NARS1 (NM_004539) Human Tagged ORF Clone

CAT#: RG200087

NARS (tGFP-tagged) - Human asparaginyl-tRNA synthetase (NARS)

Product Images

Circular map for RG200087

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NARS1
• Synonyms: ASNRS; NARS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200087 representing NM_004539
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTAGCAGAGCTGTACGTCTCTGACCGAGAGGGAAGCGATGCCACGGGAGATGGAACCAAGGAGA
  AACCATTTAAAACAGGTCTAAAGGCTTTGATGACAGTAGGGAAAGAACCATTTCCTACCATTTACGTAGA
  TTCACAAAAAGAAAATGAGAGGTGGAATGTTATTTCTAAATCACAGTTGAAGAACATTAAAAAGATGTGG
  CATAGGGAACAAATGAAGAGTGAATCCCGGGAAAAGAAAGAGGCAGAAGATAGTTTACGAAGAGAAAAGA
  ACCTGGAAGAAGCAAAGAAGATTACCATTAAAAATGATCCAAGTCTCCCAGAGCCAAAATGTGTGAAGAT
  TGGTGCGTTAGAAGGATATAGAGGCCAAAGAGTAAAGGTGTTTGGCTGGGTCCACAGGCTGCGCAGGCAA
  GGAAAGAATTTAATGTTTCTGGTGTTGCGAGATGGTACAGGTTATCTTCAGTGTGTCTTGGCGGATGAGT
  TGTGTCAGTGCTACAATGGAGTTCTCTTGTCCACGGAGAGCAGTGTTGCAGTGTATGGAATGCTAAATCT
  TACCCCAAAGGGCAAGCAGGCTCCAGGTGGCCATGAGCTGAGTTGTGACTTCTGGGAACTAATTGGGTTG
  GCCCCTGCTGGAGGAGCTGACAACCTGATCAATGAGGAGTCTGACGTTGATGTCCAGCTCAACAACAGAC
  ACATGATGATCCGAGGAGAAAACATGTCCAAAATCCTAAAAGCACGATCCATGGTCACCAGGTGCTTTAG
  AGATCACTTCTTTGATAGGGGGTACTATGAAGTTACTCCTCCAACATTAGTGCAAACACAAGTAGAAGGT
  GGTGCCACACTCTTCAAGCTTGACTATTTTGGGGAAGAGGCATTTTTGACTCAATCCTCTCAGTTGTACT
  TGGAGACCTGCCTCCCAGCCCTGGGAGATGTTTTTTGTATTGCTCAGTCATACCGGGCAGAGCAGTCCAG
  AACACGAAGGCACCTGGCTGAGTACACTCACGTGGAAGCTGAGTGTCCTTTCCTGACTTTTGACGACCTC
  CTGAACCGGTTGGAGGACTTGGTTTGTGATGTGGTAGATCGAATATTGAAGTCACCTGCAGGGAGCATAG
  TGCATGAGCTCAACCCGAACTTTCAGCCCCCCAAACGGCCTTTCAAACGGATGAACTATTCAGATGCTAT
  CGTTTGGCTAAAAGAACATGATGTAAAGAAAGAAGATGGAACTTTCTATGAATTTGGAGAAGATATCCCA
  GAAGCTCCTGAGAGACTGATGACAGACACCATTAATGAACCAATCTTGCTGTGTCGATTTCCTGTGGAGA
  TCAAGTCCTTCTACATGCAGCGATGTCCTGAGGATTCCCGTCTTACTGAATCTGTCGACGTGTTGATGCC
  CAATGTTGGTGAGATTGTGGGAGGCTCAATGCGTATCTTTGATAGTGAAGAAATACTGGCAGGTTATAAA
  AGGGAAGGGATTGACCCCACTCCCTATTACTGGTATACGGATCAGAGAAAATACGGTACATGTCCCCATG
  GAGGATATGGCTTGGGCTTGGAACGATTCTTAACGTGGATTCTGAATAGGTATCACATCCGAGACGTGTG
  CTTATACCCTCGATTTGTCCAGCGTTGCACGCCA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200087 representing NM_004539
  Red=Cloning site Green=Tags(s)
  
  MVLAELYVSDREGSDATGDGTKEKPFKTGLKALMTVGKEPFPTIYVDSQKENERWNVISKSQLKNIKKMW
  HREQMKSESREKKEAEDSLRREKNLEEAKKITIKNDPSLPEPKCVKIGALEGYRGQRVKVFGWVHRLRRQ
  GKNLMFLVLRDGTGYLQCVLADELCQCYNGVLLSTESSVAVYGMLNLTPKGKQAPGGHELSCDFWELIGL
  APAGGADNLINEESDVDVQLNNRHMMIRGENMSKILKARSMVTRCFRDHFFDRGYYEVTPPTLVQTQVEG
  GATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRRHLAEYTHVEAECPFLTFDDL
  LNRLEDLVCDVVDRILKSPAGSIVHELNPNFQPPKRPFKRMNYSDAIVWLKEHDVKKEDGTFYEFGEDIP
  EAPERLMTDTINEPILLCRFPVEIKSFYMQRCPEDSRLTESVDVLMPNVGEIVGGSMRIFDSEEILAGYK
  REGIDPTPYYWYTDQRKYGTCPHGGYGLGLERFLTWILNRYHIRDVCLYPRFVQRCTP
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004539
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004539.4
• RefSeq Size: 2714 bp
• RefSeq ORF: 1647 bp
• Locus ID: 4677
• UniProt ID: O43776
• Cytogenetics: 18q21.31
• Domains: tRNA-synt_2, tRNA_anti
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. [provided by RefSeq, Jul 2008]