Carf (NM_139150) Mouse Tagged ORF Clone

CAT#: MG226553

Carf (tGFP-tagged) - Mouse calcium response factor (Carf), (10ug)

Product Images

Circular map for MG226553

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Carf
• Synonyms: Als2cr8; ECBRC-FC1; ECBRC-FC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226553 representing NM_139150
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGCGTCATTCCTTAGCGGGCAACTGTGACGATGGTGAGAAGTCAGAAAGAGAGGCTCAGGGAT
  TCGAGCATCGGACCTGTATGGACTCTGGGGATCCTTCCTTTGGGCAAAATGATCCCCCTACCATTTTGCC
  CATTACTGCTCCTAAAACATATGATTCACTCACATCACAGGATATGCCAGAGACACTGACTGACACACAG
  GCCCTTCATGAAGAGCAATTTCATCTTTTGGACCAAAATGGGCAGCCTATTCAATATGATCTTCAGTCTT
  TGGGAAATTCTACTGCACAAATGACAGGGATGGAGCAAGTGGCCATACCTCAGGAGCCCTTTGCAGATGA
  ACATAGCCCTCAAGATATCTCTGAAGAGAAACCCACTGGTGTAAGAGCAGATGTTCTCGAAGACAGCACC
  AGCAATTACGGCCTTCGCTCACCAGCATCCCTCGTGCTACCCAAAAAGGCAGGAGCCAGACTTGTTGAAG
  AACCTTTGCTGGCTCCTCTCCAGCCACTTTCTTGTAACACACCCATGTGGGCCTGCCGTCTTAGGAGTTG
  TGAGAAAATTGGAGATTCCTACCGTGGCTACTGTGTAAGTGAGACTGAATTAGAAAGTGTCCTAACTTTT
  CACAAGCAGCAAACACAGACTGTCTGGGGTACTCGCCAGTCTCCAAGCCCAGCCAAACCTGCCACACGCT
  TGATGTGGAAGTCTCAGTATGTCCCATATGACGGAATCCCATTTGTCAATGCAGGAAGTCGAGCTGTGGT
  AATGGAGTGTCAGTATGGACCAAGGAGAAAAGGTTTTCAGTTAAAAAAAATCAGTGAGCAAGAAAGTAGG
  TCTTGTCATCTCTATAAAGCTACTTGTCCAGCCAGGATATACATTAAAAAGGTACAGAAATTTCCTGAAT
  ATAGAGTACCTACAGACCCCCAAATTGACAGGAAGATAATCAGACTGGAGCAGGAGAAAGCCTTCACTAT
  GCTGAAGAAGAACCTGATGGATGCTGGTGGTGTACTTCGATGGTATGTGCAGTTACCTACACAGCAAGCT
  CATCAGTATCATGAATCAGAGACTCCTGGTGTCCCTTTGTCACCGTCACCTTTTCCTATGTCTCCTCTTG
  AAGAAGAGGAAGCTATAGTCAGAGATGAGAACTGTGCGTTGCCCTCTCGTCTCCACCCGCAAGTAGCGCA
  TAAGATTCAGGAACTAGTGTCACAGGGAGTAGGACAAGTGTATGCAGTAAGGAAGCAGCTAAGAAAATTT
  GTGGAAAGAGAACTGTTCAAACCTGATGAGATACCTGAAAGACATAATTTATCTTATTTTCCAACTGTAA
  ATGATATAAAAAATCATATCCATGAGGTACAGAAATCCTTGAGGACTGGAGATGTGGTATATAACTCAGA
  GATTATTCCAGCAACGCTTCAGTGGACGACAGATAGTGGGAACATCCTCAGAGAGACTGTAACAGTTACA
  TTTGCAGAAGGAAATTTACTAGGAGAACCGATTCCCAGCAAAATGGGAACAAGTCAGACACAGACTGCTG
  TGTCTCCAGAGCCACTTTCTTCATTTCCACCCAAAATATTTACACACTTTCAGGCTTTGAAGTTACAGCC
  AAGACTCTCCTCTCCTGATGGCTCACAAGCTTTGGTATCAGTAGATAGCCATGCATCCTCTAGTCCTCCT
  GGCCTTGTGGATACTGTAGGAAATGCTGAAGTGGATAACCACTCTGTACTGCTTGGTCAGAGTCAAAACC
  CTGGGACAGATACATGCCTAACCCAAGACAACAGCACCTCCTCCTCCACGGGCCACCTCCCAGAATCAGT
  TCCGAATCCAGTTGCAGAGGATCAGCTGCTGGAAGGTGAAGATGTTGAGGATGCAGGGAATCCGGAAGGA
  AGTGTAAATAGGACTCTGTTGGGAGATGTACAAACCGTTCCAATACAGATCATAGACAGCCGTCCAGTGC
  TTGTTGAAGAAAGTCTGTCTAAGAACCAAGTTAAACAAGAAACCAATGAGCCAACACTGTCTACAGAAGC
  AAAAACTTTCCTGGACTGTAAAAAAATATCTGCTACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226553 representing NM_139150
  Red=Cloning site Green=Tags(s)
  
  MEQRHSLAGNCDDGEKSEREAQGFEHRTCMDSGDPSFGQNDPPTILPITAPKTYDSLTSQDMPETLTDTQ
  ALHEEQFHLLDQNGQPIQYDLQSLGNSTAQMTGMEQVAIPQEPFADEHSPQDISEEKPTGVRADVLEDST
  SNYGLRSPASLVLPKKAGARLVEEPLLAPLQPLSCNTPMWACRLRSCEKIGDSYRGYCVSETELESVLTF
  HKQQTQTVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSRAVVMECQYGPRRKGFQLKKISEQESR
  SCHLYKATCPARIYIKKVQKFPEYRVPTDPQIDRKIIRLEQEKAFTMLKKNLMDAGGVLRWYVQLPTQQA
  HQYHESETPGVPLSPSPFPMSPLEEEEAIVRDENCALPSRLHPQVAHKIQELVSQGVGQVYAVRKQLRKF
  VERELFKPDEIPERHNLSYFPTVNDIKNHIHEVQKSLRTGDVVYNSEIIPATLQWTTDSGNILRETVTVT
  FAEGNLLGEPIPSKMGTSQTQTAVSPEPLSSFPPKIFTHFQALKLQPRLSSPDGSQALVSVDSHASSSPP
  GLVDTVGNAEVDNHSVLLGQSQNPGTDTCLTQDNSTSSSTGHLPESVPNPVAEDQLLEGEDVEDAGNPEG
  SVNRTLLGDVQTVPIQIIDSRPVLVEESLSKNQVKQETNEPTLSTEAKTFLDCKKISAT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139150
• ORF Size: 2067 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139150.5, NP_631889.1
• RefSeq Size: 2604 bp
• RefSeq ORF: 2070 bp
• Locus ID: 241066
• UniProt ID: Q8VHI4
• Cytogenetics: 1 C2
• Gene Summary: Acts as a transcriptional activator that mediates the calcium- and neuron-selective induction of BDNF exon III transcription. Binds to the consensus calcium-response element CaRE1 5'-CTATTTCGAG-3' sequence (By similarity).[UniProtKB/Swiss-Prot Function]