Sirt1 (NM_019812) Mouse Tagged ORF Clone

CAT#: MG227263

Sirt1 (tGFP-tagged) - Mouse sirtuin 1 (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) (Sirt1) transcript variant 1, (10ug)

Product Images

Circular map for MG227263

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sirt1
• Synonyms: AA673258; S; Si; Sir2; Sir2a; Sir2alpha; SIR2L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227263 representing NM_019812
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGAGGTGGCGCTCGCCCTTCAGGCCGCCGGCTCCCCTTCCGCGGCGGCCGCCATGGAGGCCG
  CGTCGCAGCCGGCGGACGAGCCGCTCCGCAAGAGGCCCCGCCGAGACGGGCCTGGCCTCGGGCGCAGCCC
  GGGCGAGCCGAGCGCAGCAGTGGCGCCGGCGGCCGCGGGGTGTGAGGCGGCGAGCGCCGCGGCCCCGGCG
  GCGCTGTGGCGGGAGGCGGCAGGGGCGGCGGCGAGCGCGGAGCGGGAGGCCCCGGCGACGGCCGTGGCCG
  GGGACGGAGACAATGGGTCCGGCCTGCGGCGGGAGCCGAGGGCGGCTGACGACTTCGACGACGACGAGGG
  CGAGGAGGAGGACGAGGCGGCGGCGGCAGCGGCGGCGGCAGCGATCGGCTACCGAGACAACCTCCTGTTG
  ACCGATGGACTCCTCACTAATGGCTTTCATTCCTGTGAAAGTGATGACGATGACAGAACGTCACACGCCA
  GCTCTAGTGACTGGACTCCGCGGCCGCGGATAGGTCCATATACTTTTGTTCAGCAACATCTCATGATTGG
  CACCGATCCTCGAACAATTCTTAAAGATTTATTACCAGAAACAATTCCTCCACCTGAGCTGGATGATATG
  ACGCTGTGGCAGATTGTTATTAATATCCTTTCAGAACCACCAAAGCGGAAAAAAAGAAAAGATATCAATA
  CAATTGAAGATGCTGTGAAGTTACTGCAGGAGTGTAAAAAGATAATAGTTCTGACTGGAGCTGGGGTTTC
  TGTCTCCTGTGGGATTCCTGACTTCAGATCAAGAGACGGTATCTATGCTCGCCTTGCGGTGGACTTCCCA
  GACCTCCCAGACCCTCAAGCCATGTTTGATATTGAGTATTTTAGAAAAGACCCAAGACCATTCTTCAAGT
  TTGCAAAGGAAATATATCCCGGACAGTTCCAGCCGTCTCTGTGTCACAAATTCATAGCTTTGTCAGATAA
  GGAAGGAAAACTACTTCGAAATTATACTCAAAATATAGATACCTTGGAGCAGGTTGCAGGAATCCAAAGG
  ATCCTTCAGTGTCATGGTTCCTTTGCAACAGCATCTTGCCTGATTTGTAAATACAAAGTTGATTGTGAAG
  CTGTTCGTGGAGACATTTTTAATCAGGTAGTTCCTCGGTGCCCTAGGTGCCCAGCTGATGAGCCACTTGC
  CATCATGAAGCCAGAGATTGTCTTCTTTGGTGAAAACTTACCAGAACAGTTTCATAGAGCCATGAAGTAT
  GACAAAGATGAAGTTGACCTCCTCATTGTTATTGGATCTTCTCTGAAAGTGAGACCAGTAGCACTAATTC
  CAAGTTCTATACCCCATGAAGTGCCTCAAATATTAATAAATAGGGAACCTTTGCCTCATCTACATTTTGA
  TGTAGAGCTCCTTGGAGACTGCGATGTTATAATTAATGAGTTGTGTCATAGGCTAGGTGGTGAATATGCC
  AAACTTTGTTGTAACCCTGTAAAGCTTTCAGAAATTACTGAAAAACCTCCACGCCCACAAAAGGAATTGG
  TTCATTTATCAGAGTTGCCACCAACACCTCTTCATATTTCGGAAGACTCAAGTTCACCTGAAAGAACTGT
  ACCACAAGACTCTTCTGTGATTGCTACACTTGTAGACCAAGCAACAAACAACAATGTTAATGATTTAGAA
  GTATCTGAATCAAGTTGTGTGGAAGAAAAACCACAAGAAGTACAGACTAGTAGGAATGTTGAGAACATTA
  ATGTGGAAAATCCAGATTTTAAGGCTGTTGGTTCCAGTACTGCAGACAAAAATGAAAGAACTTCAGTTGC
  AGAAACAGTGAGAAAATGCTGGCCTAATAGACTTGCAAAGGAGCAGATTAGTAAGCGGCTTGAGGGTAAT
  CAATACCTGTTTGTACCACCAAATCGTTACATATTCCACGGTGCTGAGGTATACTCAGACTCTGAAGATG
  ACGTCTTGTCCTCTAGTTCCTGTGGCAGTAACAGTGACAGTGGCACATGCCAGAGTCCAAGTTTAGAAGA
  ACCCTTGGAAGATGAAAGTGAAATTGAAGAATTCTACAATGGCTTGGAAGATGATACGGAGAGGCCCGAA
  TGTGCTGGAGGATCTGGATTTGGAGCTGATGGAGGGGATCAAGAGGTTGTTAATGAAGCTATAGCTACAA
  GACAGGAATTGACAGATGTAAACTATCCATCAGACAAATCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227263 representing NM_019812
  Red=Cloning site Green=Tags(s)
  
  MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPA
  ALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLL
  TDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDM
  TLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFP
  DLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQR
  ILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKY
  DKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYA
  KLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLE
  VSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGN
  QYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPE
  CAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019812
• ORF Size: 2211 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019812.3
• RefSeq Size: 3920 bp
• RefSeq ORF: 2214 bp
• Locus ID: 93759
• UniProt ID: Q923E4
• Cytogenetics: 10 B4
• Gene Summary: This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]