SULT1B1 (NM_014465) Human Tagged ORF Clone

CAT#: RG203053

SULT1B1 (tGFP-tagged) - Human sulfotransferase family, cytosolic, 1B, member 1 (SULT1B1)

Product Images

Circular map for RG203053

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SULT1B1
• Synonyms: ST1B1; ST1B2; SULT1B2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203053 representing NM_014465
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTCCCCAAAAGATATTCTGCGAAAAGATCTGAAGTTGGTCCATGGTTATCCCATGACCTGTGCTT
  TTGCAAGCAACTGGGAAAAAATTGAACAGTTCCATAGCAGACCAGATGACATTGTGATAGCCACTTATCC
  TAAATCAGGTACTACTTGGGTTAGTGAAATTATAGACATGATTCTAAATGATGGAGATATTGAAAAATGT
  AAGCGAGGTTTTATTACTGAAAAAGTTCCAATGTTGGAAATGACTCTCCCTGGATTAAGAACATCAGGTA
  TAGAACAATTGGAGAAGAATCCATCACCCCGGATTGTGAAAACACATCTACCGACTGATCTTCTTCCTAA
  ATCTTTCTGGGAAAACAATTGCAAGATGATTTATCTGGCTCGTAATGCCAAGGATGTTTCAGTCTCATAT
  TACCATTTTGACTTAATGAATAATTTACAGCCTTTTCCTGGTACCTGGGAAGAATATCTGGAGAAATTCT
  TAACTGGAAAAGTGGCCTATGGTTCCTGGTTTACTCATGTTAAAAACTGGTGGAAGAGAAAGGAAGAACA
  CCCAATACTTTTTTTGTACTATGAAGATATGAAAGAGAATCCAAAGGAGGAAATCAAGAAGATCATTAGA
  TTTCTAGAGAAGAACCTGAATGATGAGATCTTGGATAGGATCATCCATCACACCTCATTTGAAGTGATGA
  AGGACAATCCTTTGGTAAATTATACACATCTACCAACTACAGTGATGGATCATAGCAAATCCCCTTTTAT
  GCGTAAAGGGACGGCTGGTGACTGGAAGAATTACTTCACCGTGGCCCAAAATGAGAAATTTGATGCTATT
  TATGAGACAGAAATGTCCAAAACTGCACTTCAATTCCGCACAGAGATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203053 representing NM_014465
  Red=Cloning site Green=Tags(s)
  
  MLSPKDILRKDLKLVHGYPMTCAFASNWEKIEQFHSRPDDIVIATYPKSGTTWVSEIIDMILNDGDIEKC
  KRGFITEKVPMLEMTLPGLRTSGIEQLEKNPSPRIVKTHLPTDLLPKSFWENNCKMIYLARNAKDVSVSY
  YHFDLMNNLQPFPGTWEEYLEKFLTGKVAYGSWFTHVKNWWKRKEEHPILFLYYEDMKENPKEEIKKIIR
  FLEKNLNDEILDRIIHHTSFEVMKDNPLVNYTHLPTTVMDHSKSPFMRKGTAGDWKNYFTVAQNEKFDAI
  YETEMSKTALQFRTEI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014465
• ORF Size: 888 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014465.2, NP_055280.2
• RefSeq Size: 1334 bp
• RefSeq ORF: 891 bp
• Locus ID: 27284
• UniProt ID: O43704
• Cytogenetics: 4q13.3
• Domains: Sulfotransfer
• Gene Summary: Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. However, the total genomic length of this gene is greater than that of other SULT1 genes. [provided by RefSeq, Jul 2008]