Pdcd6ip (NM_001164677) Mouse Tagged ORF Clone

CAT#: MG225366

Pdcd6ip (tGFP-tagged) - Mouse programmed cell death 6 interacting protein (Pdcd6ip) transcript variant 1, (10ug)

Product Images

Circular map for MG225366

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pdcd6ip
• Synonyms: AI480591; Aip1; Alix; AW544830; C76364; Eig2; mKIAA1375
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225366 representing NM_001164677
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGTTCATCTGGGTGCAGCTGAAGAAGACCTCGGAGGTGGACCTGGCCAAGCCGCTGGTGAAGT
  TCATCCAGCAGACGTACCCGAGCGGCGGCGAGGAGCAGGCCCAGTACTGCCGTGCGGCCGAGGAGCTCAG
  CAAACTGCGGCGCTCGGCGCTCGGTCGCCCGCTGGACAAGCATGAGGGCGCCCTGGAGACGTTGCTGAGG
  TATTATGATCAGATTTGTTCCATTGAACCCAAGTTCCCATTTTCTGAAAATCAGATCTGCTTGACGTTCA
  CGTGGAAGGATGCTTTTGATAAAGGTTCCCTTTTTGGAGGGTCTGTAAAATTGGCTCTTGCAAGCTTAGG
  ATATGAAAAGAGCTGTGTGTTGTTCAATTGTGCTGCCTTAGCTAGCCAGATTGCAGCAGAGCAGAACCTG
  GATAATGATGAAGGATTGAAAACCGCTGCTAAGCAGTACCAGTTTGCTAGTGGTGCCTTTTTACATATTA
  AAGACACAGTGTTATCTGCCTTAAGTCGAGAGCCTACTGTGGACATATCTCCAGATACTGTTGGAACTCT
  CAGTCTTATTATGCTGGCTCAAGCTCAAGAAGTATTTTTCTTAAAAGCCACAAGAGATAAGATGAAAGAT
  GCCATCATAGCTAAGCTGGCAAATCAGGCTGCGGATTACTTTGGCGATGCTTTCAAGCAGTGTCAGTACA
  AGGACACGCTCCCCAAGTATTTTTATTTCCAGGAGGTATTCCCCACCCTGGCTGCAAAGCAGTGCATCAT
  GCAGGCCAATGCTGAGTACCACCAGTCCATCCTGGCCAAGCAGCAGAAGAAGTTTGGGGAAGAGATCGCA
  AGGTTGCAGCACGCAGCAGAACTGATCAAGAATGTGGCCTCTCGCTATGATGAGTATGTCAATGTGAAGG
  ATTTTTCTGACAAAATCAACCGTGCCCTTACTGCAGCAAAGAAGGATAATGATTTTATTTATCATGACCG
  TGTTCCGGACCTTAAGGATCTGGATCCTATCGGCAAAGCCACACTTGTGAAGCCCACCCCAGTCAATGTA
  CCTGTCAGCCAGAAGTTCACGGATTTGTTTGAGAAGATGGTCCCTGTGTCTGTGCAGCAGTCCCTGGCTG
  TGTTTAGTCAGAGGAAAGCTGACTTGGTCAACAGATCAATCGCTCAGATGAGAGAAGCTACGACTTTGGC
  AAATGGAGTATTGGCTTCCCTTAACCTTCCAGCAGCAATTGAAGATGTGTCTGGAGACACTGTACCTCAG
  TCTATACTTACCAAGTCTACATCTGTAGTTGAACAGGGAGGCATCCAGACTGTCGACCAGCTGATAAAAG
  AGCTACCTGAGCTGCTGCAAAGAAATAGAGAAATATTAGAGGAGTCGCTAAGATTGTTGGATGAAGAAGA
  AGCAACTGACAATGATTTAAGAGCAAAATTCAAGGACCGCTGGCAAAGGACTCCATCCAATGACCTGTAC
  AAGCCTTTACGAGCAGAGGGAGCCAAATTCAGAGCCGTTTTAGATAAAGCTGTGCAAGCGGATGGACAGG
  TGAAGGAGCGCTACCAGTCCCATCGAGACACCATCGCACTTCTGTGTAAGCCGGAGCCAGAGCTGAATGC
  TGCCATCCCCTCTGCTAACCCAGCAAAGACCATGCAGGGCAGCGAGGTTGTAAGTGTCTTAAAGTCCTTA
  TTATCAAATCTTGATGAAATCAAGAAGGAAAGAGAGAGTCTTGAGAATGACCTGAAGTCAGTGAATTTTG
  ACATGACAAGCAAGTTTTTGACAGCTCTGGCCCAAGATGGCGTGATAAATGAGGAGGCTCTCTCTGTCAC
  TGAGCTGGATCGGATCTATGGCGGTCTAACAAGTAAAGTTCAAGAGTCTCTGAAGAAACAAGAGGGACTT
  CTAAAAAATATACAGGTCTCACACCAAGAATTCTCCAAAATGAAGCAATCTAACAACGAGGCTAACTTGA
  GAGAAGAAGTTCTGAAGAACCTAGCAACTGCGTATGACAACTTTGTTGAGCTTGTAGCTAACTTGAAGGA
  GGGCACAAAGTTTTACAATGAGCTGACTGAGATCCTGGTCAGGTTCCAGAACAAATGCAGTGACATAGTG
  TTTGCACGGAAGACAGAAAGAGACGAGCTCTTGAAGGATCTGCAGCAGAGCATTGCCAGAGAGCCCAGCG
  CTCCTTCAATCCCTCCTCCAGCCTATCAGTCCTCCCCAGCAGCGGGGCATGCAGCAGCGCCTCCAACTCC
  AGCCCCAAGAACCATGCCGCCTGCTAAGCCCCAGCCTCCAGCCCGGCCTCCACCTCCTGTGCTTCCTGCA
  AACCGAGTTCCTCCTGCTTCTGCTGCTGCTGCCCCTGCAGGCGTGGGGACGGCTTCAGCAGCGCCGCCAC
  AGACCCCTGGCTCTGCTCCCCCGCCACAGGCTCAGGGACCACCATACCCTACCTATCCAGGATATCCCGG
  GTATTGCCAAATGCCCATGCCCATGGGCTACAACCCCTACGCATATGGCCAGTACAATATGCCGTACCCA
  CCGGTGTATCACCAGAGCCCCGGACAGGCTCCATACCCAGGACCCCAGCAGCCTACCTACCCCTTCCCTC
  AGCCCCCGCAGCAGTCCTACTATCCACAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225366 representing NM_001164677
  Red=Cloning site Green=Tags(s)
  
  MASFIWVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRSALGRPLDKHEGALETLLR
  YYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNL
  DNDEGLKTAAKQYQFASGAFLHIKDTVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKD
  AIIAKLANQAADYFGDAFKQCQYKDTLPKYFYFQEVFPTLAAKQCIMQANAEYHQSILAKQQKKFGEEIA
  RLQHAAELIKNVASRYDEYVNVKDFSDKINRALTAAKKDNDFIYHDRVPDLKDLDPIGKATLVKPTPVNV
  PVSQKFTDLFEKMVPVSVQQSLAVFSQRKADLVNRSIAQMREATTLANGVLASLNLPAAIEDVSGDTVPQ
  SILTKSTSVVEQGGIQTVDQLIKELPELLQRNREILEESLRLLDEEEATDNDLRAKFKDRWQRTPSNDLY
  KPLRAEGAKFRAVLDKAVQADGQVKERYQSHRDTIALLCKPEPELNAAIPSANPAKTMQGSEVVSVLKSL
  LSNLDEIKKERESLENDLKSVNFDMTSKFLTALAQDGVINEEALSVTELDRIYGGLTSKVQESLKKQEGL
  LKNIQVSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIV
  FARKTERDELLKDLQQSIAREPSAPSIPPPAYQSSPAAGHAAAPPTPAPRTMPPAKPQPPARPPPPVLPA
  NRVPPASAAAAPAGVGTASAAPPQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYP
  PVYHQSPGQAPYPGPQQPTYPFPQPPQQSYYPQQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164677
• ORF Size: 2622 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164677.1, NP_001158149.1
• RefSeq Size: 5976 bp
• RefSeq ORF: 2625 bp
• Locus ID: 18571
• UniProt ID: Q9WU78
• Cytogenetics: 9 F3
• Gene Summary: Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (By similarity). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (PubMed:27336173).[UniProtKB/Swiss-Prot Function]