POLR3F (NM_006466) Human Tagged ORF Clone

CAT#: RG203100

POLR3F (tGFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F)

Product Images

Circular map for RG203100

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POLR3F
• Synonyms: C34; RPC6; RPC39
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203100 representing NM_006466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGTGAAGGTGAAGGTGCAGCCGCCTGACGCGGATCCGGTCGAAATAGAAAACAGGATTATAG
  AATTATGTCACCAGTTCCCTCATGGAATCACAGACCAAGTAATTCAGAATGAAATGCCTCATATAGAAGC
  CCAGCAGCGGGCAGTAGCCATCAATAGGTTGTTGTCTATGGGTCAGTTGGATCTCTTAAGGAGCAATACG
  GGCCTTTTATATAGAATAAAGGACTCTCAGAATGCTGGTAAAATGAAGGGATCCGATAACCAAGAAAAAC
  TAGTATATCAAATCATAGAGGATGCAGGAAATAAAGGAATATGGAGCAGAGATATCCGCTATAAAAGTAA
  TTTGCCATTAACAGAAATCAACAAAATTCTGAAGAATCTGGAAAGTAAAAAGCTTATCAAAGCTGTTAAG
  TCTGTAGCAGCCTCAAAAAAGAAGGTGTATATGCTCTATAACCTGCAGCCAGACCGGTCTGTGACTGGTG
  GAGCCTGGTACAGTGACCAGGATTTTGAATCTGAATTTGTAGAGGTGCTTAACCAACAGTGTTTTAAATT
  CCTACAGTCCAAGGCAGAAACAGCACGAGAAAGCAAACAGAACCCAATGATACAAAGAAATAGTTCATTT
  GCCTCATCACATGAAGTGTGGAAATATATCTGCGAATTGGGAATCAGTAAGGTAGAGTTATCCATGGAAG
  ACATTGAAACCATCCTGAATACACTCATTTATGATGGAAAAGTGGAGATGACGATTATTGCTGCAAAAGA
  AGGCACAGTTGGCAGTGTAGATGGACACATGAAACTGTACAGGGCAGTCAATCCAATCATCCCTCCCACG
  GGTTTGGTCCGGGCACCCTGTGGACTCTGCCCGGTTTTTGATGACTGCCACGAAGGTGGTGAGATTTCAC
  CATCTAACTGTATTTACATGACAGAGTGGCTCGAATTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203100 representing NM_006466
  Red=Cloning site Green=Tags(s)
  
  MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNT
  GLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVK
  SVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSF
  ASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPIIPPT
  GLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006466
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006466.2, NP_006457.2
• RefSeq Size: 2177 bp
• RefSeq ORF: 951 bp
• Locus ID: 10621
• UniProt ID: Q9H1D9
• Cytogenetics: 20p11.23
• Domains: RNA_pol_Rpc34
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]