MEIS2 (NM_002399) Human Tagged ORF Clone

CAT#: RG201395

MEIS2 (tGFP-tagged) - Human Meis homeobox 2 (MEIS2), transcript variant f

Product Images

Circular map for RG201395

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MEIS2
• Synonyms: CPCMR; HsT18361; MRG1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201395 representing NM_002399
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGAGTAGGGGTTCCCGCTTCCATGTACGGAGACCCTCACGCGCCGCGGCCGATCCCCCCGGTTC
  ACCACCTGAACCACGGGCCGCCGCTCCACGCCACACAGCACTACGGCGCGCACGCCCCGCACCCCAATGT
  CATGCCGGCCAGTATGGGATCCGCTGTCAACGACGCCTTGAAGCGGGACAAGGACGCGATCTATGGGCAC
  CCGTTGTTTCCTCTGTTAGCTCTGGTCTTTGAGAAGTGCGAGCTGGCGACCTGCACTCCCCGGGAACCTG
  GAGTGGCTGGCGGAGACGTCTGCTCCTCCGACTCCTTCAACGAGGACATCGCGGTCTTCGCCAAGCAGGT
  TCGCGCCGAAAAGCCACTTTTTTCCTCAAATCCAGAGCTGGACAATTTGATGATACAAGCAATACAAGTA
  CTAAGGTTTCATCTTTTGGAGTTAGAAAAGGTCCACGAACTGTGCGATAACTTCTGCCACCGATACATTA
  GCTGTTTGAAGGGGAAAATGCCCATCGACCTCGTCATTGATGAAAGAGACGGCAGCTCCAAGTCAGATCA
  TGAAGAACTTTCAGGCTCCTCCACAAATCTCGCTGACCATAACCCTTCTTCTTGGCGAGACCACGATGAT
  GCAACCTCAACCCACTCAGCAGGCACCCCAGGGCCCTCCAGTGGGGGCCATGCTTCCCAGAGCGGAGACA
  ACAGCAGTGAGCAAGGGGATGGTTTAGACAACAGTGTAGCTTCACCTGGTACAGGTGACGATGATGATCC
  GGATAAGGACAAAAAACGCCAGAAGAAAAGAGGCATTTTCCCCAAAGTAGCAACAAATATCATGAGAGCA
  TGGCTCTTCCAGCATCTCACACATCCGTACCCTTCCGAAGAGCAGAAGAAACAGTTAGCGCAAGACACAG
  GACTTACAATTCTCCAAGTAAACAACTGGTTTATTAATGCCAGAAGAAGAATAGTACAGCCCATGATTGA
  CCAGTCAAATCGAGCAGTGAGCCAAGGAGCAGCATATAGTCCAGAGGGTCAGCCCATGGGGAGCTTTGTG
  TTGGATGGTCAGCAACACATGGGGATCCGGCCTGCAGGACCTATGAGTGGAATGGGCATGAATATGGGCA
  TGGATGGGCAATGGCACTACATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201395 representing NM_002399
  Red=Cloning site Green=Tags(s)
  
  MDGVGVPASMYGDPHAPRPIPPVHHLNHGPPLHATQHYGAHAPHPNVMPASMGSAVNDALKRDKDAIYGH
  PLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQVRAEKPLFSSNPELDNLMIQAIQV
  LRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDERDGSSKSDHEELSGSSTNLADHNPSSWRDHDD
  ATSTHSAGTPGPSSGGHASQSGDNSSEQGDGLDNSVASPGTGDDDDPDKDKKRQKKRGIFPKVATNIMRA
  WLFQHLTHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVSQGAAYSPEGQPMGSFV
  LDGQQHMGIRPAGPMSGMGMNMGMDGQWHYM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002399
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002399.3
• RefSeq Size: 3110 bp
• RefSeq ORF: 1146 bp
• Locus ID: 4212
• UniProt ID: O14770
• Cytogenetics: 15q14
• Domains: homeobox
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]