CP2c (TFCP2) (NM_005653) Human Tagged ORF Clone

CAT#: RG201221

TFCP2 (tGFP-tagged) - Human transcription factor CP2 (TFCP2), transcript variant 1

Product Images

Circular map for RG201221

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CP2c
• Synonyms: LBP1C; LSF; LSF1D; SEF; TFCP2C
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201221 representing NM_005653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGGGCTCTGAAGCTGCCTCTGGCCGACGAAGTGATTGAATCCGGGTTGGTGCAGGACTTTGATG
  CTAGCCTGTCCGGGATCGGCCAGGAACTGGGTGCTGGTGCCTATAGCATGAGTGATGTCCTTGCATTGCC
  CATTTTTAAGCAAGAAGAGTCGAGTTTGCCTCCTGATAATGAGAATAAAATCCTGCCTTTTCAATATGTG
  CTTTGTGCTGCTACCTCTCCAGCAGTGAAACTCCATGATGAAACCCTAACGTATCTCAATCAAGGACAGT
  CTTATGAAATTCGAATGCTAGACAATAGGAAACTTGGAGAACTTCCAGAAATTAATGGCAAATTGGTGAA
  GAGTATATTCCGTGTGGTGTTCCATGACAGAAGGCTTCAGTACACTGAGCATCAGCAGCTAGAGGGCTGG
  AGGTGGAACCGACCTGGAGACAGAATTCTTGACATAGATATCCCGATGTCTGTGGGTATAATCGATCCTA
  GGGCTAATCCAACTCAACTAAATACAGTGGAGTTCCTGTGGGACCCTGCAAAGAGGACATCTGTGTTTAT
  TCAGGTGCACTGTATTAGCACAGAGTTCACTATGAGGAAACATGGTGGAGAAAAGGGGGTGCCATTCCGA
  GTACAAATAGATACCTTCAAGGAGAATGAAAACGGGGAATATACTGAGCACTTACACTCGGCCAGCTGCC
  AGATCAAAGTTTTCAAGCCCAAAGGTGCAGACAGAAAGCAAAAAACGGATAGGGAAAAAATGGAGAAACG
  AACACCTCATGAAAAGGAGAAATATCAGCCTTCCTATGAGACAACCATACTCACAGAGTGTTCTCCATGG
  CCCGAGATCACGTATGTCAATAACTCCCCATCACCTGGCTTCAACAGTTCCCATAGCAGTTTTTCTCTTG
  GGGAAGGAAATGGTTCACCAAACCACCAGCCAGAGCCACCCCCTCCAGTCACAGATAACCTCTTACCAAC
  AACCACACCTCAGGAAGCTCAGCAGTGGTTGCATCGAAATCGTTTTTCTACATTCACAAGGCTTTTCACA
  AACTTCTCAGGGGCAGATTTATTGAAATTAACTAGAGATGATGTGATCCAAATCTGTGGCCCTGCAGATG
  GAATCAGACTTTTTAATGCATTAAAAGGCCGGATGGTGCGTCCAAGGTTAACCATTTATGTTTGTCAGGA
  ATCACTGCAGTTGAGGGAGCAGCAACAACAGCAGCAGCAACAGCAGCAGAAGCATGAGGATGGAGACTCA
  AATGGTACTTTCTTCGTTTACCATGCTATCTATCTAGAAGAACTAACAGCTGTTGAATTGACAGAAAAAA
  TTGCTCAGCTTTTCAGCATTTCCCCTTGCCAGATCAGCCAGATTTACAAGCAGGGGCCAACAGGAATTCA
  TGTGCTCATCAGTGATGAGATGATACAGAACTTTCAGGAAGAAGCATGTTTTATTCTGGACACAATGAAA
  GCAGAAACCAATGATAGCTATCATATCATACTGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201221 representing NM_005653
  Red=Cloning site Green=Tags(s)
  
  MAWALKLPLADEVIESGLVQDFDASLSGIGQELGAGAYSMSDVLALPIFKQEESSLPPDNENKILPFQYV
  LCAATSPAVKLHDETLTYLNQGQSYEIRMLDNRKLGELPEINGKLVKSIFRVVFHDRRLQYTEHQQLEGW
  RWNRPGDRILDIDIPMSVGIIDPRANPTQLNTVEFLWDPAKRTSVFIQVHCISTEFTMRKHGGEKGVPFR
  VQIDTFKENENGEYTEHLHSASCQIKVFKPKGADRKQKTDREKMEKRTPHEKEKYQPSYETTILTECSPW
  PEITYVNNSPSPGFNSSHSSFSLGEGNGSPNHQPEPPPPVTDNLLPTTTPQEAQQWLHRNRFSTFTRLFT
  NFSGADLLKLTRDDVIQICGPADGIRLFNALKGRMVRPRLTIYVCQESLQLREQQQQQQQQQQKHEDGDS
  NGTFFVYHAIYLEELTAVELTEKIAQLFSISPCQISQIYKQGPTGIHVLISDEMIQNFQEEACFILDTMK
  AETNDSYHIILK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005653
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005653.5
• RefSeq Size: 2393 bp
• RefSeq ORF: 1509 bp
• Locus ID: 7024
• UniProt ID: Q12800
• Cytogenetics: 12q13.12-q13.13
• Domains: CP2
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a transcription factor that binds the alpha-globin promoter and activates transcription of the alpha-globin gene. The encoded protein regulates erythroid gene expression, plays a role in the transcriptional switch of globin gene promoters, and it activates many other cellular and viral gene promoters. The gene product interacts with certain inflammatory response factors, and polymorphisms of this gene may be involved in the pathogenesis of Alzheimer's disease. [provided by RefSeq, Mar 2010]