Ntrk2 (NM_001025074) Mouse Tagged ORF Clone

CAT#: MG226130

Ntrk2 (tGFP-tagged) - Mouse neurotrophic tyrosine kinase receptor type 2 (Ntrk2) transcript variant 1, (10ug)

Product Images

Circular map for MG226130

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ntrk2
• Synonyms: GP145-TrkB/GP95-TrkB; Tkrb; trk-B; trkB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226130 representing NM_001025074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCCTGGCTGAAGTGGCATGGACCCGCCATGGCGCGGCTCTGGGGCTTATGCCTGCTGGTCTTGG
  GCTTCTGGAGGGCCTCTCTCGCCTGCCCGACGTCCTGCAAATGCAGTTCCGCTAGGATTTGGTGTACTGA
  GCCTTCTCCAGGCATCGTGGCATTCCCGAGGTTGGAACCTAACAGCGTTGACCCGGAGAACATCACGGAA
  ATTCTCATTGCAAACCAGAAAAGGCTAGAAATCATCAATGAAGATGACGTTGAAGCTTACGTGGGGCTGA
  GAAACCTTACAATTGTGGATTCCGGCTTAAAGTTTGTGGCTTACAAAGCGTTTCTGAAAAACAGCAACCT
  GCGGCACATAAATTTCACACGAAACAAGCTGACGAGTTTGTCCAGGAGACATTTCCGCCACCTTGACTTG
  TCTGACCTGATCCTGACGGGTAATCCGTTCACGTGCTCCTGCGACATCATGTGGCTCAAGACTCTCCAGG
  AGACTAAATCCAGCCCCGACACTCAGGATTTGTACTGCCTCAATGAGAGCAGCAAGAACATGCCCCTGGC
  GAACCTGCAGATACCCAATTGTGGTCTGCCATCTGCACGTCTGGCTGCTCCTAACCTCACCGTGGAGGAA
  GGAAAGTCTGTGACCCTTTCCTGCAGTGTGGGGGGTGACCCACTCCCCACCTTGTACTGGGACGTTGGGA
  ATTTGGTTTCCAAGCACATGAATGAAACAAGCCACACACAGGGCTCCTTAAGGATAACGAACATTTCATC
  TGATGACAGTGGAAAGCAAATCTCTTGTGTGGCAGAAAACCTTGTAGGAGAAGATCAAGATTCTGTGAAC
  CTCACTGTGCATTTTGCGCCAACTATCACGTTTCTCGAGTCTCCAACCTCAGATCACCACTGGTGCATTC
  CATTCACTGTGAGAGGCAACCCCAAGCCTGCGCTTCAGTGGTTCTACAATGGGGCCATACTGAATGAGTC
  CAAGTACATCTGTACTAAGATCCACGTCACCAATCACACGGAGTACCATGGCTGCCTCCAGCTGGATAAC
  CCCACTCATATGAATAACGGAGACTACACCCTGATGGCCAAGAACGAGTATGGGAAGGATGAGAGACAGA
  TCTCCGCTCACTTCATGGGCCGGCCTGGAGTCGACTACGAGACAAACCCAAATTACCCTGAAGTCCTCTA
  TGAAGACTGGACCACGCCAACTGACATTGGGGATACTACGAACAAAAGTAATGAAATCCCCTCCACGGAT
  GTTGCTGACCAAAGCAATCGGGAGCATCTCTCGGTCTATGCCGTGGTGGTGATTGCATCTGTGGTGGGAT
  TCTGCCTGCTGGTGATGTTGCTCCTGCTCAAGTTGGCGAGACATTCCAAGTTTGGCATGAAAGGCCCAGC
  TTCGGTCATCAGCAACGACGATGACTCTGCCAGCCCCCTCCACCACATCTCCAATGGGAGTAACACTCCA
  TCTTCTTCGGAGGGCGGTCCCGACGCTGTCATTATTGGAATGACCAAGATTCCTGTTATTGAAAACCCCC
  AGTACTTTGGCATCACCAACAGTCAGCTCAAGCCAGACACATTTGTTCAGCATATCAAGAGACACAACAT
  CGTTCTGAAGAGGGAACTTGGGGAAGGAGCCTTCGGGAAAGTTTTCCTTGCCGAGTGCTACAACCTCTGC
  CCAGAGCAGGATAAGATCCTGGTGGCTGTGAAGACGCTGAAGGACGCCAGCGACAATGCACGCAAGGACT
  TTCATCGGGAAGCTGAGCTGCTGACCAACCTCCAGCACGAGCACATTGTCAAGTTCTACGGTGTCTGTGT
  GGAGGGCGACCCACTCATCATGGTCTTTGAGTACATGAAGCACGGGGACCTCAACAAGTTCCTTAGGGCA
  CACGGGCCCGACGCAGTGCTGATGGCAGAGGGTAACCCGCCCACAGAGCTGACGCAGTCGCAGATGCTGC
  ACATCGCTCAGCAAATCGCAGCAGGTATGGTCTACCTGGCGTCCCAACACTTTGTGCACCGTGACCTGGC
  CACCCGGAACTGCCTGGTGGGAGAGAACCTGCTGGTGAAAATTGGGGACTTTGGGATGTCCCGAGATGTG
  TACAGCACCGACTACTATCGGGTCGGTGGCCACACAATGTTGCCCATCCGATGGATGCCTCCAGAGAGCA
  TCATGTACAGGAAATTCACCACCGAGAGCGACGTCTGGAGCCTGGGCGTTGTGTTGTGGGAGATCTTCAC
  CTACGGCAAGCAGCCCTGGTATCAGCTATCGAACAATGAGGTGATAGAGTGCATCACCCAGGGAAGAGTC
  CTTCAGCGGCCTCGAACGTGTCCCCAGGAGGTGTATGAGCTCATGCTTGGATGCTGGCAGCGGGAACCAC
  ACACCCGGAAGAACATCAAGAGCATCCACACCCTCCTTCAGAACTTGGCCAAGGCATCTCCCGTCTACCT
  GGATATCCTAGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226130 representing NM_001025074
  Red=Cloning site Green=Tags(s)
  
  MSPWLKWHGPAMARLWGLCLLVLGFWRASLACPTSCKCSSARIWCTEPSPGIVAFPRLEPNSVDPENITE
  ILIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAYKAFLKNSNLRHINFTRNKLTSLSRRHFRHLDL
  SDLILTGNPFTCSCDIMWLKTLQETKSSPDTQDLYCLNESSKNMPLANLQIPNCGLPSARLAAPNLTVEE
  GKSVTLSCSVGGDPLPTLYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVN
  LTVHFAPTITFLESPTSDHHWCIPFTVRGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDN
  PTHMNNGDYTLMAKNEYGKDERQISAHFMGRPGVDYETNPNYPEVLYEDWTTPTDIGDTTNKSNEIPSTD
  VADQSNREHLSVYAVVVIASVVGFCLLVMLLLLKLARHSKFGMKGPASVISNDDDSASPLHHISNGSNTP
  SSSEGGPDAVIIGMTKIPVIENPQYFGITNSQLKPDTFVQHIKRHNIVLKRELGEGAFGKVFLAECYNLC
  PEQDKILVAVKTLKDASDNARKDFHREAELLTNLQHEHIVKFYGVCVEGDPLIMVFEYMKHGDLNKFLRA
  HGPDAVLMAEGNPPTELTQSQMLHIAQQIAAGMVYLASQHFVHRDLATRNCLVGENLLVKIGDFGMSRDV
  YSTDYYRVGGHTMLPIRWMPPESIMYRKFTTESDVWSLGVVLWEIFTYGKQPWYQLSNNEVIECITQGRV
  LQRPRTCPQEVYELMLGCWQREPHTRKNIKSIHTLLQNLAKASPVYLDILG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025074
• ORF Size: 2463 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025074.2, NP_001020245.1
• RefSeq Size: 4614 bp
• RefSeq ORF: 2466 bp
• Locus ID: 18212
• UniProt ID: P15209
• Cytogenetics: 13 31.2 cM
• Gene Summary: Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.[UniProtKB/Swiss-Prot Function]