TIPIN (NM_017858) Human Tagged ORF Clone

CAT#: RG201502

TIPIN (tGFP-tagged) - Human TIMELESS interacting protein (TIPIN)

Product Images

Circular map for RG201502

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TIPIN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201502 representing NM_017858
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGAACCACAGGAGAATGGCGTGATTGACCTACCAGATTATGAGCATGTAGAAGATGAAACTTTTC
  CTCCTTTCCCACCTCCAGCCTCTCCAGAGAGACAAGATGGTGAAGGAACTGAGCCTGATGAAGAGTCAGG
  AAATGGAGCACCTGTTCCTGTACCTCCAAAGAGAACAGTTAAAAGAAATATACCCAAGCTGGATGCTCAG
  AGATTAATTTCAGAGAGAGGACTTCCAGCCTTAAGGCATGTATTTGATAAGGCAAAATTCAAAGGTAAAG
  GTCATGAGGCTGAAGACTTGAAGATGCTAATCAGACACATGGAGCACTGGGCACATAGGCTATTCCCTAA
  ACTGCAGTTTGAGGATTTTATTGACAGAGTTGAATACCTGGGAAGTAAAAAGGAAGTTCAGACCTGTTTA
  AAACGAATTCGACTTGATCTCCCTATTTTACATGAAGATTTTGTTAGCAATAATGATGAAGTTGCGGAGA
  ATAATGAACATGATGTCACTTCTACTGAATTAGATCCCTTTCTGACAAACTTATCTGAAAGTGAGATGTT
  TGCTTCTGAGTTAAGTAGAAGCCTAACAGAAGAGCAACAACAAAGAATTGAGAGAAATAAACAACTGGCC
  TTGGAAAGAAGGCAGGCAAAGCTGCTGAGTAATAGTCAGACCCTAGGAAATGATATGTTAATGAATACAC
  CCAGGGCACACACGGTTGAAGAGGTTAATACTGATGAGGATCAAAAGGAGGAGTCAAATGGATTAAACGA
  AGACATTCTGGACAATCCATGTAATGATGCTATTGCCAATACTTTAAATGAAGAGGAAACACTGCTGGAC
  CAGTCTTTTAAAAATGTGCAACAGCAACTTGATGCTACATCCAGAAATATTACTGAAGCTAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201502 representing NM_017858
  Red=Cloning site Green=Tags(s)
  
  MLEPQENGVIDLPDYEHVEDETFPPFPPPASPERQDGEGTEPDEESGNGAPVPVPPKRTVKRNIPKLDAQ
  RLISERGLPALRHVFDKAKFKGKGHEAEDLKMLIRHMEHWAHRLFPKLQFEDFIDRVEYLGSKKEVQTCL
  KRIRLDLPILHEDFVSNNDEVAENNEHDVTSTELDPFLTNLSESEMFASELSRSLTEEQQQRIERNKQLA
  LERRQAKLLSNSQTLGNDMLMNTPRAHTVEEVNTDEDQKEESNGLNEDILDNPCNDAIANTLNEEETLLD
  QSFKNVQQQLDATSRNITEAR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017858
• ORF Size: 903 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017858.2
• RefSeq Size: 1077 bp
• RefSeq ORF: 906 bp
• Locus ID: 54962
• UniProt ID: Q9BVW5
• Cytogenetics: 15q22.31
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is part of the replisome complex, a group of proteins that support DNA replication. It binds TIM, which is involved in circadian rhythm regulation, and aids in protecting cells against DNA damage and stress. Two pseudogenes and two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]