Klf2 (NM_008452) Mouse Tagged ORF Clone

CAT#: MG226357

Klf2 (tGFP-tagged) - Mouse Kruppel-like factor 2 (lung) (Klf2), (10ug)

Product Images

Circular map for MG226357

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Klf2
• Synonyms: Lklf
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226357 representing NM_008452
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCAGCGAGCCTATCTTGCCGTCCTTTGCCACTTTCGCCAGCCCGTGCGAGCGCGGCCTCCAGG
  AGCGCTGGCCGCGAAATGAACCCGAGGCGGGCGGCACGGATGAGGACCTAAACAACGTGTTGGACTTCAT
  CCTCTCCATGGGATTGGACGGTCTGGGCGCCGAGAATCCTCCCGAGCCCCCGCCGCAGCCCCCGCCGCCT
  GCCTTCTACTACCCGGAGCCGGGTGCTCCGCCGCCCTACAGCATCCCCGCGGCCAGCCTGGGAACAGAGC
  TGCTGCGCCCCGACCTGGACCCGCCTCAGGGGCCGGCTCTGCACGGCCGCTTCCTCCTTGCGCCTCCCGG
  GCGGCTAGTGAAGGCCGAGCCCCCCGAGGTGGACGGCGGCGGCTACGGCTGCGCTCCGGGCCTGGCCCAC
  GGACCGCGCGGGCTGAAGCTCGAGGGCGCCCCAGGAGCGACAGGTGCATGCATGCGGGGTCCCGCCGGCC
  GCCCCCCGCCGCCCCCGGACACGCCGCCGCTCAGCCCCGACGGCCCCCTGCGCATCCCGGCGTCCGGTCC
  ACGCAACCCGTTCCCGCCGCCCTTCGGTCCCGGCCCCAGCTTCGGCGGCCCCGGCCCCGCGTTGCACTAC
  GGGCCTCCCGCGCCTGGCGCCTTCGGTCTTTTCGAGGACGCGGCGGCAGCGGCGGCGGCGCTGGGCTTGG
  CTCCACCTGCCACGCGCGGTCTCCTCACGCCGCCCTCGTCCCCGCTGGAGCTGCTGGAGGCCAAGCCCAA
  ACGCGGCCGCCGCTCCTGGCCCCGCAAGCGCGCCGCCACACATACTTGCAGCTACACCAACTGCGGCAAG
  ACCTACACCAAGAGCTCGCACCTAAAGGCGCATCTGCGTACACACACAGGTGAGAAGCCTTATCATTGCA
  ACTGGGAAGGATGCGGCTGGAAGTTCGCGCGCTCTGACGAGCTTACCCGCCACTACCGAAAGCACACAGG
  TCACAGACCCTTTCAGTGCCACTTGTGCGACCGGGCCTTCTCCAGGTCCGACCACCTGGCCTTGCACATG
  AAGCGACACATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226357 representing NM_008452
  Red=Cloning site Green=Tags(s)
  
  MALSEPILPSFATFASPCERGLQERWPRNEPEAGGTDEDLNNVLDFILSMGLDGLGAENPPEPPPQPPPP
  AFYYPEPGAPPPYSIPAASLGTELLRPDLDPPQGPALHGRFLLAPPGRLVKAEPPEVDGGGYGCAPGLAH
  GPRGLKLEGAPGATGACMRGPAGRPPPPPDTPPLSPDGPLRIPASGPRNPFPPPFGPGPSFGGPGPALHY
  GPPAPGAFGLFEDAAAAAAALGLAPPATRGLLTPPSSPLELLEAKPKRGRRSWPRKRAATHTCSYTNCGK
  TYTKSSHLKAHLRTHTGEKPYHCNWEGCGWKFARSDELTRHYRKHTGHRPFQCHLCDRAFSRSDHLALHM
  KRHM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008452
• ORF Size: 1062 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008452.2, NP_032478.2
• RefSeq Size: 1816 bp
• RefSeq ORF: 1065 bp
• Locus ID: 16598
• UniProt ID: Q60843
• Cytogenetics: 8 B3.3
• Gene Summary: Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription. Might be involved in transcriptional regulation by modulating the binding of the RARA nuclear receptor to RARE DNA elements (By similarity).[UniProtKB/Swiss-Prot Function]