Anxa11 (NM_013469) Mouse Tagged ORF Clone

CAT#: MG225524

Anxa11 (tGFP-tagged) - Mouse annexin A11 (Anxa11), (10ug)

Product Images

Circular map for MG225524

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Anxa11
• Synonyms: A830099O17Rik; Anx11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225524 representing NM_013469
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACCCAGGCTACCCTCCACCTGCCGGTGGCTACCCTCCAGCTGCACCAGGTGGTGGTCCCTGGG
  GAGGTGCTGGCTACCCTCCTCCCAGCATGCCCCCTATCGGGCTGGATAACGTAGCCAACTATGCGGGGCA
  GTTCAACCAGGACTACCTCTCAGGCATGGCAGCCAACATGTCTGGAACATTTGGAGGAGCCAACGTGCCA
  AATCTGTATCCTGGGGCTCCTGGGGGTGGCTATCCTCCAGTCCCTCCTGGTGGCTTTGGGCAGCCCCCTC
  CTGCCCAGCAGCCTGTCCCTCCTTATGGAATGTATCCGCCCCCAGGAGGAAACCCACCTCCTGGGATGCC
  TTCATATCCAGCCTACCCAGGGGCCCCTGTGCCAGGCCAGCCTATGCCACCCACTGGGCAGCAGCCCCCA
  GGGGCCTATCCTGGGCAGCCTCCAATGACCTATCCTGGGCAGTCACCAATGCCACCCCCTGGGCAGCAGC
  CAGTGCCAAGTTACCCAGGATACTCGGGATCTAGTACCATCACCCCTGCTGTGCCACCAGCCCAGTTTGG
  AAACCGAGGTACCATCACTGCTGCTTCTGGATTTGACCCCTTGCGAGATGCCGAGGTCCTGCGCAAGGCT
  ATGAAGGGCTTTGGAACGGATGAGCAGGCCATCATCGACTGCCTGGGGAGCCGGTCCAACAAGCAGCGTC
  AGCAGATCCTCCTATCCTTCAAGACGGCCTATGGCAAGGATTTGATCAAAGACCTAAAATCAGAACTGTC
  GGGAAACTTTGAGAAGACTATCCTGGCCCTGATGAAGACCCCGGTCCTGTTTGATGTCTATGAGATAAAG
  GAAGCTATCAAGGGGGCTGGTACTGATGAGGCCTGCCTGATTGAGATCTTCGCTTCCAGAAGTAACGAAC
  ACATCCGGGAGCTAAGCAGGGCCTACAAAACAGAGTTCCAAAAAACTCTGGAGGAGGCCATTCGAAGCGA
  CACATCAGGACACTTCCAGCGGCTCCTCATCTCTCTCTCTCAGGGAAACCGTGATGAGAGCACAAATGTG
  GACATGTCCCTTGTCCAGAGAGATGTCCAGGAGCTGTATGCAGCTGGGGAGAACCGCCTGGGAACAGACG
  AGTCCAAGTTCAACGCCATCCTGTGCTCCCGGAGCCGGGCCCACCTGGTGGCAGTTTTCAACGAGTATCA
  GAGGATGACGGGCCGAGACATTGAGAAGAGCATCTGCCGGGAGATGTCTGGGGACCTGGAGCAGGGCATG
  CTGGCTGTGGTGAAATGCCTCAAGAACACCCCCGCCTTCTTTGCTGAAAGGCTCAACAAGGCTATGAGGG
  GAGCAGGAACAAAGGACCGGACCCTGATTCGCATCATGGTGTCTCGCAGCGAGCTTGACCTGCTGGATAT
  CCGAGCAGAGTATAAGCGGATGTACGGCAAGTCACTGTACCACGATATCACGGGAGATACTTCTGGAGAC
  TATCGAAAGATTCTACTGAAGATCTGTGGTGGCAACGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225524 representing NM_013469
  Red=Cloning site Green=Tags(s)
  
  MSYPGYPPPAGGYPPAAPGGGPWGGAGYPPPSMPPIGLDNVANYAGQFNQDYLSGMAANMSGTFGGANVP
  NLYPGAPGGGYPPVPPGGFGQPPPAQQPVPPYGMYPPPGGNPPPGMPSYPAYPGAPVPGQPMPPTGQQPP
  GAYPGQPPMTYPGQSPMPPPGQQPVPSYPGYSGSSTITPAVPPAQFGNRGTITAASGFDPLRDAEVLRKA
  MKGFGTDEQAIIDCLGSRSNKQRQQILLSFKTAYGKDLIKDLKSELSGNFEKTILALMKTPVLFDVYEIK
  EAIKGAGTDEACLIEIFASRSNEHIRELSRAYKTEFQKTLEEAIRSDTSGHFQRLLISLSQGNRDESTNV
  DMSLVQRDVQELYAAGENRLGTDESKFNAILCSRSRAHLVAVFNEYQRMTGRDIEKSICREMSGDLEQGM
  LAVVKCLKNTPAFFAERLNKAMRGAGTKDRTLIRIMVSRSELDLLDIRAEYKRMYGKSLYHDITGDTSGD
  YRKILLKICGGND
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013469
• ORF Size: 1509 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013469.2, NP_038497.2
• RefSeq Size: 2413 bp
• RefSeq ORF: 1512 bp
• Locus ID: 11744
• UniProt ID: P97384
• Cytogenetics: 14 15.06 cM
• Gene Summary: Required for midbody formation and completion of the terminal phase of cytokinesis (By similarity). Binds specifically to calcyclin in a calcium-dependent manner.[UniProtKB/Swiss-Prot Function]