Prom1 (NM_001163581) Mouse Tagged ORF Clone

CAT#: MG225619

Prom1 (tGFP-tagged) - Mouse prominin 1 (Prom1) transcript variant 4, (10ug)

Product Images

Circular map for MG225619

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prom1
• Synonyms: 4932416E19Rik; AC133; CD133; Prom; Prom-1; Proml1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225619 representing NM_001163581
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCGTCTTCAGTGCCCTGCTGTTACTGGGGCTGTGTGGAAAGATCTCTTCAGAAGGTCAGCCTG
  CATTCCATAACACTCCTGGGGCTATGAATTATGAATTGCCTACCACCAAATATGAGACCCAAGATACCTT
  CAATGCTGGGATTGTTGGCCCTCTCTACAAAATGGTGCACATCTTCCTCAACGTGGTCCAGCCGAATGAC
  TTCCCTCTAGATTTGATCAAAAAACTCATACAGAACAAGAACTTTGACATCTCAGTTGATTCCAAGGAGA
  TCGGAGTCCTTATCTGCGCCATCCTGGGACTGCTGTTCATTATCCTCATGCCTCTGGTGGGCTGCTTCTT
  TTGTATGTGCCGTTGCTGCAACAAATGCGGCGGAGAGATGCACCAGCGGCAGAAGCAGAATGCGCCATGC
  AGGAGGAAGTGCTTGGGCCTCTCCCTCCTGGTGATTTGTCTGCTCATGAGCCTTGGCATTATATATGGCT
  TTGTGGCTAACCAGCAGACCAGGACTCGGATCAAAGGGACCCAGAAACTGGCAAAGAGCAATTTCAGAGA
  CTTTCAAACACTCCTGACTGAAACACCAAAGCAAATTGACTATGTAGTGGAGCAGTACACCAACACCAAG
  AACAAGGCATTCTCAGACCTGGATGGCATCGGCTCCGTGCTGGGAGGCAGAATAAAGGACCAACTAAAAC
  CCAAAGTAACTCCTGTCCTCGAAGAGATTAAGGCCATGGCGACAGCCATCAAACAGACCAAGGATGCCCT
  GCAGAACATGAGCAGCAGCCTGAAAAGTCTCCAAGATGCAGCCACCCAGCTCAATACCAACCTGAGCTCT
  GTGAGAAACAGCATCGAGAATTCGCTCAGCAGCAGTGACTGTACCTCAGATCCAGCCAGCAAGATCTGCG
  ATAGCATCAGACCAAGCCTAAGCAGTCTGGGGAGCAGCCTCAATTCAAGTCAGGGGTATACGACAATTGA
  TGAAATACCCAATACAATACAAAACCAAACTGTGGATGTCATCAAAGACGTCAAAAATACCTTGGACTCC
  ATTAGCTCCAACATTAAGGACATGAGCCAAAGTATTCCTATTGAGGATATGCTGTTACAGGTCTCCCATT
  ACCTTAATAACAGCAACAGATACTTAAACCAGGAGCTGCCCAAGCTGGAAGAATATGACTCGTACTGGTG
  GCTGGGTGGCTTGATTGTCTGCTTTCTGCTGACTCTCATTGTGACCTTCTTTTTCCTGGGCTTGCTGTGT
  GGTGTGTTTGGCTATGACAAGCATGCCACCCCAACTAGAAGAGGCTGTGTGTCCAACACTGGAGGCATCT
  TCCTCATGGCTGGGGTTGGATTCGGCTTCCTTTTTTGCTGGATATTGATGATCCTTGTGGTTCTTACGTT
  TGTTGTTGGTGCAAATGTGGAAAAGTTGCTCTGCGAACCTTATGAAAACAAGAAATTATTACAGGTTTTG
  GACACTCCCTATCTGCTCAAGGAACAATGGCAATTTTATCTTTCTGGCATGCTATTCAATAACCCAGACA
  TTAACATGACCTTTGAGCAAGTCTACAGGGATTGCAAAAGAGGTCGAGGTATATATGCTGCTTTTCAGCT
  TGAGAATGTCGTCAACGTCAGTGATCATTTCAACATTGACCAGATTTCTGAAAACATAAATACGGAGTTG
  GAAAACCTGAATGTGAACATTGATAGCATTGAACTGTTGGATAACACAGGAAGGAAGAGCCTCGAGGACT
  TTGCACATTCTGGGATAGATACAATCGATTATTCCACATACTTGAAGGAGACTGAGAAATCCCCTACTGA
  AGTGAATCTGCTGACATTTGCCTCTACCCTGGAAGCAAAAGCAAACCAGTTGCCTGAAGGAAAGCTGAAA
  CAGGCCTTCTTACTGGATGTACAGAATATAAGAGCCATCCACCAGCATCTCCTCCCTCCTGTGCAGCAAT
  CACTGAATACGTTAAGACAAAGTGTCTGGACCCTCCAGCAAACAAGCAACAAGTTGCCGGAGAAAGTGAA
  GAAGATCCTTGCCTCTTTGGACTCTGTTCAGCATTTCCTCACCAATAACGTTTCCCTCATCGTTATCGGG
  GAAACGAAGAAGTTTGGGAAAACAATACTAGGCTACTTTGAACATTATCTGCACTGGGTCTTTTATGCCA
  TCACAGAGAAGATGACATCCTGCAAACCCATGGCCACCGCGATGGACTCTGCTGTTAATGGCATTCTGTG
  TGGCTATGTTGCGGACCCTCTGAATTTGTTCTGGTTCGGCATAGGGAAAGCCACGGTGCTCTTACTTCCG
  GCTGTAATCATTGCTATCAAGCTGGCCAAGTACTATCGCAGGATGGATTCAGAGGATGTATACGACGACT
  CGTCCGTCTCGGGGATGTGGCATTTCACTTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225619 representing NM_001163581
  Red=Cloning site Green=Tags(s)
  
  MALVFSALLLLGLCGKISSEGQPAFHNTPGAMNYELPTTKYETQDTFNAGIVGPLYKMVHIFLNVVQPND
  FPLDLIKKLIQNKNFDISVDSKEIGVLICAILGLLFIILMPLVGCFFCMCRCCNKCGGEMHQRQKQNAPC
  RRKCLGLSLLVICLLMSLGIIYGFVANQQTRTRIKGTQKLAKSNFRDFQTLLTETPKQIDYVVEQYTNTK
  NKAFSDLDGIGSVLGGRIKDQLKPKVTPVLEEIKAMATAIKQTKDALQNMSSSLKSLQDAATQLNTNLSS
  VRNSIENSLSSSDCTSDPASKICDSIRPSLSSLGSSLNSSQGYTTIDEIPNTIQNQTVDVIKDVKNTLDS
  ISSNIKDMSQSIPIEDMLLQVSHYLNNSNRYLNQELPKLEEYDSYWWLGGLIVCFLLTLIVTFFFLGLLC
  GVFGYDKHATPTRRGCVSNTGGIFLMAGVGFGFLFCWILMILVVLTFVVGANVEKLLCEPYENKKLLQVL
  DTPYLLKEQWQFYLSGMLFNNPDINMTFEQVYRDCKRGRGIYAAFQLENVVNVSDHFNIDQISENINTEL
  ENLNVNIDSIELLDNTGRKSLEDFAHSGIDTIDYSTYLKETEKSPTEVNLLTFASTLEAKANQLPEGKLK
  QAFLLDVQNIRAIHQHLLPPVQQSLNTLRQSVWTLQQTSNKLPEKVKKILASLDSVQHFLTNNVSLIVIG
  ETKKFGKTILGYFEHYLHWVFYAITEKMTSCKPMATAMDSAVNGILCGYVADPLNLFWFGIGKATVLLLP
  AVIIAIKLAKYYRRMDSEDVYDDSSVSGMWHFTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163581
• ORF Size: 2412 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163581.1, NP_001157053.1
• RefSeq Size: 2638 bp
• RefSeq ORF: 2415 bp
• Locus ID: 19126
• UniProt ID: O54990
• Cytogenetics: 5 B3
• Gene Summary: May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.[UniProtKB/Swiss-Prot Function]