Vav3 (NM_146139) Mouse Tagged ORF Clone

CAT#: MG226734

Vav3 (tGFP-tagged) - Mouse vav 3 oncogene (Vav3) transcript variant 2, (10ug)

Product Images

Circular map for MG226734

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vav3
• Synonyms: A530094I06Rik; AA986410; Idd18.1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226734 representing NM_146139
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAATTTTTACCTTTGTTTCAGAACAAGGGCCATTCAAACCACCAGAGAAACGGACCAATGGACTTC
  GGAGAGCTTCCAGACAGGTGGACCCAGGTTTGCCGAAGATGCAGGTCATTAGGAACTACACTGGCACCCC
  GGCCCCAGGGCTTCATGAAGGACCACCATTGCATATCCAGGCAGGCGACACTGTTGAACTTCTGAGAGGA
  GATGCACACAGTGTGTTCTGGCAGGGACGAAACTTAGCTTCAGGAGAGGTTGGATTTTTCCCAAGTGATG
  CTGTCAAACCCTCTCCATGTGTACCTAAACCAGTAGATTATTCTTGCCAACCCTGGTATGCTGGCCCAAT
  GGAAAGATTGCAGGCAGAAACTGAACTTATTAACAGGGTGAATAGTACTTACCTTGTAAGGCACAGGACC
  AAAGAATCTGGAGAATATGCAATTAGCATTAAGTACAATAATGAAGCAAAACACATCAAGATTTTAACAA
  GAGATGGCTTTTTTCACATTGCAGAAAATAGAAAATTTAAAAGCTTAATGGAACTTGTGGAGTACTACAA
  GCACCATTCTCTCAAAGAAGGGTTCAGGACCTTAGATACAACGCTACAGTTTCCATACAAGGAGCCAGAA
  CAGCCAGCTGGACAGAGGGGTAATAGAACAGGCAACAGCTTGTTGAGTCCCAAAGTACTGGGTATTGCCA
  TCGCTCGGTACGACTTCTGTGCAAGAGATATGCGGGAGCTCTCCCTATTGAAAGGAGACATGGTGAAGAT
  TTACACGAAGATGAGTGCAAATGGCTGGTGGAGAGGTGAAGTAAATGGCAGGGTGGGCTGGTTTCCATCC
  ACATATGTGGAAGAAGATGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226734 representing NM_146139
  Red=Cloning site Green=Tags(s)
  
  MPIFTFVSEQGPFKPPEKRTNGLRRASRQVDPGLPKMQVIRNYTGTPAPGLHEGPPLHIQAGDTVELLRG
  DAHSVFWQGRNLASGEVGFFPSDAVKPSPCVPKPVDYSCQPWYAGPMERLQAETELINRVNSTYLVRHRT
  KESGEYAISIKYNNEAKHIKILTRDGFFHIAENRKFKSLMELVEYYKHHSLKEGFRTLDTTLQFPYKEPE
  QPAGQRGNRTGNSLLSPKVLGIAIARYDFCARDMRELSLLKGDMVKIYTKMSANGWWRGEVNGRVGWFPS
  TYVEEDE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146139
• ORF Size: 861 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146139.2, NP_666251.1
• RefSeq Size: 3061 bp
• RefSeq ORF: 864 bp
• Locus ID: 57257
• UniProt ID: Q9R0C8
• Cytogenetics: 3 F3
• Gene Summary: Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases (By similarity). Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3-mediated adhesion. Does not affect integrin beta-1-mediated adhesion.[UniProtKB/Swiss-Prot Function]