ALG9 (NM_024740) Human Tagged ORF Clone

CAT#: RG201405

ALG9 (tGFP-tagged) - Human asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae) (ALG9), transcript variant 1

Product Images

Circular map for RG201405

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALG9
• Synonyms: CDG1L; DIBD1; GIKANIS; LOH11CR1J
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201405 representing NM_024740
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGTCGAGGGGCTCGGCAGCGCCTGAAGGGCAGCGGGGCCAGCAGTGGGGATACGGCCCCGGCTG
  CGGACAAGCTGCGGGAGCTGCTGGGCAGCCGAGAGGCGGGCGGCGCGGAGCACCGGACCGAGTTATCTGG
  GAACAAAGCAGGACAAGTCTGGGCACCTGAAGGATCTACTGCTTTCAAGTGTCTGCTTTCAGCAAGGTTA
  TGTGCTGCTCTCCTGAGCAACATCTCTGACTGTGATGAAACATTCAACTACTGGGAGCCAACACACTACC
  TCATCTATGGGGAAGGGTTTCAGACTTGGGAATATTCCCCAGCATATGCCATTCGCTCCTATGCTTACCT
  GTTGCTTCATGCCTGGCCAGCTGCATTTCATGCAAGAATTCTACAAACTAATAAGATTCTTGTGTTTTAC
  TTTTTGCGATGTCTTCTGGCTTTTGTGAGCTGTATTTGTGAACTTTACTTTTACAAGGCTGTGTGCAAGA
  AGTTTGGGTTGCACGTGAGTCGAATGATGCTAGCCTTCTTGGTTCTCAGCACTGGCATGTTTTGCTCATC
  ATCAGCATTCCTTCCTAGTAGCTTCTGTATGTACACTACGTTGATAGCCATGACTGGATGGTATATGGAC
  AAGACTTCCATTGCTGTGCTGGGAGTAGCAGCTGGGGCTATCTTAGGCTGGCCATTCAGTGCAGCTCTTG
  GTTTACCCATTGCCTTTGATTTGCTGGTCATGAAACACAGGTGGAAGAGTTTCTTTCATTGGTCGCTGAT
  GGCCCTCATACTATTTCTGGTGCCTGTGGTGGTCATTGACAGCTACTATTATGGGAAGTTGGTGATTGCA
  CCACTCAACATTGTTTTGTATAATGTCTTTACTCCTCATGGACCTGATCTTTATGGTACAGAACCCTGGT
  ATTTCTATTTAATTAATGGATTTCTGAATTTCAATGTAGCCTTTGCTTTGGCTCTCCTAGTCCTACCACT
  GACTTCTCTTATGGAATACCTGCTGCAGAGATTTCATGTTCAGAATTTAGGCCACCCGTATTGGCTTACC
  TTGGCTCCAATGTATATTTGGTTTATAATTTTCTTCATCCAGCCTCACAAAGAGGAGAGATTTCTTTTCC
  CTGTGTATCCACTTATATGTCTCTGTGGCGCTGTGGCTCTCTCTGCACTTCAGCACAGTTTTCTGTACTT
  CCAGAAATGTTACCACTTTGTGTTTCAACGATATCGCCTGGAGCACTATACTGTGACATCGAATTGGCTG
  GCATTAGGAACTGTCTTCCTGTTTGGGCTCTTGTCATTTTCTCGCTCTGTGGCACTGTTCAGAGGATATC
  ACGGGCCCCTTGATTTGTATCCAGAATTTTACCGAATTGCTACAGACCCAACCATCCACACTGTCCCAGA
  AGGCAGACCTGTGAATGTCTGTGTGGGAAAAGAGTGGTATCGATTTCCCAGCAGCTTCCTTCTTCCTGAC
  AATTGGCAGCTTCAGTTCATTCCATCAGAGTTCAGAGGTCAGTTACCAAAACCTTTTGCAGAAGGACCTC
  TGGCCACCCGGATTGTTCCTACTGACATGAATGACCAGAATCTAGAAGAGCCATCCAGATATATTGATAT
  CAGTAAATGCCATTATTTAGTGGATTTGGACACCATGAGAGAAACACCCCGGGAGCCAAAATATTCATCC
  AATAAAGAAGAATGGATCAGCTTGGCCTATAGACCATTCCTTGATGCTTCTAGATCTTCAAAGCTGCTGC
  GGGCATTCTATGTCCCCTTCCTGTCAGATCAGTATACAGTGTACGTAAACTACACCATCCTCAAACCCCG
  GAAAGCAAAGCAAATCAGGAAGAAAAGTGGAGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201405 representing NM_024740
  Red=Cloning site Green=Tags(s)
  
  MASRGARQRLKGSGASSGDTAPAADKLRELLGSREAGGAEHRTELSGNKAGQVWAPEGSTAFKCLLSARL
  CAALLSNISDCDETFNYWEPTHYLIYGEGFQTWEYSPAYAIRSYAYLLLHAWPAAFHARILQTNKILVFY
  FLRCLLAFVSCICELYFYKAVCKKFGLHVSRMMLAFLVLSTGMFCSSSAFLPSSFCMYTTLIAMTGWYMD
  KTSIAVLGVAAGAILGWPFSAALGLPIAFDLLVMKHRWKSFFHWSLMALILFLVPVVVIDSYYYGKLVIA
  PLNIVLYNVFTPHGPDLYGTEPWYFYLINGFLNFNVAFALALLVLPLTSLMEYLLQRFHVQNLGHPYWLT
  LAPMYIWFIIFFIQPHKEERFLFPVYPLICLCGAVALSALQHSFLYFQKCYHFVFQRYRLEHYTVTSNWL
  ALGTVFLFGLLSFSRSVALFRGYHGPLDLYPEFYRIATDPTIHTVPEGRPVNVCVGKEWYRFPSSFLLPD
  NWQLQFIPSEFRGQLPKPFAEGPLATRIVPTDMNDQNLEEPSRYIDISKCHYLVDLDTMRETPREPKYSS
  NKEEWISLAYRPFLDASRSSKLLRAFYVPFLSDQYTVYVNYTILKPRKAKQIRKKSGG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024740
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024740.2, NP_079016.2
• RefSeq Size: 6158 bp
• RefSeq ORF: 1857 bp
• Locus ID: 79796
• UniProt ID: Q9H6U8
• Cytogenetics: 11q23.1
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene encodes an alpha-1,2-mannosyltransferase enzyme that functions in lipid-linked oligosaccharide assembly. Mutations in this gene result in congenital disorder of glycosylation type Il. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]