Galns (NM_001193645) Mouse Tagged ORF Clone

CAT#: MG225100

Galns (tGFP-tagged) - Mouse galactosamine (N-acetyl)-6-sulfate sulfatase (Galns) transcript variant 2, (10ug)

Product Images

Circular map for MG225100

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Galns
• Synonyms: mFLJ00319
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225100 representing NM_001193645
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGGCGCGCCC
  
  ATGGCGGCCTGTACCGCAGCCCAGCAGCTGCTGCTGGTACTGAGTGCCCTAGGACTCTTGGCCGCAGGTG
  CTCCGCAGCCCCCCAACATCGTGCTACTGCTCATGGACGATATGGGGTGGGGTGACTTGGGTGTGAATGG
  AGAACCTTCCAGAGAGACCCCAAATTTAGACCGGATGGCTGCAGAAGGGATGCTTTTCCCAAGCTTCTAT
  TCTGCCAACCCTTTGTGCTCACCATCTAGGGCAGCCCTGCTCACAGGACGGCTGCCCATCCGCAATGGCT
  TCTACACAACCAACGCTCATGCAAGGAATGCTTACACACCTCAGGAGATCATGGGTGGGATCCCTAACTC
  AGAGCACCTCCTGCCCGAGCTCCTGAAGAAGGCAGGCTATACCAACAAGATCGTGGGCAAGTGGCATCTG
  GGTCATAGGCCCCAGTTCCACCCCCTGAAGCATGGATTTGACGAGTGGTTTGGATCTCCCAACTGTCACT
  TTGGACCGTATGACAACAAGGCCAAGCCCAACATCCCTGTGTACAGGGACTGGGAGATGGTCGGCAGATT
  TTATGAAGAGTTCCCAATTAACCGGAAGACTGGGGAAGCTAACCTCACCCAGCTCTACACACAAGAAGCG
  CTGGACTTCATCCAGACACAACATGCAAGGCAGAGCCCCTTCTTCCTCTACTGGGCCATCGACGCCACTC
  ATGCACCAGTTTACGCCTCAAGACAGTTCTTGGGCACCAGCCTTCGAGGGCGGTATGGCGATGCTGTTCG
  GGAAATAGATGACAGTGTTGGGAAGATCTTGAGCCTTCTGCAGAACTTGGGCATCAGTAAGAACACATTT
  GTCTTCTTCACGTCTGATAATGGTGCGGCCCTCATTTCTGCTCCCAACGAAGGTGGCAGCAACGGTCCCT
  TCCTGTGTGGGAAGCAGACGACATTTGAAGGCGGGATGAGGGAGCCTGCGATCGCATGGTGGCCAGGACA
  CATTGCTGCAGGCCAGGGCACTGACTTCTGCCCCGGGCAGAACGTCTCAGGAGTTACAACCCACACCCAG
  GAAGAACACACGGAGCTACCCCTGATCTTCCACCTGGGACGGGACCCAGGGGAGAGATTCCCACTCAGCT
  TCCACAGTGACGAATACCAGGACGCCCTCAGCAGGACCACCCAGGTTGTCCAGGAGCACCAAAAGTCCTT
  GGTCCCCGGACAGCCCCAGCTCAATGTGTGTAACCAGGCTGTCATGAATTGGGCACCCCCAGGCTGTGAA
  AAACTAGGGAAGTGTCTGACACCTCCTGAGTCTGTCCCTGAGAAGTGTTTCTGGGCCCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225100 representing NM_001193645
  Red=Cloning site Green=Tags(s)
  
  MAACTAAQQLLLVLSALGLLAAGAPQPPNIVLLLMDDMGWGDLGVNGEPSRETPNLDRMAAEGMLFPSFY
  SANPLCSPSRAALLTGRLPIRNGFYTTNAHARNAYTPQEIMGGIPNSEHLLPELLKKAGYTNKIVGKWHL
  GHRPQFHPLKHGFDEWFGSPNCHFGPYDNKAKPNIPVYRDWEMVGRFYEEFPINRKTGEANLTQLYTQEA
  LDFIQTQHARQSPFFLYWAIDATHAPVYASRQFLGTSLRGRYGDAVREIDDSVGKILSLLQNLGISKNTF
  VFFTSDNGAALISAPNEGGSNGPFLCGKQTTFEGGMREPAIAWWPGHIAAGQGTDFCPGQNVSGVTTHTQ
  EEHTELPLIFHLGRDPGERFPLSFHSDEYQDALSRTTQVVQEHQKSLVPGQPQLNVCNQAVMNWAPPGCE
  KLGKCLTPPESVPEKCFWAH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193645
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001193645.1, NP_001180574.1
• RefSeq Size: 2353 bp
• RefSeq ORF: 1323 bp
• Locus ID: 50917
• UniProt ID: Q571E4
• Cytogenetics: 8 E1