Sstr2 (NM_009217) Mouse Tagged ORF Clone

CAT#: MG225813

Sstr2 (tGFP-tagged) - Mouse somatostatin receptor 2 (Sstr2) transcript variant 2, (10ug)

Product Images

Circular map for MG225813

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sstr2
• Synonyms: Smstr-2; Smstr2; SRIF-1; SS2R; sst2; SSTR-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225813 representing NM_009217
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATGAGCTCTGAGCAGTTGAATGGGAGCCAAGTGTGGGTGTCCTCTCCATTTGACCTCAACGGCT
  CACTGGGGCCAAGCAATGGCTCCAACCAGACCGAGCCATACTACGACATGACAAGCAACGCCGTCCTCAC
  GTTCATCTACTTCGTGGTGTGTGTTGTCGGGCTGTGCGGCAACACGCTGGTCATTTATGTCATCCTCCGC
  TATGCCAAGATGAAGACCATCACCAACATCTACATCCTTAACCTGGCCATTGCAGATGAACTCTTCATGC
  TAGGGCTGCCCTTCTTGGCCATGCAGGTGGCGCTAGTCCACTGGCCTTTTGGCAAGGCCATCTGCCGGGT
  GGTCATGACTGTAGATGGCATCAATCAGTTCACCAGTATCTTCTGCTTGACGGTCATGAGCATCGACCGC
  TACCTGGCCGTGGTGCACCCCATTAAGTCAGCCAAATGGAGGCGACCCCGGACAGCCAAGATGATCAATG
  TAGCTGTGTGGTGTGTGTCTCTGCTCGTCATTTTGCCCATCATGATATACGCCGGCCTCCGGAGCAACCA
  GTGGGGCAGGAGCAGCTGTACCATCAACTGGCCAGGCGAATCCGGGGCGTGGTACACAGGTTTCATTATC
  TACGCCTTCATCCTGGGGTTCCTGGTACCCCTTACCATCATTTGTCTCTGCTACCTGTTCATCATCATCA
  AGGTGAAGTCCTCTGGAATCCGAGTGGGATCATCCAAGAGGAAAAAGTCAGAGAAAAAGGTGACCCGCAT
  GGTGTCCATCGTAGTGGCTGTCTTCATCTTCTGCTGGCTCCCCTTCTACATCTTCAACGTCTCTTCCGTG
  TCTGTGGCCATCAGTCCCACCCCAGCCCTGAAAGGCATGTTTGACTTTGTGGTGATCCTCACCTATGCCA
  ACAGCTGCGCCAACCCCATCCTGTACGCCTTCTTGTCTGACAACTTCAAGAAGAGCTTCCAGAATGTTCT
  TTGCTTGGTCAAGGCAGACAATTCACAATCCGGAGCGGAAGACATCATTGCCTGGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225813 representing NM_009217
  Red=Cloning site Green=Tags(s)
  
  MEMSSEQLNGSQVWVSSPFDLNGSLGPSNGSNQTEPYYDMTSNAVLTFIYFVVCVVGLCGNTLVIYVILR
  YAKMKTITNIYILNLAIADELFMLGLPFLAMQVALVHWPFGKAICRVVMTVDGINQFTSIFCLTVMSIDR
  YLAVVHPIKSAKWRRPRTAKMINVAVWCVSLLVILPIMIYAGLRSNQWGRSSCTINWPGESGAWYTGFII
  YAFILGFLVPLTIICLCYLFIIIKVKSSGIRVGSSKRKKSEKKVTRMVSIVVAVFIFCWLPFYIFNVSSV
  SVAISPTPALKGMFDFVVILTYANSCANPILYAFLSDNFKKSFQNVLCLVKADNSQSGAEDIIAWV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009217
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009217.5
• RefSeq Size: 1041 bp
• RefSeq ORF: 1041 bp
• Locus ID: 20606
• UniProt ID: P30875
• Cytogenetics: 11 79.05 cM
• Gene Summary: The protein encoded by this gene is a receptor for somatostatin, which acts at many sites to inhibit the release of several hormones and other secretory proteins. The encoded protein is a member of the superfamily of receptors having seven transmembrane segments and is involved in many processes, including adenylyl cyclase inhibition, phosphotyrosine phosphatase stimulation, and inhibition of calcium entry and cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]