N acetylglucosamine kinase (NAGK) (NM_017567) Human Tagged ORF Clone
CAT#: RG200113
- TrueORF®
NAGK (tGFP-tagged) - Human N-acetylglucosamine kinase (NAGK)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_017567" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | N acetylglucosamine kinase |
Synonyms | GNK; HSA242910 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200113 representing NM_017567
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGATCTATGGGGGTGTAGAGGGGGGAGGCACACGATCCGAGGTCCTTTTAGTCTCAGAGGATG GGAAGATCCTGGCAGAAGCAGATGGACTGAGCACAAACCACTGGCTGATCGGGACAGACAAGTGTGTGGA GAGGATCAATGAGATGGTGAACAGGGCCAAACGGAAAGCAGGGGTGGATCCTCTGGTACCGCTGCGAAGC TTGGGCCTATCTCTGAGCGGTGGGGACCAGGAGGACGCGGGGAGGATCCTGATCGAGGAGCTCAGGGACC GATTTCCCTACCTGAGTGAAAGCTACTTAATCACCACCGATGCCGCCGGCTCCATCGCCACAGCTACACC GGATGGTGGAGTTGTGCTCATATCTGGAACAGGCTCCAACTGCAGGCTCATCAACCCTGATGGCTCCGAG AGTGGCTGCGGCGGCTGGGGCCATATGATGGGTGATGAGGGTTCAGCCTACTGGATCGCACACCAAGCAG TGAAAATAGTGTTTGACTCCATTGACAACCTAGAGGCGGCTCCTCATGATATCGGCTACGTCAAACAGGC CATGTTCCACTATTTCCAGGTGCCAGATCGGCTAGGGATACTCACTCACCTGTATAGGGACTTTGATAAA TGCAGGTTTGCTGGGTTTTGCCGGAAAATTGCAGAAGGTGCTCAGCAGGGAGACCCCCTTTCCCGCTATA TCTTCAGGAAGGCTGGGGAGATGCTGGGCAGACACATCGTAGCAGTGTTGCCCGAGATTGACCCGGTCTT GTTCCAGGGCAAGATTGGACTCCCCATCCTGTGCGTGGGCTCTGTGTGGAAGAGCTGGGAGCTGCTGAAG GAAGGTTTTCTTCTGGCGCTGACCCAGGGCAGAGAGATCCAGGCTCAGAACTTCTTCTCCAGCTTCACCC TGATGAAGCTGAGGCACTCCTCCGCTCTGGGTGGGGCCAGCCTAGGGGCCAGGCACATCGGGCACCTCCT CCCCATGGACTATAGCGCCAATGCCATTGCCTTCTATTCCTACACCTTTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200113 representing NM_017567
Red=Cloning site Green=Tags(s) MAAIYGGVEGGGTRSEVLLVSEDGKILAEADGLSTNHWLIGTDKCVERINEMVNRAKRKAGVDPLVPLRS LGLSLSGGDQEDAGRILIEELRDRFPYLSESYLITTDAAGSIATATPDGGVVLISGTGSNCRLINPDGSE SGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNLEAAPHDIGYVKQAMFHYFQVPDRLGILTHLYRDFDK CRFAGFCRKIAEGAQQGDPLSRYIFRKAGEMLGRHIVAVLPEIDPVLFQGKIGLPILCVGSVWKSWELLK EGFLLALTQGREIQAQNFFSSFTLMKLRHSSALGGASLGARHIGHLLPMDYSANAIAFYSYTFS TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_017567 |
ORF Size | 1032 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017567.2, NP_060037.2 |
RefSeq Size | 1535 bp |
RefSeq ORF | 1035 bp |
Locus ID | 55577 |
UniProt ID | Q9UJ70 |
Cytogenetics | 2p13.3 |
Domains | BcrAD_BadFG |
Protein Families | Druggable Genome |
Protein Pathways | Amino sugar and nucleotide sugar metabolism |
Gene Summary | This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars. [provided by RefSeq, Nov 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200113 | NAGK (Myc-DDK-tagged)-Human N-acetylglucosamine kinase (NAGK) |
USD 457.00 |
|
RC200113L3 | Lenti ORF clone of Human N-acetylglucosamine kinase (NAGK), Myc-DDK-tagged |
USD 757.00 |
|
RC200113L4 | Lenti ORF clone of Human N-acetylglucosamine kinase (NAGK), mGFP tagged |
USD 757.00 |
|
SC114077 | NAGK (untagged)-Human N-acetylglucosamine kinase (NAGK) |
USD 457.00 |
|
SC320640 | NAGK (untagged)-Human N-acetylglucosamine kinase (NAGK) |
USD 457.00 |
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