CYP39A1 (NM_016593) Human Tagged ORF Clone

CAT#: RG203058

CYP39A1 (tGFP-tagged) - Human cytochrome P450, family 39, subfamily A, polypeptide 1 (CYP39A1)

Product Images

Circular map for RG203058

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CYP39A1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203058 representing NM_016593
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTAATTTCCCCAACAGTGATTATAATCCTGGGTTGCCTTGCTCTGTTCTTACTCCTTCAGCCGA
  AGAATTTGCGTAGACCCCCGTGCATCAAGGGCTGGATTCCTTGGATTGGAGTTGGATTTGAGTTTGGGAA
  AGCCCCTCTAGAATTTATAGAGAAAGCAAGAATCAAGTATGGACCAATATTTACAGTCTTTGCTATGGGA
  AACCGAATGACCTTTGTTACTGAAGAAGAAGGAATTAATGTGTTTCTAAAATCCAAAAAAGTAGATTTTG
  AACTAGCAGTGCAAAATATCGTTTATCGTACAGCATCAATTCCAAAGAATGTCTTTTTAGCACTGCATGA
  AAAACTCTATATTATGTTGAAAGGGAAAATGGGGACTGTCAATCTCCATCAGTTTACTGGGCAACTGACT
  GAAGAATTACATGAACAACTGGAGAATTTAGGCACTCATGGGACAATGGACCTGAACAACTTAGTAAGAC
  ATCTCCTTTATCCAGTCACAGTGAATATGCTCTTTAATAAAAGTTTGTTTTCCACAAACAAGAAAAAAAT
  CAAGGAGTTCCATCAGTATTTTCAAGTTTATGATGAAGATTTTGAGTATGGGTCCCAGTTGCCAGAGTGT
  CTTCTAAGAAACTGGTCAAAATCCAAAAAGTGGTTCCTGGAACTGTTTGAGAAAAACATTCCAGATATAA
  AAGCATGTAAATCTGCAAAAGATAATTCCATGACATTATTGCAAGCTACGCTGGATATTGTAGAGACGGA
  AACAAGTAAGGAAAACTCACCCAATTATGGGCTCTTACTGCTTTGGGCTTCTCTGTCTAATGCTGTTCCT
  GTTGCATTTTGGACACTTGCACACGTCCTTTCTCATCCTGATATCCACAAGGCCATTATGGAAGGCATAT
  CTTCTGTGTTTGGCAAAGCAGGCAAAGATAAGATTAAAGTGTCTGAGGATGACCTGGAGAATCTCCTTCT
  AATTAAATGGTGTGTTTTGGAAACCATTCGTTTAAAAGCTCCTGGTGTCATTACTAGAAAAGTGGTGAAG
  CCTGTGGAAATTTTGAATTACATCATTCCTTCTGGTGACTTGTTGATGTTGTCTCCATTTTGGCTGCATA
  GAAATCCAAAGTATTTTCCTGAGCCTGAATTGTTCAAACCTGAACGTTGGAAAAAGGCAAATTTAGAGAA
  GCACTCTTTCTTGGACTGCTTCATGGCATTTGGAAGCGGGAAGTTCCAGTGTCCTGCAAGGTGGTTTGCT
  CTGTTAGAGGTTCAGATGTGTATTATTTTAATACTTTATAAATATGACTGTAGTCTTCTGGACCCATTAC
  CCAAACAGAGTTATCTCCATTTGGTGGGTGTCCCCCAGCCGGAAGGGCAATGCCGAATTGAATATAAACA
  AAGAATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203058 representing NM_016593
  Red=Cloning site Green=Tags(s)
  
  MELISPTVIIILGCLALFLLLQPKNLRRPPCIKGWIPWIGVGFEFGKAPLEFIEKARIKYGPIFTVFAMG
  NRMTFVTEEEGINVFLKSKKVDFELAVQNIVYRTASIPKNVFLALHEKLYIMLKGKMGTVNLHQFTGQLT
  EELHEQLENLGTHGTMDLNNLVRHLLYPVTVNMLFNKSLFSTNKKKIKEFHQYFQVYDEDFEYGSQLPEC
  LLRNWSKSKKWFLELFEKNIPDIKACKSAKDNSMTLLQATLDIVETETSKENSPNYGLLLLWASLSNAVP
  VAFWTLAHVLSHPDIHKAIMEGISSVFGKAGKDKIKVSEDDLENLLLIKWCVLETIRLKAPGVITRKVVK
  PVEILNYIIPSGDLLMLSPFWLHRNPKYFPEPELFKPERWKKANLEKHSFLDCFMAFGSGKFQCPARWFA
  LLEVQMCIILILYKYDCSLLDPLPKQSYLHLVGVPQPEGQCRIEYKQRI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016593
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016593.3, NP_057677.2
• RefSeq Size: 2288 bp
• RefSeq ORF: 1410 bp
• Locus ID: 51302
• UniProt ID: Q9NYL5
• Cytogenetics: 6p12.3
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Primary bile acid biosynthesis
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is involved in the conversion of cholesterol to bile acids. Its substrates include the oxysterols 25-hydroxycholesterol, 27-hydroxycholesterol and 24-hydroxycholesterol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]