Bod1 (NM_001024919) Mouse Tagged ORF Clone

CAT#: MG225078

Bod1 (tGFP-tagged) - Mouse biorientation of chromosomes in cell division 1 (Bod1), (10ug)

Product Images

Circular map for MG225078

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bod1
• Synonyms: 2310022M17Rik; BOD2.1; Fam44b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225078 representing NM_001024919
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGTGCGGGAGCAGGCGCGGCGGGCCAGGCTTCGGGACCGTCCGGGGGCTCGAGTGGGGCCG
  GCGGCCCTGTCAATCCCGCTTCGCTGCCTCCGGGAGACCCGCAGCTCATCGCTCTCATAGTGGAGCAGCT
  CAAGAGCCGTGGGCTGTTTGACAGCTTCCGCCGGGACTGCCTGGCCGACGTGGACACTAAGCCAGCTTAC
  CAGAACCTAAGGCAGAAGGTGGATAATTTCGTGTCAACACATCTGGACAAACAGGAATGGAATCCTGCAA
  TGAACAAGAACCAGTTGCGAAATGGGCTGAGGCAGAGCGTGGTTCAGTCAGGTATGTTGGAAGCAGGAGT
  GGACAGGATCATTTCCCAAGTGGTAGACCCCAAACTAAACCACATCTTCAGGCCACAGATAGAGAGAGCA
  ATCCATGAGTTCCTGGCTGCCCAGAAAAAAGAAGCTGTGCCGGCCCCGCCTCCAGAACCTGAAAGCCAGG
  ACCCTCCAGCACCATCCCAGGATGCTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225078 representing NM_001024919
  Red=Cloning site Green=Tags(s)
  
  MADGAGAGAAGQASGPSGGSSGAGGPVNPASLPPGDPQLIALIVEQLKSRGLFDSFRRDCLADVDTKPAY
  QNLRQKVDNFVSTHLDKQEWNPAMNKNQLRNGLRQSVVQSGMLEAGVDRIISQVVDPKLNHIFRPQIERA
  IHEFLAAQKKEAVPAPPPEPESQDPPAPSQDAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024919
• ORF Size: 519 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024919.1, NP_001020090.1
• RefSeq Size: 1408 bp
• RefSeq ORF: 522 bp
• Locus ID: 69556
• UniProt ID: Q5SQY2
• Cytogenetics: 11 18.55 cM
• Gene Summary: Required for proper chromosome biorientation through the detection or correction of syntelic attachments in mitotic spindles.[UniProtKB/Swiss-Prot Function]