Trabd2b (NM_001085549) Mouse Tagged ORF Clone

CAT#: MG225412

Trabd2b (tGFP-tagged) - Mouse predicted gene 12824 (Gm12824), (10ug)

Product Images

Circular map for MG225412

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trabd2b
• Synonyms: Gm12824; Hkat; OTTMUSG00000008561
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225412 representing NM_001085549
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGCCGCCCTGGCGGGGCCGCTGCTAGCCGCCCTCCTTGCCACCGCCCGCGCCCGTCCGCAACCCC
  CCGACGGAGGACAGTGCCGGCCGCCTGGATCGCAGAGGGACCTGAACTCCTTCCTGTGGACTATTCGGCG
  CCATCCTCCAGCCTACTTGTTTGGCACCATCCACGTTCCCTATACCCGAGTCTGGGACTTCATCCCTGAT
  AATTCCAAAGCAGCCTTCCAGGCCAGCACTCACGTCTACTTTGAGTTGGATCTGACAGATCCCTATACCA
  TCTCAGCCCTGGCCAGTTGCCAGCTGCTGCCCCATGGGGAGAACCTGCAGGACGTGCTGCCAAGGGAGCT
  CTACTGGCGCCTGAAGCGCCACCTGGACTATGTGAAGCTGATGATCCCCTCCTGGATGACGCCAGCACAG
  CGCGGCAAGGGGCTGTATGCTGACTACCTGTTCAATGCCATTGCTGGTAACTGGGAGCGCAAGAGGCCCG
  TGTGGGTGATGCTCATGGTGAACTCCCTCACGGAGACAGATGTGCGCTCCCGAGGTGTGCCTGTGCTGGA
  CCTCTACCTGGCCCAGCAGGCTGAGAAGATGAAGAAGAGCACAGGGGCTGTGGAGCGGGTTGAGGAGCAG
  TGCCATCCTCTCAATGGGCTCAACTTCTCCCAGGTGTTGTTTGCCCTGAACCAGACTCTGCTGCAGCACG
  AAAGTGTGCGGGCTGGGAGCCTGCAGGCACCCTACACCACAGAGGACCTCATCAAACACTACAACTGCGG
  GGACCTCAATGCTGTCATCTTCAACCATGACACATCCCAGCTACCCAACTTCATCAACACCACCCTCCCA
  CCTCATGAGCAGGTGACAGCCCAGGAAATCGACAGCTACTTCCGGCAGGAGCTCATCTACAAGAGGAATG
  AGCGCATGGGAAAGAGGGTCATGGCGCTGCTGCAGGAAAACCAGGACAAGATCTGCTTCTTTGCCTTCGG
  AGCAGGTCACTTTCTGGGGAACAACACTGTCATCGATGTCCTAAGACAGGCAGGGCTGGAGGTGGACCAC
  ACACCTGCTGGGCAAGCCATCCACGGTCCTGCTGCTGTTGGAAGCCCAGCTCCCCCTCCTGAGATCACCT
  CTCCTGCCAGCCCAGCTCCAGCAACCCCAGCCGCTGCAGTCCCAGAAGCAACCTCAGCGACCCCCACCAC
  CCCTCCTGAGGAGGAAGACCCAGTCCTGTCCCCACACCTCTTGCTCCCCGACAGCCTAAGCCAGCTAGAG
  GAGTTCGGAAGGCAGAAGTGGCGAAAGAGACTAAATAAACATCAGCGGCCAAGGCAGTTCAATGACCTCT
  GGGTCCGAATCGAGGACAGCACTACGATCTCGCCACCTCCGCTGCCCCTGCAGCCTACCCCCAGCTCCGA
  AACCACCAAGCCCTTTGTCAAGTCCTCACACCAACTCCAGCAGCAGGACGCTGTGGGGCCTACCAGCAGC
  TCAGCGCCTACACTGGGCCTCCTCCATGCCATCACTGCCTCCATTGTAGCCCCCTTCCTACTACACAGCC
  TTGGGCCTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225412 representing NM_001085549
  Red=Cloning site Green=Tags(s)
  
  MHAALAGPLLAALLATARARPQPPDGGQCRPPGSQRDLNSFLWTIRRHPPAYLFGTIHVPYTRVWDFIPD
  NSKAAFQASTHVYFELDLTDPYTISALASCQLLPHGENLQDVLPRELYWRLKRHLDYVKLMIPSWMTPAQ
  RGKGLYADYLFNAIAGNWERKRPVWVMLMVNSLTETDVRSRGVPVLDLYLAQQAEKMKKSTGAVERVEEQ
  CHPLNGLNFSQVLFALNQTLLQHESVRAGSLQAPYTTEDLIKHYNCGDLNAVIFNHDTSQLPNFINTTLP
  PHEQVTAQEIDSYFRQELIYKRNERMGKRVMALLQENQDKICFFAFGAGHFLGNNTVIDVLRQAGLEVDH
  TPAGQAIHGPAAVGSPAPPPEITSPASPAPATPAAAVPEATSATPTTPPEEEDPVLSPHLLLPDSLSQLE
  EFGRQKWRKRLNKHQRPRQFNDLWVRIEDSTTISPPPLPLQPTPSSETTKPFVKSSHQLQQQDAVGPTSS
  SAPTLGLLHAITASIVAPFLLHSLGPS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001085549
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001085549.1, NP_001079018.1
• RefSeq Size: 6617 bp
• RefSeq ORF: 1554 bp
• Locus ID: 666048
• UniProt ID: B1ATG9
• Cytogenetics: 4
• Gene Summary: Metalloprotease that acts as a negative regulator of the Wnt signaling pathway by mediating the cleavage of the 8 N-terminal residues of a subset of Wnt proteins. Following cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation. Able to cleave WNT3A, WNT5, but not WNT11. Required for head formation (By similarity).[UniProtKB/Swiss-Prot Function]