Diaph2 (NM_172493) Mouse Tagged ORF Clone

CAT#: MG226708

Diap2 (tGFP-tagged) - Mouse diaphanous homolog 2 (Drosophila) (Diap2), (10ug)

Product Images

Circular map for MG226708

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Diaph2
• Synonyms: Dia3; Diap2; Drf2; E430022I22Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226708 representing NM_172493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGCTCGGGGCGGCGGCGTCGGGAGCCGGAGGCGGCGGCGGTGGCGGCGAGGAACACGGCGGGG
  GCCGGAGCAACAAGCGAGGCGCGGGGAACCGGGCCGCCAACGAAGAGGAGACGAGAAACAAACCCAAACT
  GAACATTCAAATAAAAACTTTGGCAGATGATGTGCGTGACCGAATTACAAGTTTCAGAAAATCTGCCACC
  AAGAGAGAAAAGCCTGTTATTCAACATTCTATTGATTATCAAACTGCAGTGGTTGAGATTCCCCCAGCTC
  TGATAGTACATGATGACCGATCTTTGATTTTGTCTGAAAAGGAAGTTCTGGACCTCTTTGAGAAAATGAT
  GGAAGATATGAACCTTAATGAAGAAAAGAAAGCTCCTTTGCGAAAGAAGGATTTTAGCATCAAGCGTGAG
  ATGGTTGTCCAGTATATTTCTGCTACTTCCAAATCTGGTGGCCTGAAAAACAGCAAACATGAGTTCACCC
  TTTCTTCACAAGAATATGTTCATGAATTACGATCTGGCATCTCAGATGAGAAACTTCTCAACTGCCTGGA
  ATCTTTGAGAGTGTCTTTAACCAGCCATCCTGTCAGCTGGGTCAACAACTTTGGCTATGAAGGACTTGGA
  GTCTTACTGGATGTGCTGGAGAAGCTTCTGGACAAAAAACAGCAAGAAAATATCGACAAGAAGAATCAGT
  ACAAAGTCATTCAGTGCCTGAAAGCATTTATGAATAATAAGTTTGGACTACAAAGAATTCTAGGAGACGA
  GAGAAGCCTTTTACTGTTGGCAAGAGCAATTGACCCGAAACAACAGAATATGATGACTGAAATAGTAAAA
  ATCCTTTCTGCTATTTGCATTGTTGGGGAAGAAAACATTCTAGACAAACTTTTAGGGGGCATCACTGCTG
  CAGCTGAACTAAATAATAGAGAACGATTTTCACCAATTGTGGAAGGCTTAGAAAATAATGAAGCCTTGCA
  TCTCCAGGTGGCCTGCATGCAGTTCATAAATGCACTTGTCACCTCTCCATATGATCTTGATTTTCGAATT
  CATTTGAGGAATGAATTCCTTCGTTGTGGATTAAAAGCAATGCTCCCTACTCTTAAAGAGATAGAGAATG
  AAGGGCTTGATATTCAGTTGAGAGTGTTTGAAGAGAACAAAGAAGATGACCTAAGTGAATTATCCCACCG
  TCTCAATGACATTCGAGCTGAAATGGATGATATTAATGAAGTCTACCATCTGCTGTATAATATGCTGAAG
  GACACTGCTGCGGAACCTTACTTATTATCCATTCTCCAACACTTTCTGCTCATCAGAAATGATTACTACA
  TCAGGCCACAGTATTATAAAATCATTGAGGAGTGTGTTTCACAGATAGTGCTACACTGCAGTGGCATGGA
  CCCAGACTTCAAGTACCGGCAGCGCATAGACTTTGATTTCACTCATCTCCTAGATGCTTGTGTAAATAAG
  GCAAAAGTTGAAGAAAATGAGCAAAAAGCAATGGAATTTTCAAAGAAGTTCGATGAAGAATTCACAGCTC
  GTCAGGAAGCTCAAGCAGAGCTTCAGAAAAGAGATGAAAAAATCAAAGAACTGGAAACAGAAATCCAGCA
  GCTTCGAGGACAGGGAGTTCCATCTGCAATTCCAGGTCCACCTCCACCACCTCCACTGCCAGGTGCAGGA
  CCATGCCCACCACCACCACCTCCTCCTCCTCCACCACCTCCACTTCCAGGAGTCGTCCCTCCTCCTCCTC
  CACCATTACCTGGGATGCCTGGTATTCCACCACCACCTCCACCACCTTTGTCTGGAGTACCTCCTCCACC
  ACCACCCCCTGGGGGAGTTTTTCCTCTCCTATCAGGACCAATTGAGCTACCATATGGAATGAAGCAGAAA
  AAATTATATAAGCCTGACATACCTATGAAAAGAATCAACTGGTCCAAGATTGAGCCTAAAGAATTATCCG
  AAAACTGTGTTTGGCTAAAACTTAAAGAAGAGAAGTATGAGAATGCAGATCTATTTGCAAAACTGGCCTT
  GACATTTCCTTCCCAGATGAAAGGTCAAAGGAATACAGAGGCTGCAGAAGAAAATAGGAGTGGGCCTCCA
  AAGAAGAAAGTGAAGGAATTGAGAATTTTGGATACCAAAACAGCCCAGAATCTATCTATCTTCCTTGGAT
  CTTACCGCATGCCATACGAGGAAATAAAGAACATTATTTTGGAGGTTAATGAAGAGATGCTCAGTGAGGC
  CCTAATCCAGAATCTTGTAAAATATCTTCCTGACCAGAACGCACTCCGAGAACTAGCCCAGCTTAAGAGT
  GAATATGATGATCTCTGTGAGCCTGAGCAGTTTGGTGTTGTGATGAGCACGGTGAAAATGTTACGTCCTC
  GTCTCACGAGCATCCTTTTCAAACTCACCTTTGAAGAACATGTCAACAACATCAAACCAAGCATCATAGC
  AGTAACTCTTGCATGTGAAGAACTGAAGAAAAGTGAAAGCTTTAAGAGACTCTTAGAGTTGATTCTTCTG
  GTTGGAAACTACATGAACTCAGGCTCCAGGAATGCCCAGTCTCTAGGTTTTAAGATTAACTTTCTGTGTA
  AGATCAAAGACACAAAATCAGCAGATCAAAAATCCACCCTTTTGCATTTTCTTGCTGAAATCTGTGATGA
  GAAATACCGGGATATCCTGAAATTTCCTGATGAGCTGGAGCATGTGGAAAGCGCAGGCAAAGTTTCAGCT
  CAAATACTCAAGAGCAACCTTGTAGCAATGGAACAAAGCATTCTTCATCTGGAGAAAAACATCAAGAATT
  TCCCACCAGCAGAATCGCACCATGATAAGTTTGTGGAAAAGATGATGAGCTTTACCCAGAATGCCAGAGA
  ACAGTATGACAAACTGTCCACCATGCACAGCAATATGTTGAAGCTCTATGAGAGTCTGGGAGAATACTTC
  ATTTTTGACCCGAACACAGTAAACATGGAAGAATTTTTTGGTGATCTCAACACCTTCAGAACCCTATTTT
  TGGAAGCACTGAAAGAAAACCACAAGAGAAAGGAAATGGAAGAGAAGAGCAGGAGAGCAAAGCTTGCAAA
  AGAGAAAGCGGAACAAGAGAAGCTGGAACGCCAGAAGAAGAAGAAGCAACTCATTGACATAAACAAAGAG
  GGTGATGAGACGGGAGTGATGGATAATCTTCTGGAAGCCCTACAGTCAGGTGCAGCATTCAGAGACCGCA
  GAAAGCGAATTCCAAGAAATCCAGATAACAGACGGCCCCCTCTGGAAAGATCTCGCTCTCGCCACAATGG
  AGCCATGTCATCCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226708 representing NM_172493
  Red=Cloning site Green=Tags(s)
  
  MEELGAAASGAGGGGGGGEEHGGGRSNKRGAGNRAANEEETRNKPKLNIQIKTLADDVRDRITSFRKSAT
  KREKPVIQHSIDYQTAVVEIPPALIVHDDRSLILSEKEVLDLFEKMMEDMNLNEEKKAPLRKKDFSIKRE
  MVVQYISATSKSGGLKNSKHEFTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSHPVSWVNNFGYEGLG
  VLLDVLEKLLDKKQQENIDKKNQYKVIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVK
  ILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRI
  HLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLK
  DTAAEPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNK
  AKVEENEQKAMEFSKKFDEEFTARQEAQAELQKRDEKIKELETEIQQLRGQGVPSAIPGPPPPPPLPGAG
  PCPPPPPPPPPPPPLPGVVPPPPPPLPGMPGIPPPPPPPLSGVPPPPPPPGGVFPLLSGPIELPYGMKQK
  KLYKPDIPMKRINWSKIEPKELSENCVWLKLKEEKYENADLFAKLALTFPSQMKGQRNTEAAEENRSGPP
  KKKVKELRILDTKTAQNLSIFLGSYRMPYEEIKNIILEVNEEMLSEALIQNLVKYLPDQNALRELAQLKS
  EYDDLCEPEQFGVVMSTVKMLRPRLTSILFKLTFEEHVNNIKPSIIAVTLACEELKKSESFKRLLELILL
  VGNYMNSGSRNAQSLGFKINFLCKIKDTKSADQKSTLLHFLAEICDEKYRDILKFPDELEHVESAGKVSA
  QILKSNLVAMEQSILHLEKNIKNFPPAESHHDKFVEKMMSFTQNAREQYDKLSTMHSNMLKLYESLGEYF
  IFDPNTVNMEEFFGDLNTFRTLFLEALKENHKRKEMEEKSRRAKLAKEKAEQEKLERQKKKKQLIDINKE
  GDETGVMDNLLEALQSGAAFRDRRKRIPRNPDNRRPPLERSRSRHNGAMSSK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172493
• ORF Size: 3306 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172493.2, NP_766081.1
• RefSeq Size: 8456 bp
• RefSeq ORF: 3309 bp
• Locus ID: 54004
• Cytogenetics: X E3
• Gene Summary: May be involved in oogenesis.[UniProtKB/Swiss-Prot Function]