Fam177a (NM_028527) Mouse Tagged ORF Clone
CAT#: MG225664
- TrueORF®
Fam177a (tGFP-tagged) - Mouse family with sequence similarity 177 member A (Fam177a), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_028527" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Fam177a |
Synonyms | 1700047I17Rik; 1700047I17Rik1; C14orf24; D730001C10Rik; Fam177a1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225664 representing NM_028527
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGTGAGTCGGGATGCGCGGCGGCGGGAGAGACCGAGGCAGCGGCCGCCACGGCGTTCCGAGACG CGACACGGCAGATAAGTAATGAAAGAGGATTTGAGAATGTAGAACTGGGCGTCATGGGGAAGAAGAAGAA AGTCCCGAGGAGGGTCATCCACTTTGTCAGTGGAGAGACAATGGAGGAGTACAGCACCGACGAGGACGAG GTGGACGGCCTGGACAAGAAGGACGTGCTGCCCACTGTCGACCCGACAAAACTTACCTGGGGCCCCTACC TATGGTTTTACATGCTGCGGGCCGCCACATCAACCCTCTCAGTGTGTGACTTTCTTGGAGAGAAGATTGC GTCAGTTTTGGGCATCAGCACCCCAAAATACCAGTACGCCATTGATGAGTATTACCGGATGAAGAAGGAG GAGGAGGAGGAGGAAGAAGAAAATAGGATGTCAGAAGAAGCAGAAAGACAATACCAACAAAACAAGCTGC AGGCCGATTCCATTGTACAGACAGACCAACCAGAAACAGTGTCGTCCAGCTTTGTAAATATTAATTTTGA AATGGAGGAAGACTGTGAAGCAATTAAGGAAAACAAACAGCGTCCAGTCTCTGTCCCACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225664 representing NM_028527
Red=Cloning site Green=Tags(s) MERESGCAAAGETEAAAATAFRDATRQISNERGFENVELGVMGKKKKVPRRVIHFVSGETMEEYSTDEDE VDGLDKKDVLPTVDPTKLTWGPYLWFYMLRAATSTLSVCDFLGEKIASVLGISTPKYQYAIDEYYRMKKE EEEEEEENRMSEEAERQYQQNKLQADSIVQTDQPETVSSSFVNINFEMEEDCEAIKENKQRPVSVPP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_028527 |
ORF Size | 621 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028527.2 |
RefSeq Size | 3863 bp |
RefSeq ORF | 624 bp |
Locus ID | 73385 |
UniProt ID | Q8BR63 |
Cytogenetics | 12 C1 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211418 | Fam177a (untagged) - Mouse family with sequence similarity 177, member A (Fam177a), (10ug) |
USD 330.00 |
|
MR225664 | Fam177a (Myc-DDK-tagged) - Mouse family with sequence similarity 177, member A (Fam177a) |
USD 300.00 |
|
MR225664L3 | Lenti ORF clone of Fam177a (Myc-DDK-tagged) - Mouse family with sequence similarity 177, member A (Fam177a) |
USD 600.00 |
|
MR225664L4 | Lenti ORF clone of Fam177a (mGFP-tagged) - Mouse family with sequence similarity 177, member A (Fam177a) |
USD 600.00 |
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