Kifc5b (NM_053173) Mouse Tagged ORF Clone

CAT#: MG225954

Kifc5b (tGFP-tagged) - Mouse kinesin family member C5B (Kifc5b), (10ug)

Product Images

Circular map for MG225954

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kifc5b
• Synonyms: Hset; Kifc5a; KNSL2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225954 representing NM_053173
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGCAGGCGCAGAGAAAGGGAAGGGAAGGGAAGAGGAACGTAGAACTGAAGGCGGCCCTGGTCA
  AGTCCTCCTCCCGACTGCCCCTGTCAGCAAGCAGCCTCAAGAGGGGTCCTGACCAGATGGAGGATGCCTT
  GGAGCCTGCAAAGAAACGGACACGAGTCATGGGTGCAGTGACCAAAGTTGACACATCCCGTCCCAGAGGA
  CCACTCCTCAGCACAGTGTCACAGACCCAGGGCCACAATGCAGCTCAGAAAGGCCCTAAGAAGACAGGAC
  CTGGTGGGTGCTCTGCTGTTGGTACAGTGCTGAGGAGCCAGAAGCCAGCTCCCGCTGCTCCTGCGAAGCC
  TGGCACATCCACTGCTCCTGTGGTGGTAGGGAAGAGAGCTGGCAAACGCCCTGCCTGGGACCTGAAGGGC
  CAGTTGTGTGACCTCAATGAAGAGTTGAAACGCTATCGGGAGAAGACTGAAACGCTGGAACTGGAGAACC
  GGGGTCTTCGGGAGCAACTCAGAGAGGTCCAGGAGCAGGCCACGACCCTGGGGACAGAGCGGAACACCCT
  GGAAGGGGAGCTGGCCAGTGTACGCAGCCGAGCTGAGCAGGACCAGCAGAGGCTGGAGACGCTGAGTGCC
  CGTGTCTTGGAGCTGGAGGAATGTCTGGGTACCAGGGAAAGGCTGCTTCAGGAGCTTCAGGGAGAGCGGC
  TGCAATTGCAGGAGGAGCGGAGCACACTGAGCACCCAACTGGAGGAGCAGGAGAGGAGGTTTCAGGCCAC
  AGAAGCAGCTCTGTCAAGCAGCCAAGAAGAGGTGGTGTGTCTTCGGCAGAAGACTGAAGCCCAGGTGACC
  TTACTGGCTGAGCAAGGAGACCGGCTCTATGGGTTAGAGATGGAGCGGCGGCGACTCCACAACCAGCTGC
  AGGAACTGAAGGGCAATATCCGGGTGTTCTGCCGCGTGCGCCCTGTCCTCGAAGGGGAATCCACTCCATC
  TCCTGGCTTCCTCGTGTTTCCTCCTGGCCCTGCTGGACCCTCTGATCGCCCGACGGGCCTTAGCCTCTCA
  CGATCTGATGATCGGCGCTCCACCCTGACTGGGGCCCCGGCACCCACTGTCCGCCATGATTTCTCCTTTG
  ATCGGGTGTTCCCGCCGGGAAGCAAGCAGGAGGAAGTGTTTGAGGAGATCGCCATGCTTGTCCAGTCAGC
  ACTAGATGGCTACCCTGTGTGCATTTTTGCCTATGGACAGACAGGCAGTGGCAAGACCTTCACTATGGAA
  GGAGGGCCTAGGGGAGACCCCCAATTGGCAGGGCTGATCCCTCGGGCCATGCGGCATCTGTTCTCTGTGG
  CCCAGGAGATGAGCGGCCAGGGCTGGACATACAGTTTTGTGGCGAGTTACGTAGAGATCTACAATGAGAC
  CGTTCGAGACCTGCTAGCTACTGGGCCCCGCAAGGGACAAGGGGGCGAGTGCGAGATCCGTCGGGCAAGC
  CCAGGGAGTGAGGAGCTTACTGTCACCAATGCCCGCTATGTCCCTGTTTCCTGTGAGAAAGAGGTGGAGG
  CCCTGCTCCATTTGGCTCACCAGAACCGGGCTGTGGCCCACACTGCCCAGAATAAGAGATCATCACGCAG
  TCATAGTGTGTTCCAGCTGCAGATTTCTGGAGAGCATGCAGCCCGGGGCCTGCAGTGTGGCGCTCCCCTC
  AACCTTGTGGACCTAGCTGGGAGTGAGCGGCTAGACCCTGGCTTACCCCTAGGCCCTGGGGAGCGTGATC
  GTCTTCGGGAGACACAGGCCATTAACAGCAGTCTGTCTACATTGGGACTGGTCATAATGGCCCTGAGCAA
  TAAGGAGTCCCACGTGCCTTACCGAAACAGCAAGCTCACCTACTTGCTGCAGAACTCTCTGGGTGGCAGT
  GCCAAGATGCTTATGTTTGTGAATATTTCTCCTCTGGAAGAGAATGTCTCCGAGTCTCTGAATTCACTAC
  GCTTTGCTTCCAAGGTGAACCAGTGTGTCATTGGTACTGCTCAGGCTAATAAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225954 representing NM_053173
  Red=Cloning site Green=Tags(s)
  
  MDVQAQRKGREGKRNVELKAALVKSSSRLPLSASSLKRGPDQMEDALEPAKKRTRVMGAVTKVDTSRPRG
  PLLSTVSQTQGHNAAQKGPKKTGPGGCSAVGTVLRSQKPAPAAPAKPGTSTAPVVVGKRAGKRPAWDLKG
  QLCDLNEELKRYREKTETLELENRGLREQLREVQEQATTLGTERNTLEGELASVRSRAEQDQQRLETLSA
  RVLELEECLGTRERLLQELQGERLQLQEERSTLSTQLEEQERRFQATEAALSSSQEEVVCLRQKTEAQVT
  LLAEQGDRLYGLEMERRRLHNQLQELKGNIRVFCRVRPVLEGESTPSPGFLVFPPGPAGPSDRPTGLSLS
  RSDDRRSTLTGAPAPTVRHDFSFDRVFPPGSKQEEVFEEIAMLVQSALDGYPVCIFAYGQTGSGKTFTME
  GGPRGDPQLAGLIPRAMRHLFSVAQEMSGQGWTYSFVASYVEIYNETVRDLLATGPRKGQGGECEIRRAS
  PGSEELTVTNARYVPVSCEKEVEALLHLAHQNRAVAHTAQNKRSSRSHSVFQLQISGEHAARGLQCGAPL
  NLVDLAGSERLDPGLPLGPGERDRLRETQAINSSLSTLGLVIMALSNKESHVPYRNSKLTYLLQNSLGGS
  AKMLMFVNISPLEENVSESLNSLRFASKVNQCVIGTAQANKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053173
• ORF Size: 2016 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053173.2, NP_444403.2
• RefSeq Size: 2627 bp
• RefSeq ORF: 2019 bp
• Locus ID: 16580
• UniProt ID: Q9QWT9
• Cytogenetics: 17 A3.3
• Gene Summary: Minus end-directed microtubule-dependent motor required for bipolar spindle formation (PubMed:16638812). May contribute to movement of early endocytic vesicles (PubMed:17360972). Regulates cilium formation and structure (PubMed:23807208).[UniProtKB/Swiss-Prot Function]