Trpm2 (NM_138301) Mouse Tagged ORF Clone

CAT#: MG225380

Trpm2 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 2 (Trpm2), (10ug)

Product Images

Circular map for MG225380

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trpm2
• Synonyms: 9830168K16Rik; C79133; LTRPC2; Trp7; TRPC7; Trrp7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225380 representing NM_138301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCTTGGACCGGAGAAGAACTGGCTCTGAGCAGGAGGAGGGCTTTGGGGTGCAGTCAAGGAGGG
  CCACTGACCTGGGCATGGTCCCCAATCTCCGACGAAGCAATAGCAGCCTTTGCAAGAGCAGGAGATTTCT
  GTGCTCTTTCAGCAGTGAGAAGCAAGAAAACCTTAGCTCATGGATTCCCGAGAATATCAAGAAGAAGGAG
  TGTGTGTACTTCGTGGAAAGTTCCAAACTCTCGGACGCAGGGAAGGTAGTGTGTGCGTGTGGTTATACCC
  ACGAGCAACACTTGGAGGTGGCCATCAAGCCACACACCTTCCAGGGCAAGGAGTGGGATCCAAAGAAACA
  CGTCCAAGAGATGCCCACAGATGCCTTTGGTGACATCGTTTTCACAGACCTGAGCCAGAAAGTGGGGAAG
  TATGTCCGGGTCTCCCAGGACACGCCCTCCAGTGTCATCTACCAGCTCATGACCCAGCACTGGGGCCTAG
  ATGTCCCCAACCTCCTCATCTCCGTGACCGGTGGGGCCAAGAACTTCAACATGAAGCTGAGGCTGAAGAG
  CATCTTCCGGAGAGGCTTGGTCAAGGTGGCTCAAACCACGGGGGCCTGGATCATCACTGGAGGATCCCAC
  ACAGGCGTGATGAAGCAGGTGGGCGAAGCGGTACGGGACTTCAGTCTGAGCAGCAGCTGCAAAGAAGGTG
  AAGTCATCACTATTGGCGTAGCCACGTGGGGCACCATCCACAACCGCGAGGGACTGATCCATCCCATGGG
  AGGCTTCCCCGCCGAGTACATGCTGGATGAGGAAGGCCAAGGGAACCTGACCTGCTTGGACAGCAACCAT
  TCCCACTTCATCTTGGTGGATGATGGGACCCACGGGCAATATGGTGTGGAGATTCCGCTGAGGACTAAGC
  TGGAAAAGTTCATCTCAGAGCAAACGAAGGAAAGGGGAGGTGTGGCCATCAAGATCCCCATTGTCTGCGT
  GGTGTTGGAGGGTGGCCCTGGCACTCTGCATACAATCTACAATGCCATCAACAATGGCACACCCTGCGTG
  ATAGTGGAGGGCTCTGGCCGAGTGGCTGACGTCATCGCTCAGGTAGCTACTCTGCCTGTCTCTGAGATCA
  CCATCTCCTTGATCCAGCAGAAGCTCAGCATATTCTTCCAGGAGATGTTTGAGACTTTCACCGAAAACCA
  GATTGTGGAATGGACCAAAAAGATCCAAGACATTGTCCGGAGGCGGCAGCTGCTGACGATCTTCCGGGAA
  GGCAAGGATGGTCAGCAGGATGTGGATGTGGCCATTCTGCAGGCGTTACTGAAAGCCTCTCGAAGCCAAG
  ACCACTTTGGCCACGAGAACTGGGACCACCAACTGAAGTTGGCTGTGGCCTGGAACCGCGTGGACATCGC
  TCGCAGTGAGATCTTCACCGATGAATGGCAGTGGAAGCCTGCAGATCTGCATCCCATGATGACGGCCGCT
  CTCATCTCCAACAAGCCTGAGTTCGTGAGGCTCTTTCTGGAGAATGGGGTGCGGCTCAAGGAGTTTGTCA
  CTTGGGATACTCTTCTCTGCCTGTACGAGAACCTGGAGCCGTCCTGTCTCTTCCACAGCAAGCTACAGAA
  GGTGCTGGCCGAAGAACAGCGCTTAGCCTATGCATCTGCAACACCCCGCCTGCACATGCACCATGTGGCC
  CAGGTGCTTCGTGAACTCCTGGGGGACTCCACGCAGCTGCTGTACCCCCGGCCCCGGTACACTGACAGGC
  CACGGCTCTCGATGACCGTGCCACACATCAAGCTGAACGTGCAGGGAGTGAGCCTCCGGTCCCTCTATAA
  GCGATCAACAGGCCACGTTACCTTCACCATTGACCCAGTCCGTGACCTTCTCATTTGGGCCGTTATCCAG
  AACCACAGGGAGCTGGCAGGCATCATCTGGGCTCAGAGTCAGGACTGTACTGCCGCAGCACTGGCCTGTA
  GCAAGATCCTGAAGGAGCTGTCCAAGGAGGAGGAAGATACAGACAGCTCTGAGGAGATGCTGGCACTGGC
  AGACGAGTTTGAGCACAGAGCTATAGGCGTCTTCACTGAGTGCTACAGGAAGGATGAGGAAAGAGCCCAG
  AAGCTGCTTGTCCGTGTGTCTGAGGCCTGGGGGAAGACCACCTGCCTGCAGCTGGCCCTAGAGGCCAAGG
  ACATGAAATTCGTGTCTCATGGAGGCATCCAGGCTTTCCTAACCAAGGTGTGGTGGGGCCAGCTCTGTGT
  GGACAATGGCCTGTGGAGGATCATCCTGTGCATGCTGGCCTTCCCGCTGCTCTTCACCGGCTTCATCTCC
  TTCAGGGAAAAGAGGCTGCAGGCACTGTGTCGCCCAGCCCGCGTCCGCGCCTTCTTCAATGCGCCTGTGG
  TCATCTTCCACATGAATATCCTCTCCTACTTTGCCTTCCTCTGCCTGTTCGCCTACGTGCTCATGGTGGA
  CTTCCAGCCTTCTCCATCCTGGTGCGAGTACCTCATCTACCTGTGGCTCTTCTCCCTGGTGTGCGAAGAG
  ACTCGGCAGCTATTCTATGATCCTGATGGCTGTGGACTAATGAAGATGGCGTCCCTGTACTTCAGTGACT
  TCTGGAACAAACTGGACGTTGGGGCCATTCTGCTCTTCATAGTAGGACTGACCTGTCGGCTCATCCCAGC
  GACGCTGTACCCTGGGCGCATCATCCTGTCTTTGGACTTCATCATGTTCTGTCTCCGTCTCATGCACATC
  TTCACTATTAGCAAGACACTGGGGCCCAAGATAATCATCGTGAAGCGGATGATGAAGGACGTCTTCTTCT
  TCCTCTTTCTCCTGGCGGTGTGGGTGGTGTCCTTCGGCGTAGCTAAGCAGGCCATTCTCATACATAACGA
  GAGCCGCGTGGACTGGATCTTCCGTGGGGTTGTCTATCACTCTTACCTGACCATCTTTGGCCAGATCCCA
  ACCTACATTGACGGTGTGAATTTCAGCATGGACCAGTGCAGCCCCAATGGCACGGACCCCTACAAGCCTA
  AGTGTCCTGAGAGCGACTGGACGGGACAGGCACCTGCCTTCCCCGAGTGGCTGACTGTCACCCTGCTCTG
  CCTCTACCTGCTCTTTGCCAACATCCTGCTGCTTAACCTGCTCATCGCCATGTTCAACTACACCTTCCAG
  GAGGTGCAGGAACACACAGACCAGATCTGGAAATTCCAGCGCCACGACCTGATCGAGGAGTACCATGGCC
  GTCCCCCGGCACCTCCCCCACTCATCCTCCTCAGCCACCTGCAGCTCCTGATCAAGAGGATTGTCCTGAA
  GATCCCTGCCAAGAGGCATAAGCAGCTCAAGAACAAGCTGGAGAAGAACGAGGAGACAGCGCTCCTGTCT
  TGGGAACTGTACCTGAAGGAGAACTACCTGCAGAACCAGCAGTACCAGCAGAAACAGCGTCCAGAGCAGA
  AAATCCAAGACATCAGTGAGAAAGTGGACACCATGGTGGATCTGCTGGACATGGACCAGGTGAAGAGGTC
  AGGCTCCACAGAGCAGAGACTGGCTTCCCTGGAGGAACAGGTGACTCAGGTGACCAGAGCTTTGCACTGG
  ATCGTGACAACCCTGAAGGACAGTGGCTTTGGCTCAGGAGCAGGTGCGCTGACCCTGGCACCCCAGAGGG
  CCTTCGATGAGCCAGATGCTGAGCTGAGTATCAGGAGGAAAGTAGAGGAACCAGGAGATGGTTACCACGT
  GAGCGCCCGGCATCTCCTCTATCCCAATGCCCGCATCATGCGCTTCCCCGTGCCTAACGAGAAGGTGCCT
  TGGGCGGCAGAGTTTCTGATCTACGATCCTCCCTTTTACACCGCTGAGAAGGATGTGGCTCTCACAGACC
  CCGTGGGAGACACTGCAGAACCTCTGTCTAAGATCAGTTACAACGTCGTGGATGGACCGACGGACCGTCG
  CAGCTTCCATGGAGTCTACGTGGTCGAGTATGGGTTCCCGTTGAACCCCATGGGCCGCACAGGGTTGCGT
  GGTCGTGGGAGCCTCAGCTGGTTTGGTCCCAACCACACTCTGCAGCCAGTTGTCACCCGGTGGAAGAGGA
  ACCAGGGTGGAGCCATCTGCCGGAAGAGTGTCAGGAAGATGCTGGAGGTGCTAGTGATGAAGCTGCCTCG
  CTCTGAGCACTGGGCCTTGCCTGGGGGCTCTAGGGAGCCAGGGGAGATGCTACCACGGAAGCTGAAACGG
  GTCCTCCGGCAGGAGTTCTGGGTGGCCTTTGAGACCTTGCTGATGCAAGGTACAGAGGTATACAAAGGGT
  ACGTGGATGACCCAAGGAACACAGACAATGCCTGGATCGAGACAGTGGCTGTCAGCATCCATTTTCAGGA
  CCAGAATGATATGGAGCTGAAGAGGCTGGAAGAGAACCTGCACACTCATGATCCAAAGGAGTTGACCCGT
  GACCTGAAGCTGTCTACTGAATGGCAGGTGGTAGACCGGCGGATCCCTCTGTATGCGAACCACAAGACCA
  TCCTCCAGAAGGTGGCCTCACTGTTTGGAGCTCACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225380 representing NM_138301
  Red=Cloning site Green=Tags(s)
  
  MESLDRRRTGSEQEEGFGVQSRRATDLGMVPNLRRSNSSLCKSRRFLCSFSSEKQENLSSWIPENIKKKE
  CVYFVESSKLSDAGKVVCACGYTHEQHLEVAIKPHTFQGKEWDPKKHVQEMPTDAFGDIVFTDLSQKVGK
  YVRVSQDTPSSVIYQLMTQHWGLDVPNLLISVTGGAKNFNMKLRLKSIFRRGLVKVAQTTGAWIITGGSH
  TGVMKQVGEAVRDFSLSSSCKEGEVITIGVATWGTIHNREGLIHPMGGFPAEYMLDEEGQGNLTCLDSNH
  SHFILVDDGTHGQYGVEIPLRTKLEKFISEQTKERGGVAIKIPIVCVVLEGGPGTLHTIYNAINNGTPCV
  IVEGSGRVADVIAQVATLPVSEITISLIQQKLSIFFQEMFETFTENQIVEWTKKIQDIVRRRQLLTIFRE
  GKDGQQDVDVAILQALLKASRSQDHFGHENWDHQLKLAVAWNRVDIARSEIFTDEWQWKPADLHPMMTAA
  LISNKPEFVRLFLENGVRLKEFVTWDTLLCLYENLEPSCLFHSKLQKVLAEEQRLAYASATPRLHMHHVA
  QVLRELLGDSTQLLYPRPRYTDRPRLSMTVPHIKLNVQGVSLRSLYKRSTGHVTFTIDPVRDLLIWAVIQ
  NHRELAGIIWAQSQDCTAAALACSKILKELSKEEEDTDSSEEMLALADEFEHRAIGVFTECYRKDEERAQ
  KLLVRVSEAWGKTTCLQLALEAKDMKFVSHGGIQAFLTKVWWGQLCVDNGLWRIILCMLAFPLLFTGFIS
  FREKRLQALCRPARVRAFFNAPVVIFHMNILSYFAFLCLFAYVLMVDFQPSPSWCEYLIYLWLFSLVCEE
  TRQLFYDPDGCGLMKMASLYFSDFWNKLDVGAILLFIVGLTCRLIPATLYPGRIILSLDFIMFCLRLMHI
  FTISKTLGPKIIIVKRMMKDVFFFLFLLAVWVVSFGVAKQAILIHNESRVDWIFRGVVYHSYLTIFGQIP
  TYIDGVNFSMDQCSPNGTDPYKPKCPESDWTGQAPAFPEWLTVTLLCLYLLFANILLLNLLIAMFNYTFQ
  EVQEHTDQIWKFQRHDLIEEYHGRPPAPPPLILLSHLQLLIKRIVLKIPAKRHKQLKNKLEKNEETALLS
  WELYLKENYLQNQQYQQKQRPEQKIQDISEKVDTMVDLLDMDQVKRSGSTEQRLASLEEQVTQVTRALHW
  IVTTLKDSGFGSGAGALTLAPQRAFDEPDAELSIRRKVEEPGDGYHVSARHLLYPNARIMRFPVPNEKVP
  WAAEFLIYDPPFYTAEKDVALTDPVGDTAEPLSKISYNVVDGPTDRRSFHGVYVVEYGFPLNPMGRTGLR
  GRGSLSWFGPNHTLQPVVTRWKRNQGGAICRKSVRKMLEVLVMKLPRSEHWALPGGSREPGEMLPRKLKR
  VLRQEFWVAFETLLMQGTEVYKGYVDDPRNTDNAWIETVAVSIHFQDQNDMELKRLEENLHTHDPKELTR
  DLKLSTEWQVVDRRIPLYANHKTILQKVASLFGAHF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138301
• ORF Size: 4518 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138301.2, NP_612174.2
• RefSeq Size: 7292 bp
• RefSeq ORF: 4521 bp
• Locus ID: 28240
• UniProt ID: Q91YD4
• Cytogenetics: 10 39.72 cM
• Gene Summary: Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels (PubMed:11804595, PubMed:19454650, PubMed:21753080, PubMed:22493272). Functions as ligand-gated ion channel. Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change; the channel is primed but still requires Ca(2+) binding to trigger channel opening. Extracellular calcium passes through the channel and increases channel activity (By similarity). Also contributes to Ca(2+) release from intracellular stores in response to ADP-ribose (PubMed:21753080). Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (PubMed:21753080). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels (By similarity). Activated by moderate heat (35 to 40 degrees Celsius) (PubMed:27533035, PubMed:27562954). Activated by intracellular ADP-ribose, beta-NAD (NAD(+)) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species (PubMed:19454650, PubMed:21753080, PubMed:22493272). The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius) (By similarity). Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius) (PubMed:22493272, PubMed:25817999). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis (PubMed:27533035, PubMed:27562954). Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels (PubMed:20921208, PubMed:25817999). Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection (PubMed:21709234). Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro) (PubMed:22493272). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels (PubMed:21753080). Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its function in increasing cytoplasmic Ca(2+) and Zn(2+) levels (By similarity). Confers susceptibility to cell death following oxidative stress (PubMed:25562606).[UniProtKB/Swiss-Prot Function]