SEPSECS (NM_016955) Human Tagged ORF Clone

CAT#: RG203211

SEPSECS (tGFP-tagged) - Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS)

Product Images

Circular map for RG203211

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SEPSECS
• Synonyms: LP; PCH2D; SLA; SLA/LP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203211 representing NM_016955
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACCTCATACGGCTGCTTCTGGAGAAGGTTCATTCATGGCATTGGACGATCCGGTGATATTTCTG
  CTGTGCAACCAAAAGCTGCAGGCTCTAGCCTTTTGAACAAAATTACCAATTCTTTGGTCCTGGACATTAT
  AAAGCTGGCTGGTGTCCATACAGTAGCCAACTGCTTTGTAGTTCCTATGGCAACTGGTATGAGTCTAACT
  CTGTGTTTCTTAACATTACGACACAAAAGACCAAAGGCAAAGTATATTATATGGCCACGAATAGACCAGA
  AGTCCTGCTTTAAATCCATGATCACTGCAGGTTTTGAGCCTGTGGTGATAGAAAATGTTTTGGAAGGTGA
  CGAGCTGCGTACAGACCTGAAAGCAGTGGAGGCTAAAGTCCAGGAACTTGGGCCTGATTGCATTCTGTGT
  ATTCATTCTACTACATCCTGTTTTGCTCCAAGGGTGCCTGATAGATTAGAAGAACTGGCTGTGATTTGTG
  CTAATTATGACATTCCACATATAGTTAATAATGCTTATGGAGTGCAGTCTTCAAAGTGTATGCATCTCAT
  TCAGCAGGGGGCTCGAGTTGGTAGAATAGATGCTTTTGTTCAGAGCTTGGACAAAAATTTTATGGTTCCA
  GTAGGTGGTGCTATAATTGCTGGCTTTAATGATTCATTCATTCAGGAAATCAGCAAGATGTATCCAGGAA
  GAGCTTCAGCTTCACCTTCTTTAGATGTCCTTATTACTTTATTGTCACTTGGATCAAATGGCTATAAGAA
  GCTACTAAAAGAAAGAAAGGAAATGTTTTCATATTTGTCCAACCAAATAAAGAAGTTGTCAGAAGCCTAC
  AATGAAAGACTGTTGCATACACCTCACAATCCCATATCTTTAGCTATGACACTTAAAACACTAGATGAAC
  ACCGTGACAAAGCTGTCACTCAGCTTGGCTCGATGCTTTTTACCAGACAGGTTTCTGGAGCCAGGGTTGT
  GCCTCTTGGGTCCATGCAAACTGTGAGTGGCTATACTTTCAGAGGCTTTATGTCACATACAAATAATTAC
  CCTTGTGCTTACCTCAATGCTGCATCAGCCATCGGAATGAAGATGCAGGATGTGGACCTGTTCATAAAGA
  GACTTGACAGGTGTTTAAAGGCAGTAAGAAAAGAACGAAGTAAAGAGAGTGATGACAATTATGACAAAAC
  TGAAGATGTGGATATTGAAGAAATGGCTTTAAAACTAGATAATGTACTTCTTGACACATACCAGGATGCT
  TCTTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203211 representing NM_016955
  Red=Cloning site Green=Tags(s)
  
  MSTSYGCFWRRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAGVHTVANCFVVPMATGMSLT
  LCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCILC
  IHSTTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVP
  VGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLSNQIKKLSEAY
  NERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNY
  PCAYLNAASAIGMKMQDVDLFIKRLDRCLKAVRKERSKESDDNYDKTEDVDIEEMALKLDNVLLDTYQDA
  SS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016955
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016955.2, NP_058651.2
• RefSeq Size: 1269 bp
• RefSeq ORF: 1506 bp
• Locus ID: 51091
• UniProt ID: Q9HD40
• Cytogenetics: 4p15.2
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]