGPIYSVSCSPFHRNLFLSAGTDGHVHLY
  SMLQAPPLTSLQLSLKYLFAVRWSPVRPLVFAAASGKGDVQLFDLQKSSQKPTVLIKQTQDESPVYCLEF
  NSQQTQLLAAGDAQGTVKVWQLSTEFTEQGPREAEDLDCLAAEVAA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052844
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052844.3, NP_443076.2
• RefSeq Size: 1818 bp
• RefSeq ORF: 1611 bp
• Locus ID: 89891
• UniProt ID: Q96EX3
• Cytogenetics: 9q34.11
• Domains: WD40
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Defects in this gene are a cause of short-rib thoracic dysplasia 11 with or without polydactyly. [provided by RefSeq, Mar 2014]