R3HDM4 (NM_138774) Human Tagged ORF Clone

CAT#: RG203619

R3HDM4 (tGFP-tagged) - Human chromosome 19 open reading frame 22 (C19orf22)

Product Images

Circular map for RG203619

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: R3HDM4
• Synonyms: C19orf22
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203619 representing NM_138774
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGCGCTGGAGAACCCCGAGTGCGGCCCGGAGGCGGCGGAGGGCACCCCGGGCGGGCGGCGGCTGC
  TGCCCCTTCCCAGCTGCCTGCCTGCCCTAGCCAGCTCCCAGGTGAAGAGACTCTCGGCTTCCAGGCGGAA
  ACAGCACTTCATCAACCAGGCAGTGCGGAACTCAGACCTCGTGCCCAAGGCCAAGGGGCGGAAGAGCCTC
  CAGCGCCTGGAGAACACCCAGTACCTCCTGACCCTGCTGGAGACAGACGGGGGCCTGCCTGGCCTGGAGG
  ATGGGGACTTGGCACCCCCTGCATCACCAGGCATCTTTGCCGAGGCCTGCAACAACGCCACCTATGTGGA
  GGTCTGGAACGATTTCATGAACCGCTCCGGGGAGGAGCAGGAGCGGGTTCTTCGCTACCTGGAGGATGAG
  GGCAGGAGCAAGGCGCGGAGGAGGGGCCCTGGCCGTGGGGAGGACCGGAGGAGAGAGGACCCCGCCTATA
  CACCCCGCGAGTGCTTCCAGCGCATCAGCCGGCGTCTGCGAGCCGTCCTCAAGCGCAGCCGCATCCCCAT
  GGAAACGCTGGAGACCTGGGAGGAGCGGCTGCTTCGGTTCTTCTCCGTGTCCCCCCAGGCCGTGTACACA
  GCAATGCTAGACAACAGCTTCGAGAGGCTTCTGCTGCACGCTGTCTGCCAGTACATGGACCTCATCTCGG
  CCAGTGCTGACCTGGAGGGGAAGCGGCAGATGAAGGTCAGTAATCGGCACCTGGATTTCCTGCCGCCGGG
  GCTGCTCCTGTCCGCCTACCTGGAGCAGCACAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203619 representing NM_138774
  Red=Cloning site Green=Tags(s)
  
  MVALENPECGPEAAEGTPGGRRLLPLPSCLPALASSQVKRLSASRRKQHFINQAVRNSDLVPKAKGRKSL
  QRLENTQYLLTLLETDGGLPGLEDGDLAPPASPGIFAEACNNATYVEVWNDFMNRSGEEQERVLRYLEDE
  GRSKARRRGPGRGEDRRREDPAYTPRECFQRISRRLRAVLKRSRIPMETLETWEERLLRFFSVSPQAVYT
  AMLDNSFERLLLHAVCQYMDLISASADLEGKRQMKVSNRHLDFLPPGLLLSAYLEQHS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138774
• ORF Size: 804 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138774.4
• RefSeq Size: 1810 bp
• RefSeq ORF: 807 bp
• Locus ID: 91300
• UniProt ID: Q96D70
• Cytogenetics: 19p13.3