Cacna1g (NM_001177888) Mouse Tagged ORF Clone

CAT#: MG227527

Cacna1g (tGFP-tagged) - Mouse calcium channel voltage-dependent T type alpha 1G subunit (Cacna1g) transcript variant 3, (10ug)

Product Images

Circular map for MG227527

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cacna1g
• Synonyms: a1G; alpha-1G; Cav3.1d; mKIAA1123; [a]1G
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227527 representing NM_001177888
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGGAAGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGGAGCTTCACGCAGCTCAACG
  ACCTGTCTGGGGCCGGGGGCCGGCAGGGGCCGGGGTCGACGGAAAAGGACCCGGGCAGCGCGGACTCCGA
  GGCGGAGGGGCTGCCCTACCCGGCGCTGGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG
  CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCGTGGTTCGAGCGAGTCAGCATGCTGGTTATTCTCCTCA
  ACTGTGTGACTCTGGGTATGTTCAGGCCGTGTGAGGACATTGCCTGTGACTCCCAACGCTGCCGGATCCT
  GCAGGCCTTCGACGACTTCATCTTTGCCTTCTTTGCTGTGGAAATGGTGGTGAAGATGGTCGCTTTGGGT
  ATCTTTGGGAAGAAATGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTTATCGTCATTGCTGGGA
  TGCTGGAGTACTCGCTGGACCTGCAGAATGTCAGCTTCTCCGCAGTCAGGACAGTCCGTGTGCTGCGACC
  GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATTCTCGTCACATTACTGCTGGATACCTTGCCTATG
  CTGGGCAATGTCCTGCTGCTCTGTTTCTTCGTCTTTTTCATCTTTGGCATCGTTGGTGTTCAGCTGTGGG
  CAGGGCTACTTCGAAACCGATGCTTCCTCCCTGAGAATTTCAGCCTCCCGCTGAGTGTGGACTTGGAGCC
  TTACTACCAGACAGAGAATGAGGACGAGAGCCCCTTCATCTGCTCTCAGCCTCGGGAGAACGGCATGCGA
  TCCTGCAGGAGCGTGCCCACACTGCGCGGGGAAGGCGGTGGTGGTCCACCCTGCGGTCTGGACTATGAGG
  CCTATAACAGTTCCAGCAATACCACCTGTGTCAACTGGAACCAATACTATACCAACTGCTCTGCAGGCGA
  GCACAACCCCTTCAAAGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC
  ATCACACTGGAGGGCTGGGTCGACATCATGTACTTTGTGATGGATGCTCATTCCTTCTACAACTTCATCT
  ACTTCATTCTTCTCATCATCGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATCGCCACACA
  GTTCTCTGAGACCAAGCAACGGGAGAGTCAGCTGATGCGGGAGCAGCGTGTACGATTCCTGTCCAATGCT
  AGCACCCTGGCAAGCTTCTCTGAGCCAGGCAGCTGCTATGAGGAGCTTCTCAAGTACCTGGTGTACATCC
  TCCGCAAAGCAGCCCGCAGGCTGGCCCAGGTCTCTAGGGCTGTAGGCGTGCGGGCTGGGTTGCTCAGCAG
  CCCAGTGGCCCGTGGTGGGCAGGAGCCCCAGCCCAGTGGCAGCTGCTCTCGTTCACACCGTCGTCTGTCT
  GTCCACCACCTGGTCCACCACCATCACCACCACCATCACCACTACCACCTGGGTAATGGGACGCTCAGAG
  TTCCCCGGGCCAGCCCAGAGATCCAGGACAGGGATGCCAATGGGTCCCGCTGGCTCATGCTGCCACCACC
  CTCTACCCCCACTCCCTCTGGGGGCCCTCCGAGGGGTGCGGAGTCTGTACACAGCTTCTACCATGCTGAC
  TGCCACTTGGAGCCAGTCCGTTGCCAGGCGCCCCCTCCCAGGTCCCCATCGGAGGCATCTGGCAGGACTG
  TGGGTAGTGGGAAGGTATACCCCACTGTGCATACCAGCCCTCCACCAGAGATGCTGAAGGATAAGGCACT
  AGTGGAGGTGGCCCCCAGCCCTGGGCCCCCCACCCTCACCAGCTTCAACATCCCACCTGGGCCCTTCAGC
  TCCATGCACAAGCTCCTGGAGACACAGAGTACGGGAGCCTGCCATAGCTCCTGCAAAATCTCCAGCCCTT
  GCTCCAAGGCAGACAGTGGAGCCTGTGGGCCGGACAGTTGTCCCTACTGTGCCCGGACAGGAGCAGGGGA
  GCCAGAGTCCGCTGACCATGAAATGCCTGACTCAGACAGTGAGGCTGTGTATGAGTTCACACAGGACGCT
  CAGCACAGCGACCTCCGGGATCCCCACAGACGGCGACGGCCGAGCCTGGGCCCAGATGCAGAGCCTAGTT
  CTGTGCTGGCCTTCTGGAGGCTGATCTGCGACACATTCCGGAAGATCGTAGATAGCAAATACTTTGGCCG
  GGGAATCATGATCGCTATCCTGGTCAATACCCTCAGCATGGGCATCGAGTACCACGAGCAGCCCGAGGAG
  CTCACCAACGCCCTGGAAATCAGCAACATCGTCTTCACCAGCCTCTTCGCCTTGGAGATGCTGCTGAAGC
  TGCTCGTCTACGGTCCCTTCGGCTACATTAAGAATCCCTACAACATCTTTGATGGCGTCATTGTGGTCAT
  CAGCGTGTGGGAGATTGTGGGCCAGCAGGGAGGTGGCCTGTCGGTGCTGCGGACCTTCCGCCTGATGCGG
  GTGCTGAAGCTAGTTCGCTTCCTGCCGGCACTGCAGCGGCAGCTCGTGGTGCTCATGAAGACCATGGACA
  ACGTGGCCACCTTCTGCATGCTGCTCATGCTCTTCATCTTCATCTTCAGCATCCTGGGCATGCACCTTTT
  TGGTTGCAAGTTCGCATCTGAACGGGATGGGGACACGTTGCCAGACCGGAAGAATTTTGACTCCCTGCTC
  TGGGCCATTGTCACTGTCTTTCAGATTCTGACTCAGGAAGACTGGAATAAAGTCCTTTACAACGGCATGG
  CCTCCACGTCATCTTGGGCTGCTCTTTACTTCATCGCCCTCATGACTTTTGGCAACTACGTGCTCTTTAA
  CCTGCTTGTCGCCATTCTCGTGGAGGGTTTCCAGGCAGAGGGAGATGCCACCAAGTCTGAGTCAGAGCCT
  GATTTCTTTTCGCCCAGTGTGGATGGTGATGGGGACAGGAAGAAGCGCTTGGCCCTGGTGGCCTTGGGAG
  AACACTCGGAACTACGAAAGAGCCTTTTGCCACCTCTCATCATCCACACAGCTGCTACACCGATGTCACT
  GCCCAAGAGCTCCAGCACAGGTGTGGGGGAAGCACTGGGCTCTGGCTCTCGCCGCACCAGTAGCAGTGGG
  TCCGCTGAGCCTGGAACTGCCCATCATGAGATGAAATCACCGCCAAGTGCCCGAAGCTCCCCGCACAGTC
  CCTGGAGCGCAGCAAGCAGCTGGACCAGCAGGCGCTCCAGCCGGAACAGCCTGGGCCGGGCCCCCAGCCT
  GAAGCGTAGGAGCCCAAGCGGGGAGCGGAGGTCCCTGCTGTCTGGAGAGGGTCAGGAGAGCCAGGATGAG
  GAGGAGAGTTCAGAAGAGGACCGGGCCAGCCCAGCAGGCAGTGATCATCGCCACAGGGGTTCCTTGGAAC
  GTGAGGCCAAGAGTTCCTTTGACCTGCCTGACACCCTGCAGGTGCCCGGGCTTCATCGAACAGCCAGCGG
  TCGGAGCTCTGCCTCTGAACACCAAGACTGTAATGGCAAGTCGGCTTCAGGGCGTTTGGCCCGCACCCTG
  CGGGCTGATGACCCCCCACTGGATGGGGATGATGGCGATGATGAGGGCAACCTGAGCAAAGGGGAACGCT
  TACGAGCCTGGGTCCGAGCCCGGCTCCCTGCCTGTTGCCGAGAGCGAGATTCCTGGTCTGCCTATATCTT
  CCCTCCCCAGTCAAGGTTTCGTCTCCTGTGTCACCGGATCATCACCCACAAGATGTTTGACCATGTGGTC
  CTCGTCATCATCTTCCTCAACTGTATCACCATCGCTATGGAGCGCCCCAAAATTGACCCCCACAGCGCTG
  AACGCATCTTCCTGACCCTCTCCAACTACATCTTCACGGCAGTCTTCCTGGCTGAAATGACAGTGAAGGT
  GGTGGCACTGGGCTGGTGCTTTGGGGAGCAGGCCTACCTGCGCAGCAGCTGGAACGTGCTGGACGGCTTG
  CTGGTGCTCATCTCTGTCATCGACATCCTGGTGTCCATGGTCTCTGACAGCGGCACCAAGATTCTCGGCA
  TGCTGAGGGTGCTGCGGCTGCTGCGGACCCTACGTCCACTCAGGGTCATCAGCCGGGCCCAGGGGCTGAA
  GCTGGTGGTAGAGACTCTGATGTCATCCCTCAAACCCATTGGCAACATTGTGGTCATCTGCTGTGCCTTC
  TTCATCATTTTTGGAATTCTTGGGGTGCAGCTCTTCAAAGGGAAGTTCTTCGTGTGTCAGGGTGAGGACA
  CCAGGAACATCACTAACAAGTCCGACTGTGCTGAGGCCAGTTACCGGTGGGTCCGGCACAAGTACAACTT
  TGACAACCTGGGCCAGGCTCTGATGTCCCTGTTTGTGCTGGCCTCCAAGGATGGCTGGGTTGACATCATG
  TATGATGGACTGGATGCTGTGGGAGTGGACCAGCAGCCCATCATGAACCACAACCCTTGGATGCTGCTCT
  ACTTCATCTCCTTCCTCCTCATCGTGGCCTTCTTCGTCCTGAACATGTTTGTGGGCGTGGTGGTGGAGAA
  CTTCCATAAGTGCAGGCAGCACCAGGAGGAGGAGGAGGCGCGGCGGCGGGAGGAGAAGCGACTAAAGAGG
  CTGGAGAAAAAGAGAAGGAATCTAATGTTGGACGATGTAATTGCTTCCGGCAGCTCAGCCAGCGCTGCGT
  CAGAAGCCCAGTGCAAACCCTACTACTCTGACTACTCGCGCTTCCGGCTCCTCGTCCACCACCTGTGTAC
  CAGCCACTACCTGGACCTCTTCATCACTGGTGTCATCGGGCTGAATGTGGTCACGATGGCCATGGAACAT
  TACCAGCAGCCCCAGATCCTGGACGAGGCTCTGAAGATCTGCAACTACATCTTTACCGTCATCTTTGTCT
  TGGAGTCAGTATTCAAACTTGTGGCCTTCGGCTTCCGCCGGTTCTTCCAGGACAGGTGGAACCAGCTGGA
  CCTGGCTATTGTGCTTCTGTCCATCATGGGCATCACGCTGGAAGAGATTGAGGTCAATGCTTCACTGCCC
  ATCAACCCCACCATCATCCGTATCATGAGGGTGCTCCGCATTGCTCGAGTTCTGAAGCTGTTGAAGATGG
  CTGTGGGCATGCGGGCACTGCTGGACACGGTGATGCAGGCCCTGCCCCAGGTGGGGAACCTGGGACTTCT
  CTTCATGCTATTATTTTTCATCTTTGCAGCTCTGGGCGTGGAGCTCTTTGGAGACCTGGAGTGTGATGAG
  ACACACCCTTGTGAGGGCTTGGGCCGGCATGCCACCTTTAGGAACTTTGGTATGGCCTTTCTGACCCTCT
  TCCGAGTCTCCACTGGTGACAACTGGAATGGTATTATGAAGGACACCCTCCGGGACTGTGACCAGGAGTC
  CACCTGCTACAACACCGTCATCTCACCCATCTACTTCGTGTCCTTCGTGCTGACGGCCCAGTTTGTGCTG
  GTCAACGTGGTCATAGCCGTGCTGATGAAGCACCTGGAAGAGAGCAACAAAGAGGCCAAGGAGGAGGCGG
  AGTTGGAGGCGGAGCTGGAGCTAGAGATGAAGACACTCAGCCCGCAGCCCCACTCCCCGCTGGGCAGCCC
  CTTCCTCTGGCCTGGGGTGGAAGGTGTCAATAGCCCTGACAGCCCTAAGCCTGGGGCTCCACACACCACG
  GCCCACATTGGAGCAGCCTCTTCAGGCTTCTCCCTTGAGCACCCCACGATGGTACCTCACACTGAGGAGG
  GGCCAGTCCCCCTAGGACCAGACCTGCTGACTGTGAGGAAGTCTGGTGTCAGCCGGACACACTCTCTGCC
  CAATGACAGCTACATGTGCCGCAATGGGAGCACTGCCGAGAGATCCCTAGGACACAGGGGCTGGGGGCTC
  CCCAAAGCCCAGTCAGGCTCCATCTTGTCTGTTCACTCCCAACCAGCAGACACCAGCTGCATCCTACAGC
  TTCCCAAAGATGCACACTATCTGCTCCAGCCTCATGGGGCTCCCACCTGGGGCGCCATCCCTAAACTACC
  CCCACCTGGCCGCTCCCCTCTGGCTCAGAGGCCTCTCAGGCGCCAGGCAGCAATAAGGACTGACTCCCTG
  GACGTGCAGGGCCTGGGTAGCCGGGAAGACCTGTTGTCAGAGGTGAGTGGGCCCTCCTGCCCTCTGACCC
  GCTCCTCATCCTTCTGGGGCGGGTCGAGCATCCAGGTGCAGCAGCGCTCCGGCAGCCAGAGCAAAGTCTC
  CAAGCACATCCGCCTGCCAGCCCCTTGCCCAGGCCTGGAACCCAGCTGGGCCAAGGACCCTCAAGAGACC
  AGAAGCAGCTTAGAGCTGGACACGGAGCTGAGCTGGATTTCAGGAGACCTCCTGCCCAGCAGTCAGGAAG
  AACCCCTGTCCCCACGGGACTTGAAAAAATGCTACAGTGTAGAGGCCCAGAGCTGCCGGCGCAGGCCTGG
  GTCCTGGCTAGACGAACAGAGGAGACACTCCATCGCTGTCAGCTGCCTGGACAGCGGCTCCCAGCCCCGC
  CTATGTCCAAGCCCCTCAAGCCTCGGGGGCCAACCTCTTGGGGGCCCTGGGAGCCGGCCTAAGAAAAAAC
  TCAGCCCACCCAGTATCTCTATAGACCCCCCGGAGAGCCAGGGCCCTCGGCCCCCATGCAGTCCTGGCGT
  CTGCCTCAGGAGGAGGGCGCCGGCCAGTGACTCGAAGGATCCCTCGGCCTCCAGCCCCCTTGACAGCACG
  GCTGCCTCACCCTCCCCAAAGAAAGATGCGCTGAGTCTCTCTGGTTTGTCTTCTGACCCAACAGACCTGG
  ATCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227527 representing NM_001177888
  Red=Cloning site Green=Tags(s)
  
  MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRP
  RSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALG
  IFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPM
  LGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMR
  SCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQV
  ITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNA
  STLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLS
  VHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHAD
  CHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFS
  SMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDA
  QHSDLRDPHRRRRPSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEE
  LTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMR
  VLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLL
  WAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEP
  DFFSPSVDGDGDRKKRLALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSG
  SAEPGTAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDE
  EESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTL
  RADDPPLDGDDGDDEGNLSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVV
  LVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGL
  LVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAF
  FIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIM
  YDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKR
  LEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEH
  YQQPQILDEALKICNYIFTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLP
  INPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDE
  THPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVL
  VNVVIAVLMKHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTT
  AHIGAASSGFSLEHPTMVPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGL
  PKAQSGSILSVHSQPADTSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSL
  DVQGLGSREDLLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQET
  RSSLELDTELSWISGDLLPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPR
  LCPSPSSLGGQPLGGPGSRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDST
  AASPSPKKDALSLSGLSSDPTDLDP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177888
• ORF Size: 6795 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177888.1, NP_001171359.1
• RefSeq Size: 8148 bp
• RefSeq ORF: 6798 bp
• Locus ID: 12291
• Cytogenetics: 11 D