MFSD2A (NM_032793) Human Tagged ORF Clone

CAT#: RG204264

MFSD2A (tGFP-tagged) - Human major facilitator superfamily domain containing 2A (MFSD2A), transcript variant 2

Product Images

Circular map for RG204264

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MFSD2A
• Synonyms: MCPH15; MFSD2; NEDMISBA; NLS1; SLC59A1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204264 representing NM_032793
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAAGGAGAAGGCGCCGAGAGCGGCTCCGCGGCGGGGCTGCTACCCACCAGCATCCTCCAAAGCA
  CTGAACGCCCGGCCCAGGTGAAGAAAGAACCGAAAAAGAAGAAACAACAGTTGTCTGTTTGCAACAAGCT
  TTGCTATGCACTTGGGGGAGCCCCCTACCAGGTGACGGGCTGTGCCCTGGGTTTCTTCCTTCAGATCTAC
  CTATTGGATGTGGCTCAGGTGGGCCCTTTCTCTGCCTCCATCATCCTGTTTGTGGGCCGAGCCTGGGATG
  CCATCACAGACCCCCTGGTGGGCCTCTGCATCAGCAAATCCCCCTGGACCTGCCTGGGTCGCCTTATGCC
  CTGGATCATCTTCTCCACGCCCCTGGCCGTCATTGCCTACTTCCTCATCTGGTTCGTGCCCGACTTCCCA
  CACGGCCAGACCTATTGGTACCTGCTTTTCTATTGCCTCTTTGAAACAATGGTCACGTGTTTCCATGTTC
  CCTACTCGGCTCTCACCATGTTCATCAGCACCGAGCAGACTGAGCGGGATTCTGCCACCGCCTATCGGAT
  GACTGTGGAAGTGCTGGGCACAGTGCTGGGCACGGCGATCCAGGGACAAATCGTGGGCCAAGCAGACACG
  CCTTGTTTCCAGGACCTCAATAGCTCTACAGTAGCTTCACAAAGTGCCAACCATACACATGGCACCACCT
  CACACAGGGAAACGCAAAAGGCATACCTGCTGGCAGCGGGGGTCATTGTCTGTATCTATATAATCTGTGC
  TGTCATCCTGATCCTGGGCGTGCGGGAGCAGAGAGAACCCTATGAAGCCCAGCAGTCTGAGCCAATCGCC
  TACTTCCGGGGCCTACGGCTGGTCATGAGCCACGGCCCATACATCAAACTTATTACTGGCTTCCTCTTCA
  CCTCCTTGGCTTTCATGCTGGTGGAGGGGAACTTTGTCTTGTTTTGCACCTACACCTTGGGCTTCCGCAA
  TGAATTCCAGAATCTACTCCTGGCCATCATGCTCTCGGCCACTTTAACCATTCCCATCTGGCAGTGGTTC
  TTGACCCGGTTTGGCAAGAAGACAGCTGTATATGTTGGGATCTCATCAGCAGTGCCATTTCTCATCTTGG
  TGGCCCTCATGGAGAGTAACCTCATCATTACATATGCGGTAGCTGTGGCAGCTGGCATCAGTGTGGCAGC
  TGCCTTCTTACTACCCTGGTCCATGCTGCCTGATGTCATTGACGACTTCCATCTGAAGCAGCCCCACTTC
  CATGGAACCGAGCCCATCTTCTTCTCCTTCTATGTCTTCTTCACCAAGTTTGCCTCTGGAGTGTCACTGG
  GCATTTCTACCCTCAGTCTGGACTTTGCAGGGTACCAGACCCGTGGCTGCTCGCAGCCGGAACGTGTCAA
  GTTTACACTGAACATGCTCGTGACCATGGCTCCCATAGTTCTCATCCTGCTGGGCCTGCTGCTCTTCAAA
  ATGTACCCCATTGATGAGGAGAGGCGGCGGCAGAATAAGAAGGCCCTGCAGGCACTGAGGGACGAGGCCA
  GCAGCTCTGGCTGCTCAGAAACAGACTCCACAGAGCTGGCTAGCATCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204264 representing NM_032793
  Red=Cloning site Green=Tags(s)
  
  MAKGEGAESGSAAGLLPTSILQSTERPAQVKKEPKKKKQQLSVCNKLCYALGGAPYQVTGCALGFFLQIY
  LLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPLAVIAYFLIWFVPDFP
  HGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIVGQADT
  PCFQDLNSSTVASQSANHTHGTTSHRETQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIA
  YFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWF
  LTRFGKKTAVYVGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHF
  HGTEPIFFSFYVFFTKFASGVSLGISTLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFK
  MYPIDEERRRQNKKALQALRDEASSSGCSETDSTELASIL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032793
• ORF Size: 1590 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032793.5
• RefSeq Size: 2170 bp
• RefSeq ORF: 1593 bp
• Locus ID: 84879
• UniProt ID: Q8NA29
• Cytogenetics: 1p34.2
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene is a transmembrane protein and sodium-dependent lysophosphatidylcholine transporter. The encoded protein is involved in the establishment of the blood-brain barrier and is required for brain growth and function. Defects in this gene are a cause of a progressive microcephaly syndrome. [provided by RefSeq, Mar 2017]