Tdrd1 (NM_031387) Mouse Tagged ORF Clone

CAT#: MG226058

Tdrd1 (tGFP-tagged) - Mouse tudor domain containing 1 (Tdrd1) transcript variant 4, (10ug)

Product Images

Circular map for MG226058

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tdrd1
• Synonyms: MTR-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226058 representing NM_031387
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCCACGGAATAATTTGGAAGCCTCTACTTGTAAAATGGCAGAGCCATTTAATTTTGAGAAGAAGG
  AAAGCAAGCCCCCGCCCCAGGACCCTCTCAGAAGTCCTGTAGCACAGCATAACCACCCCACCTTCAGGCT
  GAAAAGCCCAGAGAACGGGAATACAAAGAACAACTTTTTGCTTTGTGAACAAAACAAACAGTATTTAGCT
  AGTCAGGAGGACAGTTCGGTGGTCTCTTCAAACCCTGCTGTTGTCAATGGAGAAGTCGGTGGGTCCAAAG
  GAGACAGGAAGCCGCCACCAACAGGAAACCCAGTGTCACCATTAAGTCTTGGAAACAGTTCACCTCCTAA
  CCAAGTAAAAACTAAGCCCAGCAGTAATGTGACTCCTGAAAAATCAAAAAAATCTCACAAGTTGTTTGAG
  AATGCCTTGTCCGTAAATAATCCAGCACTCTTCAACTCGTTAGGACCGCCCCTTCGGTCAACAACCTGCC
  ACCGCTGTGGCCTGTTTGGGTCGCTGAGGTGCTCCCAGTGCAAGCAGACGTACTACTGCTCCACGGCCTG
  CCAGAGGAGAGACTGGTCTTCCCACAGCACCATCTGTAGGCCTGTACAGCAAAGTTTGAACAAGCTTGAA
  GATAATAAATCACCATTTGAAACAAAGGCCATTGAAGTGAAGAGTGAGGTTGACTGTCCCCCGGGAGTTA
  CTAAAGAAATAACAGCGGGTGCTGAGAGAGTAATGTTCTCTGATTTGAGAAGTCTCCAACTCAAGAAAAC
  CATGGAGATAAAGGGTACAGTTACTGAATTCAAGCACCCGAGTAACTTTTATATCCAGTTGTATTCTTCA
  GAGGTTCTAGAAAACATGAACCAACTCTCTACAAGCTTGAAAGAGACATATGCAAATGTGGTGCCTGAAG
  ATGGTTATCTTCCTGTTAAGGGGGAAGTTTGTGTTGCCAAATACACAGTTGATCAGACCTGGAACAGAGC
  CATAGTACAAGCCGTGGATGTGCTGCAGAGGAAGGCCCACGTCCTGTACATTGACTATGGGAACGAGGAG
  ATGATCCCGATAGACAGCGTTCACCCGCTGAGCAGAGGCCTTGACTTGTTTCCTCCTTCTGCCATAAAGT
  GCTGTGTGTCAGGCGTCATTCCCACTGCGGGCGAGTGGAGTGAAGGCTGTGTTGCAGCTGTCAAGGCCCT
  TCTGTTTGAGCAGTTCTGCTCTGTCAAGGTCATGGACATCTTAGAGGAGGAGGTACTCACCTGTGCCGTT
  GACCTTGTTCTACAGAGCTCAGGAAAGCAGCTGGACCATGTGCTGGTGGAAATGGGGTATGGAGTGAAAC
  CCGGTGAGCAGAGCTCCACGGAGCAGAGTGTGGACCACAGTGCATTGGAGGACGTTGGAAGAGTGACAGT
  TGAGAGCAAGATTGTGACAGACAGAAATGCCCTGATCCCCAAAGTGCTGACTTTGAATGTGGGTGATGAG
  TTCTGTGGCGTGGTTGCCCACATCCAGACACCAGAGGACTTCTTTTGTCAGCAGCTGCAGAGCGGCCACA
  AGCTTGCGGAGCTTCAGGAATCCCTCAGTGAATACTGTGGCCATGTGATTCCACGCTCTGACTTTTATCC
  AACCATTGGGGACGTGTGCTGTGCTCAGTTCTCAGAGGATGATCAGTGGTACCGCGCCTCGGTTCTGGCC
  TACGCTTCTGAAGAATCTGTCCTGGTTGGATATGTCGATTATGGGAACTTTGAGATTCTCAGTCTGAAAA
  GACTTTGTCCCATAATTCCAAAGTTGTTGGATTTGCCGATGCAAGCTCTAAATTGTGTGCTGGCAGGCGT
  GAAGCCATCATTAGGAATTTGGACTCCAGAAGCTGTGTGTGTCATGAAAGAGATGGTACAGAACAGGATG
  GTCACAGTGAGAGTGGTGGGCATGCTGGGGACCAGGGCCCTGGTGGAGCTCATCGACAAGTCGGTGGCTC
  CTCACGTCAGCGCTTCTAAAGCTCTCATAGACTCGGGCTTTGCCATCAAAGAAAAGGACGTGGCAGATAA
  AGGCAGCAGTATGCACACAGCCAGTGTTCCCTTGGCCATTGAAGGTCCAGCAGAGGCGTTGGAGTGGACG
  TGGGTGGAGTTCACTGTTGACGAGACCGTGGATGTGGTGGTCTGCATGATGTACAGTCCCGGGGAGTTCT
  ACTGCCACTTTCTTAAAGATGATGCCTTAGAGAAGCTCGATGACTTGAATCAGTCCTTAGCAGACTACTG
  TGCACAAAAGCCGCCCAATGGCTTTAAGGCAGAGATAGGGCGGCCTTGCTGTGCCTTTTTTTCAGGTGAC
  GGCAACTGGTACCGGGCTCTAGTCAAGGAGATCTTACCAAGTGGGAATGTTAAAGTCCACTTTGTGGATT
  ACGGAAATGTTGAAGAAGTTACCACAGACCAACTCCAGGCGATATTACCACAGTTCTTACTACTTCCATT
  TCAGGGGATGCAGTGCTGGCTAGTAGATATACAGCCCCCAAACAAGCATTGGACAAAAGAGGCCACAGCA
  AGATTTCAAGCATGTGTTGTGGGGCTCAAACTCCAAGCCAGAGTTGTGGAAATCACCGCGAACGGCGTGG
  GCGTGGAGCTCACCGATCTTTCCACTCCTTACCCCAAAATCATTAGTGATGTGCTCATCAGAGAGCAGTT
  GGTCTTAAGGTGTGGTTCACCACAGGACTCACTGATGAGCAGACCTGCTAATCAACATAAGCAGATCGAC
  AGCCACAGGGTGCAAGCCAGCCCTTCAGCAGAGCAGTGGAAGACAATGGAATTGCCAGTTAACAAGACTA
  TAGCAGCAAATGTACTAGAGATCATAAGCCCAGCCTTGTTCTACGCCATCCCCAGTGAAATGTCAGAAAA
  TCAAGAGAAGCTGTGTGTGTTAGCAGCTGAATTGTTAGAACACTGTAATGCTCAGAAGGGCCAGCCAGCC
  TACAGACCACGGACCGGCGACGCGTGCTGTGCTAAGTACACAAATGATGACTTCTGGTACCGGGCCATTG
  TTCTGGAAACGTCGGAATCTGATGTGAAAGTTCTCTACGCAGATTATGGAAACATCGAAACCCTGCCTCT
  TTCCAGAGTGCAGCCCATCCCAGCCAGCCACCTGGAGCTGCCCTTCCAGATCATTAGATGCTCACTAGAG
  GGGCCGATGGAGCTGAATGGAAGCTGTTCGCAGTTAGTGATGGAGCTGCTGAGAAATGCCATGCTGAACC
  AGAGTGTGGTTCTCTCTGTGAAAGCCATTTCAAAGAATGTCCACGCAGTGTCAGTTGAAAAATGTTCTGA
  GAACGGAATGATCAATATAGCTGAGAATCTGGTGATGTGTGGCCTGGCAGAAAACCTCACTTCTAAAAGG
  AAAAGTGCTTCCACTAAAGAGATACCACACAGCAGAGACTGCTGTTGCACAGAGTTACAGAAACAGATTG
  AGAAACACGAACAGATTCTCCTCTTCCTCTTAAACAATCCAACCAACCAAAGTAAATTCACAGAGATGAA
  AAAGCTGCTGAGAAGC
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226058 representing NM_031387
  Red=Cloning site Green=Tags(s)
  
  MMPRNNLEASTCKMAEPFNFEKKESKPPPQDPLRSPVAQHNHPTFRLKSPENGNTKNNFLLCEQNKQYLA
  SQEDSSVVSSNPAVVNGEVGGSKGDRKPPPTGNPVSPLSLGNSSPPNQVKTKPSSNVTPEKSKKSHKLFE
  NALSVNNPALFNSLGPPLRSTTCHRCGLFGSLRCSQCKQTYYCSTACQRRDWSSHSTICRPVQQSLNKLE
  DNKSPFETKAIEVKSEVDCPPGVTKEITAGAERVMFSDLRSLQLKKTMEIKGTVTEFKHPSNFYIQLYSS
  EVLENMNQLSTSLKETYANVVPEDGYLPVKGEVCVAKYTVDQTWNRAIVQAVDVLQRKAHVLYIDYGNEE
  MIPIDSVHPLSRGLDLFPPSAIKCCVSGVIPTAGEWSEGCVAAVKALLFEQFCSVKVMDILEEEVLTCAV
  DLVLQSSGKQLDHVLVEMGYGVKPGEQSSTEQSVDHSALEDVGRVTVESKIVTDRNALIPKVLTLNVGDE
  FCGVVAHIQTPEDFFCQQLQSGHKLAELQESLSEYCGHVIPRSDFYPTIGDVCCAQFSEDDQWYRASVLA
  YASEESVLVGYVDYGNFEILSLKRLCPIIPKLLDLPMQALNCVLAGVKPSLGIWTPEAVCVMKEMVQNRM
  VTVRVVGMLGTRALVELIDKSVAPHVSASKALIDSGFAIKEKDVADKGSSMHTASVPLAIEGPAEALEWT
  WVEFTVDETVDVVVCMMYSPGEFYCHFLKDDALEKLDDLNQSLADYCAQKPPNGFKAEIGRPCCAFFSGD
  GNWYRALVKEILPSGNVKVHFVDYGNVEEVTTDQLQAILPQFLLLPFQGMQCWLVDIQPPNKHWTKEATA
  RFQACVVGLKLQARVVEITANGVGVELTDLSTPYPKIISDVLIREQLVLRCGSPQDSLMSRPANQHKQID
  SHRVQASPSAEQWKTMELPVNKTIAANVLEIISPALFYAIPSEMSENQEKLCVLAAELLEHCNAQKGQPA
  YRPRTGDACCAKYTNDDFWYRAIVLETSESDVKVLYADYGNIETLPLSRVQPIPASHLELPFQIIRCSLE
  GPMELNGSCSQLVMELLRNAMLNQSVVLSVKAISKNVHAVSVEKCSENGMINIAENLVMCGLAENLTSKR
  KSASTKEIPHSRDCCCTELQKQIEKHEQILLFLLNNPTNQSKFTEMKKLLRS
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031387
• ORF Size: 3516 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031387.3, NP_113564.2
• RefSeq Size: 5047 bp
• RefSeq ORF: 3519 bp
• Locus ID: 83561
• UniProt ID: Q99MV1
• Cytogenetics: 19 D2
• Gene Summary: Plays a central role during spermatogenesis by participating in the repression transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Required for the localization of Piwi proteins to the meiotic nuage. Involved in the piRNA metabolic process by ensuring the entry of correct transcripts into the normal piRNA pool and limiting the entry of cellular transcripts into the piRNA pathway. May act by allowing the recruitment of piRNA biogenesis or loading factors that ensure the correct entry of transcripts and piRNAs into Piwi proteins.[UniProtKB/Swiss-Prot Function]