FARSLA (FARSA) (NM_004461) Human Tagged ORF Clone

CAT#: RG200365

FARSA (tGFP-tagged) - Human phenylalanyl-tRNA synthetase, alpha subunit (FARSA)

Product Images

Circular map for RG200365

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FARSLA
• Synonyms: CML33; FARSL; FARSLA; FRSA; PheHA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200365 representing NM_004461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGGTCAGGTGGCGGAACTGCTGCTCCGGCGGCTGGAGGCGTCTGATGGCGGCCTGGACAGCG
  CCGAGTTGGCGGCTGAGCTGGGCATGGAGCACCAGGCGGTGGTGGGCGCCGTGAAGAGCCTTCAGGCGCT
  GGGCGAGGTCATCGAGGCTGAACTTCGGTCCACCAAGCACTGGGAGCTTACTGCGGAGGGCGAGGAGATT
  GCCCGGGAGGGCAGCCATGAGGCCCGTGTGTTTCGAAGCATTCCCCCAGAGGGCCTGGCCCAGAGCGAGC
  TTATGCGACTGCCCAGTGGCAAAGTGGGCTTCAGCAAGGCCATGTCCAACAAGTGGATTCGGGTGGACAA
  GAGTGCGGCTGACGGGCCCCGGGTGTTCCGAGTGGTGGACAGCATGGAGGATGAGGTGCAGCGGCGGCTC
  CAGCTGGTCCGGGGGGGACAGGCTGAGAAGCTGGGGGAGAAGGAGAGGAGCGAGCTGAGGAAGAGGAAGC
  TGTTGGCTGAAGTGACTCTGAAGACCTACTGGGTGAGCAAAGGCAGTGCCTTTAGTACCAGCATCTCCAA
  GCAAGAGACAGAGCTGAGCCCAGAGATGATCTCCAGTGGCTCTTGGCGGGACCGGCCCTTCAAGCCCTAC
  AACTTCTTGGCCCACGGTGTCCTCCCCGACAGCGGCCACCTTCACCCGCTGCTCAAGGTCCGCTCCCAGT
  TCCGACAGATCTTCCTGGAGATGGGGTTCACCGAGATGCCGACTGATAACTTCATTGAGAGCTCCTTCTG
  GAACTTTGACGCCCTCTTCCAGCCCCAGCAGCACCCAGCCCGTGACCAGCACGACACCTTCTTCCTTCGA
  GATCCAGCGGAGGCCCTGCAGCTCCCAATGGACTATGTCCAGCGGGTCAAGCGGACCCACTCTCAGGGCG
  GCTACGGCTCACAGGGGTACAAGTATAACTGGAAGCTGGACGAGGCCCGGAAAAACCTACTGCGAACCCA
  CACCACATCAGCCAGCGCCCGTGCGCTCTACCGCCTTGCCCAGAAGAAGCCCTTCACTCCGGTCAAGTAC
  TTCTCCATCGACCGCGTATTCCGGAATGAGACCCTGGACGCCACGCACCTGGCTGAGTTCCACCAGATCG
  AGGGCGTGGTGGCGGATCATGGTCTCACCTTGGGCCACCTCATGGGCGTTCTGCGGGAGTTCTTCACCAA
  GCTGGGTATCACGCAACTCCGCTTCAAGCCAGCCTACAACCCATACACAGAGCCCAGCATGGAGGTGTTC
  AGCTACCACCAAGGCCTGAAGAAGTGGGTGGAGGTCGGAAACTCGGGGGTCTTCCGTCCAGAGATGCTGC
  TGCCCATGGGGCTTCCCGAGAACGTGTCGGTCATTGCCTGGGGCCTCTCCCTGGAGCGCCCAACGATGAT
  CAAATATGGCATCAACAATATCCGGGAGCTGGTGGGCCACAAGGTGAACCTGCAGATGGTGTATGACAGT
  CCCCTGTGCCGCCTGGATGCCGAGCCGAGGCCCCCTCCCACACAGGAGGCTGCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200365 representing NM_004461
  Red=Cloning site Green=Tags(s)
  
  MADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEI
  AREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRL
  QLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPY
  NFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLR
  DPAEALQLPMDYVQRVKRTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKY
  FSIDRVFRNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSMEVF
  SYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIRELVGHKVNLQMVYDS
  PLCRLDAEPRPPPTQEAA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004461
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004461.3
• RefSeq Size: 1853 bp
• RefSeq ORF: 1527 bp
• Locus ID: 2193
• UniProt ID: Q9Y285
• Cytogenetics: 19p13.13
• Domains: tRNA-synt_2d
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq, Jul 2008]