MCCC1 (NM_020166) Human Tagged ORF Clone

CAT#: RG200909

MCCC1 (tGFP-tagged) - Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RG200909

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MCCC1
• Synonyms: MCC-B; MCCA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200909 representing NM_020166
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCCTCTGCGGTGTCGGTGCTGCTGGTGGCGGCGGAGAGGAACCGGTGGCATCGTCTCCCGA
  GCCTGCTCCTGCCGCCGAGGACATGGGTGTGGAGGCAAAGAACCATGAAGTACACAACAGCCACAGGAAG
  AAACATTACCAAGGTCCTCATTGCAAACAGAGGAGAAATTGCCTGCAGGGTGATGCGCACAGCCAAAAAA
  CTGGGTGTACAGACTGTGGCGGTTTATAGTGAGGCTGACAGAAATTCCATGCATGTAGATATGGCAGATG
  AAGCATATTCCATCGGCCCCGCTCCCTCCCAGCAGAGCTACCTATCTATGGAGAAAATCATTCAAGTGGC
  CAAGACCTCTGCTGCACAGGCTATCCATCCAGGATGCGGTTTTCTTTCAGAAAACATGGAATTTGCTGAA
  CTTTGTAAGCAAGAAGGAATTATTTTTATAGGCCCTCCTCCATCTGCAATTAGAGACATGGGTATAAAGA
  GCACATCCAAATCCATAATGGCTGCTGCTGGAGTACCTGTTGTGGAGGGTTATCATGGTGAGGACCAATC
  AGACCAGTGCCTGAAGGAACACGCCAGGAGAATTGGCTATCCTGTCATGATTAAAGCCGTCCGGGGTGGA
  GGAGGAAAAGGAATGAGGATTGTTAGATCAGAACAAGAATTTCAAGAACAGTTAGAGTCAGCACGGAGAG
  AAGCTAAGAAGTCTTTCAATGATGATGCTATGCTGATCGAGAAGTTTGTAGACACACCGAGGCATGTAGA
  AGTCCAGGTGTTTGGTGATCACCATGGCAATGCTGTGTACTTGTTTGAAAGAGACTGTAGTGTGCAGAGG
  CGACATCAGAAGATCATTGAGGAGGCCCCAGCGCCTGGTATTAAATCTGAAGTAAGAAAAAAGCTGGGAG
  AAGCTGCAGTCAGAGCTGCTAAAGCTGTAAATTATGTTGGAGCAGGGACTGTGGAGTTTATTATGGACTC
  AAAACATAATTTCTGTTTCATGGAGATGAATACAAGGCTGCAAGTGGAACATCCTGTTACTGAGATGATC
  ACAGGAACTGACTTGGTGGAGTGGCAGCTTAGAATTGCAGCAGGAGAGAAGATTCCTTTGAGCCAGGAAG
  AAATAACTCTGCAGGGCCATGCCTTCGAAGCTAGAATATATGCAGAAGATCCTAGCAATAACTTCATGCC
  TGTGGCAGGCCCATTAGTGCACCTCTCTACTCCTCGAGCAGACCCTTCCACCAGGATTGAAACTGGAGTA
  CGGCAAGGAGACGAAGTTTCCGTGCATTATGACCCCATGATTGCGAAGCTGGTCGTGTGGGCAGCAGATC
  GCCAGGCGGCATTGACAAAACTGAGGTACAGCCTTCGTCAGTACAATATTGTTGGACTGCCCACCAACAT
  TGACTTCTTACTCAACCTGTCTGGCCACCCAGAGTTTGAAGCTGGGAACGTGCACACTGATTTCATCCCT
  CAACACCACAAACAGTTGTTGCTCAGTCGGAAGGCTGCAGCCAAAGAGTCTTTATGCCAGGCAGCCCTGG
  GTCTCATCCTCAAGGAGAAAGCCATGACCGACACTTTCACTCTTCAGGCACATGATCAATTCTCTCCATT
  TTCGTCTAGCAGTGGAAGAAGACTGAATATCTCGTATACCAGAAACATGACTCTTAAAGATGGTAAAAAC
  AATGTAGCCATAGCTGTAACGTATAACCATGATGGGTCTTATAGCATGCAGATTGAAGATAAAACTTTCC
  AAGTCCTTGGTAATCTTTACAGCGAGGGAGACTGCACTTACCTGAAATGTTCTGTTAATGGAGTTGCTAG
  TAAAGCGAAGCTGATTATCCTGGAAAACACTATTTACCTATTTTCCAAGGAAGGAAGTATTGAGATTGAC
  ATTCCAGTCCCCAAATACTTATCTTCTGTGAGCTCACAAGAAACTCAGGGCGGCCCCTTAGCTCCTATGA
  CTGGAACCATTGAAAAGGTGTTTGTCAAAGCTGGAGACAAAGTGAAAGCGGGAGATTCCCTCATGGTTAT
  GATCGCCATGAAGATGGAGCATACCATAAAGTCTCCAAAGGATGGCACAGTAAAGAAAGTGTTCTACAGA
  GAAGGTGCTCAGGCCAACAGACACACTCCTTTAGTCGAGTTTGAGGAGGAAGAATCAGACAAAAGGGAAT
  CGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200909 representing NM_020166
  Red=Cloning site Green=Tags(s)
  
  MAAASAVSVLLVAAERNRWHRLPSLLLPPRTWVWRQRTMKYTTATGRNITKVLIANRGEIACRVMRTAKK
  LGVQTVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLSMEKIIQVAKTSAAQAIHPGCGFLSENMEFAE
  LCKQEGIIFIGPPPSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARRIGYPVMIKAVRGG
  GGKGMRIVRSEQEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQR
  RHQKIIEEAPAPGIKSEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFCFMEMNTRLQVEHPVTEMI
  TGTDLVEWQLRIAAGEKIPLSQEEITLQGHAFEARIYAEDPSNNFMPVAGPLVHLSTPRADPSTRIETGV
  RQGDEVSVHYDPMIAKLVVWAADRQAALTKLRYSLRQYNIVGLPTNIDFLLNLSGHPEFEAGNVHTDFIP
  QHHKQLLLSRKAAAKESLCQAALGLILKEKAMTDTFTLQAHDQFSPFSSSSGRRLNISYTRNMTLKDGKN
  NVAIAVTYNHDGSYSMQIEDKTFQVLGNLYSEGDCTYLKCSVNGVASKAKLIILENTIYLFSKEGSIEID
  IPVPKYLSSVSSQETQGGPLAPMTGTIEKVFVKAGDKVKAGDSLMVMIAMKMEHTIKSPKDGTVKKVFYR
  EGAQANRHTPLVEFEEEESDKRESE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020166
• ORF Size: 2175 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020166.2, NP_064551.2
• RefSeq Size: 2551 bp
• RefSeq ORF: 2178 bp
• Locus ID: 56922
• UniProt ID: Q96RQ3
• Cytogenetics: 3q27.1
• Domains: biotin_lipoyl, CPSase_L_D2, CPSase_L_chain, Biotin_carb_C
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq, Jul 2008]