Rtn4 (NM_194054) Mouse Tagged ORF Clone

CAT#: MG226124

Rtn4 (tGFP-tagged) - Mouse reticulon 4 (Rtn4) transcript variant 1, (10ug)

Product Images

Circular map for MG226124

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rtn4
• Synonyms: 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; mKIAA0886; mKIAA4153; NgA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226124 representing NM_194054
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACATAGACCAGTCGTCGCTGGTCTCCTCGTCCGCGGATAGCCCGCCCCGGCCCCCGCCCGCTT
  TCAAGTACCAGTTCGTGACGGAGCCCGAGGACGAGGAGGACGAGGAAGACGAGGAGGAGGAGGAGGACGA
  CGAGGACCTGGAGGAATTGGAGGTGCTGGAGAGGAAGCCCGCAGCCGGGCTGTCCGCGGCTCCGGTGCCC
  CCCGCCGCCGCACCGCTGCTGGACTTCAGCAGCGACTCGGTGCCCCCCGCGCCCCGCGGGCCGCTGCCGG
  CCGCGCCCCCCACCGCCCCTGAGAGGCAGCCGTCCTGGGAACGCAGCCCCGCGGCGTCCGCGCCATCCCT
  GCCGCCCGCTGCCGCAGTCCTGCCCTCCAAGCTCCCGGAGGACGACGAGCCTCCAGCGCGGCCTCCGGCG
  CCAGCCGGCGCGAGCCCCCTAGCGGAGCCCGCCGCGCCCCCTTCCACGCCGGCCGCGCCCAAGCGCAGGG
  GCTCGGGCTCAGTGGATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACCCTCCTCTGC
  AGAAAAAATTATGGATTTGAAGGAGCAGCCAGGTAACACTGTTTCGTCTGGTCAAGAGGATTTCCCATCT
  GTCCTGTTTGAAACTGCTGCCTCTCTTCCTTCTCTATCTCCTCTCTCAACTGTTTCTTTTAAAGAACACG
  GATACCTTGGTAACTTATCAGCAGTGGCATCCACAGAAGGAACTATTGAAGAAACTTTAAATGAAGCTTC
  TAGAGAATTGCCAGAGAGGGCAACAAATCCATTTGTAAATAGAGAGTCAGCAGAGTTTTCAGTATTAGAA
  TACTCAGAAATGGGATCATCTTTCAATGGCTCCCCAAAAGGAGAGTCAGCCATGTTAGTAGAAAACACTA
  AGGAAGAAGTAATTGTGAGGAGTAAAGACAAAGAGGATTTAGTTTGTAGTGCAGCCCTTCATAATCCACA
  AGAGTCACCTGCGACCCTTACTAAAGTGGTTAAAGAAGACGGAGTTATGTCTCCAGAAAAGACAATGGAC
  ATTTTTAATGAAATGAAAATGTCAGTGGTAGCACCTGTGAGGGAAGAGTATGCAGATTTTAAGCCATTTG
  AACAAGCATGGGAAGTGAAAGATACTTATGAGGGAAGTAGGGATGTGCTGGCTGCTAGAGCTAATATGGA
  AAGTAAAGTGGACAAAAAATGCTTTGAAGATAGCCTGGAGCAAAAAGGTCATGGGAAGGATAGTGAAAGC
  AGAAATGAGAATGCTTCTTTCCCCAGGACCCCAGAACTTGTGAAGGACGGCTCCAGAGCGTACATCACCT
  GTGATTCCTTTAGCTCAGCAACCGAGAGTACTGCAGCAAACATTTTCCCTGTGCTAGAAGATCACACTTC
  AGAAAACAAAACAGATGAAAAAAAAATAGAAGAAAGGAAGGCCCAAATTATAACAGAGAAGACTAGCCCC
  AAAACGTCAAATCCTTTCCTTGTAGCAATACATGATTCTGAGGCAGATTATGTCACAACAGATAATTTAT
  CAAAGGTGACTGAGGCAGTAGTGGCAACCATGCCTGAAGGTCTAACGCCAGATTTAGTTCAGGAAGCATG
  TGAAAGTGAACTGAACGAAGCCACAGGTACAAAGATTGCTTATGAAACAAAAGTGGACTTGGTCCAGACA
  TCAGAAGCTATACAAGAGTCAATTTACCCCACAGCACAGCTTTGCCCATCATTTGAGGAAGCTGAAGCAA
  CTCCGTCACCAGTTTTGCCTGATATTGTTATGGAAGCGCCATTAAATTCTCTCCTTCCAAGCACTGGTGC
  TTCTGTAGCGCAGCCCAGTGCATCCCCACTAGAAGTACCGTCTCCAGTTAGTTATGACGGTATAAAGCTT
  GAGCCTGAAAATCCCCCACCATATGAAGAAGCCATGAGTGTAGCACTAAAAACATCGGACTCAAAGGAAG
  AAATTAAAGAGCCTGAAAGTTTTAATGCAGCTGCTCAGGAAGCAGAAGCTCCTTATATATCCATTGCATG
  TGATTTAATTAAAGAAACAAAGCTCTCCACTGAGCCAAGTCCAGAGTTCTCTAATTATTCAGAAATAGCA
  AAATTTGAGAAGTCGGTGCCTGATCACTGTGAGCTCGTGGATGATTCCTCACCCGAATCTGAACCAGTTG
  ACTTATTTAGTGATGATTCAATTCCTGAAGTCCCACAAACACAAGAGGAGGCTGTGATGCTAATGAAGGA
  GAGTCTCACTGAAGTGTCTGAGACAGTAACACAACACAAACATAAGGAGAGACTTAGTGCTTCACCTCAG
  GAGGTAGGAAAGCCATATTTAGAGTCTTTTCAGCCCAATTTACATATTACAAAAGATGCTGCATCTAATG
  AAATTCCAACATTGACCAAAAAGGAGACAATTTCTTTGCAAATGGAAGAGTTTAATACTGCAATTTATTC
  CAATGATGACTTACTTTCTTCTAAGGAAGACAAAATGAAAGAAAGTGAAACATTTTCCGATTCATCTCCC
  ATTGAGATAATAGATGAGTTTCCCACATTTGTCAGTGCTAAAGATGATTCTCCTAAGGAGTACACTGACC
  TAGAAGTATCCAACAAAAGTGAAATTGCTAATGTCCAGAGCGGGGCCAATTCGTTGCCTTGCTCAGAATT
  GCCCTGTGACCTTTCTTTCAAGAATACATATCCTAAAGATGAAGCACATGTCTCAGATGAATTCTCCAAA
  AGTAGGTCCAGTGTATCTAAGGTGCCCTTATTGCTTCCAAATGTTTCTGCTTTGGAATCTCAAATAGAAA
  TGGGCAACATAGTTAAACCCAAAGTACTTACGAAAGAAGCAGAGGAAAAACTTCCTTCTGATACAGAGAA
  AGAGGACAGATCCCTGACAGCTGTATTGTCAGCAGAGCTGAATAAAACTTCAGTTGTTGACCTCCTGTAC
  TGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCTTATTCCTGCTGCTGTCTCTGACAGTGT
  TCAGCATTGTCAGTGTAACGGCCTACATTGCCTTGGCCCTGCTCTCTGTGACTATCAGCTTTAGGATATA
  TAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATTTGGAATCTGAA
  GTTGCCATATCAGAGGAATTGGTTCAGAAATATAGTAATTCTGCTCTTGGTCATGTGAACAGCACAATAA
  AAGAATTGAGGCGTCTCTTCTTAGTTGATGATTTAGTTGATTCCCTGAAGTTTGCAGTGTTGATGTGGGT
  ATTTACTTACGTTGGTGCCTTGTTCAATGGTTTGACACTACTGATTTTAGCTCTGATCTCACTCTTCAGT
  ATTCCTGTTATATATGAACGGCATCAGGCGCAGATAGATCATTATCTAGGACTTGCAAACAAGAGCGTTA
  AGGATGCCATGGCCAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226124 representing NM_194054
  Red=Cloning site Green=Tags(s)
  
  MEDIDQSSLVSSSADSPPRPPPAFKYQFVTEPEDEEDEEDEEEEEDDEDLEELEVLERKPAAGLSAAPVP
  PAAAPLLDFSSDSVPPAPRGPLPAAPPTAPERQPSWERSPAASAPSLPPAAAVLPSKLPEDDEPPARPPA
  PAGASPLAEPAAPPSTPAAPKRRGSGSVDETLFALPAASEPVIPSSAEKIMDLKEQPGNTVSSGQEDFPS
  VLFETAASLPSLSPLSTVSFKEHGYLGNLSAVASTEGTIEETLNEASRELPERATNPFVNRESAEFSVLE
  YSEMGSSFNGSPKGESAMLVENTKEEVIVRSKDKEDLVCSAALHNPQESPATLTKVVKEDGVMSPEKTMD
  IFNEMKMSVVAPVREEYADFKPFEQAWEVKDTYEGSRDVLAARANMESKVDKKCFEDSLEQKGHGKDSES
  RNENASFPRTPELVKDGSRAYITCDSFSSATESTAANIFPVLEDHTSENKTDEKKIEERKAQIITEKTSP
  KTSNPFLVAIHDSEADYVTTDNLSKVTEAVVATMPEGLTPDLVQEACESELNEATGTKIAYETKVDLVQT
  SEAIQESIYPTAQLCPSFEEAEATPSPVLPDIVMEAPLNSLLPSTGASVAQPSASPLEVPSPVSYDGIKL
  EPENPPPYEEAMSVALKTSDSKEEIKEPESFNAAAQEAEAPYISIACDLIKETKLSTEPSPEFSNYSEIA
  KFEKSVPDHCELVDDSSPESEPVDLFSDDSIPEVPQTQEEAVMLMKESLTEVSETVTQHKHKERLSASPQ
  EVGKPYLESFQPNLHITKDAASNEIPTLTKKETISLQMEEFNTAIYSNDDLLSSKEDKMKESETFSDSSP
  IEIIDEFPTFVSAKDDSPKEYTDLEVSNKSEIANVQSGANSLPCSELPCDLSFKNTYPKDEAHVSDEFSK
  SRSSVSKVPLLLPNVSALESQIEMGNIVKPKVLTKEAEEKLPSDTEKEDRSLTAVLSAELNKTSVVDLLY
  WRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESE
  VAISEELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFS
  IPVIYERHQAQIDHYLGLANKSVKDAMAKIQAKIPGLKRKAE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194054
• ORF Size: 3486 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194054.3, NP_918943.1
• RefSeq Size: 6165 bp
• RefSeq ORF: 3489 bp
• Locus ID: 68585
• UniProt ID: Q99P72
• Cytogenetics: 11 A3.3
• Gene Summary: Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities.[UniProtKB/Swiss-Prot Function]