DCUN1D5 (NM_032299) Human Tagged ORF Clone

CAT#: RG200870

DCUN1D5 (tGFP-tagged) - Human DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (DCUN1D5)

Product Images

Circular map for RG200870

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DCUN1D5
• Synonyms: SCCRO5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200870 representing NM_032299
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGAAGAAGAAGAGAAAATCCCCTGGGGTGGCAGCAGCAGTAGCGGAAGACGGAGGCCTCAAAA
  AGTGTAAAATCTCCAGCTATTGCAGATCCCAACCCCCTGCTAGACTAATAAGTGGAGAGGAACATTTTTC
  AAGCAAGAAGTGCCTGGCTTGGTTTTATGAATATGCAGGTCCTGATGAAGTTGTAGGGCCAGAAGGAATG
  GAAAAATTTTGTGAAGACATTGGTGTTGAACCTGAAAATATTATTATGTTAGTTTTAGCGTGGAAATTGG
  AGGCTGAAAGCATGGGATTTTTTACCAAGGAAGAATGGTTAAAGGGAATGACTTCATTACAGTGTGACTG
  CACAGAAAAGTTACAAAACAAATTTGACTTTTTGCGCTCACAGTTGAATGATATTTCGTCATTTAAGAAT
  ATCTACAGATATGCCTTTGATTTTGCAAGGGATAAAGATCAGAGAAGCCTTGATATTGATACTGCTAAAT
  CTATGTTAGCTCTTCTGCTTGGGAGGACATGGCCACTGTTTTCAGTATTTTACCAGTACCTGGAGCAATC
  AAAGTATCGTGTTATGAACAAAGATCAATGGTACAATGTATTAGAATTCAGCAGAACAGTCCATGCTGAT
  CTTAGTAACTATGATGAAGATGGTGCTTGGCCTGTTCTTCTTGATGAATTTGTTGAGTGGCAAAAAGTCC
  GTCAGACATCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200870 representing NM_032299
  Red=Cloning site Green=Tags(s)
  
  MPVKKKRKSPGVAAAVAEDGGLKKCKISSYCRSQPPARLISGEEHFSSKKCLAWFYEYAGPDEVVGPEGM
  EKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSLQCDCTEKLQNKFDFLRSQLNDISSFKN
  IYRYAFDFARDKDQRSLDIDTAKSMLALLLGRTWPLFSVFYQYLEQSKYRVMNKDQWYNVLEFSRTVHAD
  LSNYDEDGAWPVLLDEFVEWQKVRQTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032299
• ORF Size: 711 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032299.4
• RefSeq Size: 1277 bp
• RefSeq ORF: 714 bp
• Locus ID: 84259
• UniProt ID: Q9BTE7
• Cytogenetics: 11q22.3
• Domains: DUF298
• Gene Summary: Contributes to the neddylation of all cullins by transfering NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which is necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs) (PubMed:26906416, PubMed:23201271, PubMed:19617556). May play a role in DNA damage response and may participate to cell proliferation and anchorage-independent cell growth (PubMed:23098533, PubMed:24192928).[UniProtKB/Swiss-Prot Function]