LCAT (NM_000229) Human Tagged ORF Clone

CAT#: RG203763

LCAT (tGFP-tagged) - Human lecithin-cholesterol acyltransferase (LCAT)

Product Images

Circular map for RG203763

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LCAT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203763 representing NM_000229
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCGCCCGGCTCCCCATGGCAGTGGGTGACGCTGCTGCTGGGGCTGCTGCTCCCTCCTGCCGCCC
  CCTTCTGGCTCCTCAATGTGCTCTTCCCCCCGCACACCACGCCCAAGGCTGAGCTCAGTAACCACACACG
  GCCCGTCATCCTCGTGCCCGGCTGCCTGGGGAATCAGCTAGAAGCCAAGCTGGACAAACCAGATGTGGTG
  AACTGGATGTGCTACCGCAAGACAGAGGACTTCTTCACCATCTGGCTGGATCTCAACATGTTCCTACCCC
  TTGGGGTAGACTGCTGGATCGATAACACCAGGGTTGTCTACAACCGGAGCTCTGGGCTCGTGTCCAACGC
  CCCTGGTGTCCAGATCCGCGTCCCTGGCTTTGGCAAGACCTACTCTGTGGAGTACCTGGACAGCAGCAAG
  CTGGCAGGGTACCTGCACACACTGGTGCAGAACCTGGTCAACAATGGCTACGTGCGGGACGAGACTGTGC
  GCGCCGCCCCCTATGACTGGCGGCTGGAGCCCGGCCAGCAGGAGGAGTACTACCGCAAGCTCGCAGGGCT
  GGTGGAGGAGATGCACGCTGCCTATGGGAAGCCTGTCTTCCTCATTGGCCACAGCCTCGGCTGTCTACAC
  TTGCTCTATTTCCTGCTGCGCCAGCCCCAGGCCTGGAAGGACCGCTTTATTGATGGCTTCATCTCTCTTG
  GGGCTCCCTGGGGTGGCTCCATCAAGCCCATGCTGGTCTTGGCCTCAGGTGACAACCAGGGCATCCCCAT
  CATGTCCAGCATCAAGCTGAAAGAGGAGCAGCGCATAACCACCACCTCCCCCTGGATGTTTCCCTCTCGC
  ATGGCGTGGCCTGAGGACCACGTGTTCATTTCCACACCCAGCTTCAACTACACAGGCCGTGACTTCCAAC
  GCTTCTTTGCAGACCTGCACTTTGAGGAAGGCTGGTACATGTGGCTGCAGTCACGTGACCTCCTGGCAGG
  ACTCCCAGCACCTGGTGTGGAAGTATACTGTCTTTACGGCGTGGGCCTGCCCACGCCCCGCACCTACATC
  TACGACCACGGCTTCCCCTACACGGACCCTGTGGGTGTGCTCTATGAGGATGGTGATGACACGGTGGCGA
  CCCGCAGCACCGAGCTCTGTGGCCTGTGGCAGGGCCGCCAGCCACAGCCTGTGCACCTGCTGCCCCTGCA
  CGGGATACAGCATCTCAACATGGTCTTCAGCAACCTGACCCTGGAGCACATCAATGCCATCCTGCTGGGT
  GCCTACCGCCAGGGTCCCCCTGCATCCCCGACTGCCAGCCCAGAGCCCCCGCCTCCTGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203763 representing NM_000229
  Red=Cloning site Green=Tags(s)
  
  MGPPGSPWQWVTLLLGLLLPPAAPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVV
  NWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSK
  LAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLH
  LLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSR
  MAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYI
  YDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLG
  AYRQGPPASPTASPEPPPPE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000229
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000229.2
• RefSeq Size: 1354 bp
• RefSeq ORF: 1323 bp
• Locus ID: 3931
• UniProt ID: P04180
• Cytogenetics: 16q22.1
• Domains: LACT
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]