PPIL2 (NM_014337) Human Tagged ORF Clone

CAT#: RG200057

PPIL2 (tGFP-tagged) - Human peptidylprolyl isomerase (cyclophilin)-like 2 (PPIL2), transcript variant 1

Product Images

Circular map for RG200057

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPIL2
• Synonyms: CYC4; Cyp-60; CYP60; hCyP-60; UBOX7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200057 representing NM_014337
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCACTTTTATGGTG
  GCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGACCACTGCAGTCTCTCTCTGCA
  GCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTCTTTGACTTACTGAACATTGTTCCATGGCTT
  AAGAAGTACGGGACCAACCCCAGCAATGGAGAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTT
  CCAAGAACAGTGAGGGGAAGTACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGT
  GGCTGTGAGGACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
  TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGACCCCACCAATT
  TGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAAATAATAGACCCAGATGAAGA
  GAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACAAATGCCGAGACCCGAGAGACCCTGCAGGAG
  CTCTACAAGGAGTTCAAAGGGGACGAGATTCTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGG
  ACAAGCTGAACGCTGCCCACTATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCC
  GGAGACCACACATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
  TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACACCAAAAACCT
  GCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATCTTCCACAGATCCATCCGGAA
  CTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACGGGTGGGGAGTCATACTGGGGGAAGCCCTTC
  AAAGACGAGTTCCGGCCCAACCTCTCGCACACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCA
  ACAGCAACAGGTCTCAATTCTTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTT
  TGGACGGGTTGTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
  CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAGGCCGATGCCC
  AGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAAGTGAAGAGCAGCCAGCCCCA
  GGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTGGGCAAGTACATCAACCCAGCAGCCACGAAG
  CGAGCAGCAGAGGAAGAGCCCTCAACCAGTGCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTT
  TTGGGGACTTCAGCTCCTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200057 representing NM_014337
  Red=Cloning site Green=Tags(s)
  
  MGKRQHQKDKMYITCAEYTHFYGGKKPDLPQTNFRRLPFDHCSLSLQPFVYPVCTPDGIVFDLLNIVPWL
  KKYGTNPSNGEKLDGRSLIKLNFSKNSEGKYHCPVLFTVFTNNTHIVAVRTTGNVYAYEAVEQLNIKAKN
  FRDLLTDEPFSRQDIITLQDPTNLDKFNVSNFYHVKNNMKIIDPDEEKAKQDPSYYLKNTNAETRETLQE
  LYKEFKGDEILAATMKAPEKKKVDKLNAAHYSTGKVSASFTSTAMVPETTHEAAAIDEDVLRYQFVKKKG
  YVRLHTNKGDLNLELHCDLTPKTCENFIRLCKKHYYDGTIFHRSIRNFVIQGGDPTGTGTGGESYWGKPF
  KDEFRPNLSHTGRGILSMANSGPNSNRSQFFITFRSCAYLDKKHTIFGRVVGGFDVLTAMENVESDPKTD
  RPKEEIRIDATTVFVDPYEEADAQIAQERKTQLKVAPETKVKSSQPQAGSQGPQTFRQGVGKYINPAATK
  RAAEEEPSTSATVPMSKKKPSRGFGDFSSW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014337
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014337.4
• RefSeq Size: 4128 bp
• RefSeq ORF: 1563 bp
• Locus ID: 23759
• UniProt ID: Q13356
• Cytogenetics: 22q11.21
• Domains: pro_isomerase, U-box
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]