TM7SF3 (NM_016551) Human Tagged ORF Clone

CAT#: RG200084

TM7SF3 (tGFP-tagged) - Human transmembrane 7 superfamily member 3 (TM7SF3)

Product Images

Circular map for RG200084

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TM7SF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200084 representing NM_016551
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTCCTGCAGCTGCTGGTCGTAGCGGTGCTGGCATCCGAACACCGGGTGGCTGGTGCAGCCGAGG
  TCTTCGGGAATTCCAGCGAGGGTCTTATTGAATTTTCTGTGGGGAAATTTAGATACTTCGAGCTCAATAG
  GCCCTTTCCAGAGGAAGCTATTTTGCATGATATTTCAAGCAATGTGACTTTTCTTATTTTCCAAATACAC
  TCACAGTATCAGAATACAACTGTTTCCTTTTCTCCGACTCTCCTTTCCAATTCCTCGGAAACAGGCACTG
  CCAGTGGACTGGTTTTCATCCTTAGACCAGAGCAGAGTACATGCACTTGGTACTTGGGGACTTCAGGCAT
  ACAGCCTGTCCAGAATATGGCTATCCTACTCTCCTACTCAGAAAGAGATCCTGTCCCTGGAGGCTGTAAT
  TTGGAGTTCGATTTAGATATTGATCCCAACATTTACTTGGAGTATAATTTCTTTGAAACGACTATCAAGT
  TTGCCCCAGCAAACCTAGGCTATGCGAGAGGCGTAGATCCCCCACCATGTGACGCTGGGACAGACCAGGA
  CTCCAGGTGGAGGTTGCAGTATGATGTCTATCAGTATTTTCTGCCTGAGAATGACCTCACTGAGGAGATG
  TTGCTGAAGCATCTGCAGAGGATGGTCAGTGTGCCCCAGGTGAAGGCCAGTGCTCTCAAGGTGGTTACCC
  TAACAGCTAATGATAAGACAAGTGTTTCCTTCTCCTCCCTCCCGGGACAAGGTGTCATATACAATGTCAT
  TGTTTGGGACCCGTTTCTAAATACATCTGCTGCCTACATTCCTGCTCACACATACGCTTGCAGCTTTGAG
  GCAGGAGAGGGTAGTTGTGCTTCCCTAGGAAGAGTGTCTTCCAAAGTGTTCTTCACTCTTTTTGCCCTGC
  TTGGTTTCTTCATTTGTTTCTTTGGACACAGATTCTGGAAAACAGAATTATTCTTCATAGGCTTTATCAT
  CATGGGATTCTTCTTTTATATACTGATTACAAGACTGACACCTATCAAGTATGATGTGAATCTGATTCTG
  ACAGCTGTCACTGGAAGCGTCGGTGGAATGTTCTTGGTAGCTGTGTGGTGGCGATTTGGAATCCTCTCGA
  TCTGCATGCTCTGTGTTGGACTAGTGCTGGGGTTCCTCATCTCGTCAGTGACTTTCTTTACTCCACTGGG
  AAACCTAAAGATTTTTCATGATGATGGTGTATTCTGGGTCACTTTCTCTTGCATAGCTATCCTCATTCCA
  GTAGTTTTCATGGGCTGCCTAAGAATACTGAACATACTGACTTGTGGAGTCATTGGCTCCTATTCGGTGG
  TTTTAGCCATTGACAGTTACTGGTCCACAAGCCTTTCCTACATCACTTTGAACGTACTCAAGAGAGCGCT
  CAACAAGGATTTCCACAGAGCTTTCACAAATGTGCCTTTTCAAACTAATGACTTCATTATCCTGGCAGTA
  TGGGGCATGCTGGCTGTAAGTGGAATTACGTTACAGATTCGAAGAGAGAGAGGACGACCGTTCTTCCCTC
  CCCACCCATACAAGTTATGGAAGCAAGAGAGAGAGCGCCGAGTGACAAACATTCTGGACCCTAGCTACCA
  CATTCCTCCATTGAGAGAGAGGCTCTATGGCCGATTAACCCAGATTAAAGGGCTCTTCCAGAAGGAGCAG
  CCAGCTGGAGAGAGAACGCCTTTGCTTCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200084 representing NM_016551
  Red=Cloning site Green=Tags(s)
  
  MGFLQLLVVAVLASEHRVAGAAEVFGNSSEGLIEFSVGKFRYFELNRPFPEEAILHDISSNVTFLIFQIH
  SQYQNTTVSFSPTLLSNSSETGTASGLVFILRPEQSTCTWYLGTSGIQPVQNMAILLSYSERDPVPGGCN
  LEFDLDIDPNIYLEYNFFETTIKFAPANLGYARGVDPPPCDAGTDQDSRWRLQYDVYQYFLPENDLTEEM
  LLKHLQRMVSVPQVKASALKVVTLTANDKTSVSFSSLPGQGVIYNVIVWDPFLNTSAAYIPAHTYACSFE
  AGEGSCASLGRVSSKVFFTLFALLGFFICFFGHRFWKTELFFIGFIIMGFFFYILITRLTPIKYDVNLIL
  TAVTGSVGGMFLVAVWWRFGILSICMLCVGLVLGFLISSVTFFTPLGNLKIFHDDGVFWVTFSCIAILIP
  VVFMGCLRILNILTCGVIGSYSVVLAIDSYWSTSLSYITLNVLKRALNKDFHRAFTNVPFQTNDFIILAV
  WGMLAVSGITLQIRRERGRPFFPPHPYKLWKQERERRVTNILDPSYHIPPLRERLYGRLTQIKGLFQKEQ
  PAGERTPLLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016551
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016551.3
• RefSeq Size: 4346 bp
• RefSeq ORF: 1713 bp
• Locus ID: 51768
• UniProt ID: Q9NS93
• Cytogenetics: 12p11.23
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Involved in the inhibition of cytokine-induced death of pancreatic beta cells. Involved in the promotion of insulin secretion from pancreatic beta cells (PubMed:21853325). Is a downstream transcriptional target of p53/TP53, and acts as a pro-survival homeostatic factor that attenuates the development of cellular stress. Maintains protein homeostasis and promotes cell survival through attenuation of endoplasmic reticulum (ER) stress and the subsequent induction of unfolded protein response (UPR) (PubMed:27740623).[UniProtKB/Swiss-Prot Function]