Wasf2 (NM_153423) Mouse Tagged ORF Clone

CAT#: MG224990

Wasf2 (tGFP-tagged) - Mouse WAS protein family member 2 (Wasf2), (10ug)

Product Images

Circular map for MG224990

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wasf2
• Synonyms: AW742646; D4Ertd13e; WAVE2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224990 representing NM_153423
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTAGTAACCAGGAACATCGAGCCAAGGCACCTGTGCCGTCAGACGTTGCCTAGCGATACAAGCG
  AGCTGGAATGCAGGACCAACATCACCCTGGCAAATGTCATCCGACAGCTGGGCAGCCTGAGTAAGTATGC
  AGAGGACATTTTCGGAGAGATTTGTACTCAGGCAAGTGCCTTTGCCTCCCGAGTAAACTCCCTTGCTGAG
  CGGGTTGACCGAGTACAAGTTAAAGTCACTCAGCTGGATCCCAAGGAAGAAGAAGTGTCACTACAAGGAA
  TCAACACTCGGAAGGCCTTCAGAAGTTCTACCACCCAAGACCAGAAGCTCTTTGACAGGAACTCTCTCCC
  GGTGCCCGTCTTAGAGACCTATAACAGCTGTGACGCTCCTCCCCCTCTTAACAATCTCAGTCCTTACAGG
  GACGATGGAAAAGAGGCACTCAAATTCTACACCAACCCCTCATACTTCTTTGATCTTTGGAAGGAGAAGA
  TGCTGCAGGACACCAAGGATATCATGAAAGAGAAGAGAAAGCATAGGAAAGAAAAGAAAGATAATCCAAA
  TAGAGGGAATGTGAACCCACGTAAAATCAAGACACGCAAGGAAGAGTGGGAGAAGATGAAAATGGGACAA
  GAATTTGTAGAGTCCAAGGAGAGGCTGGGGCCTTCTGGGTATTCGTCCACCTTGGTGTACCAGAATGGCA
  GCATTGGCTCTGTTGAAAATGTGGATGCGGCCAGCTACCCACCACCACCACAGTCGGACTCTGCCTCCTC
  ACCTTCCCCTTCATTCTCTGAAGACAACTTGCCTCCCCCGCCAGCAGAATTCAGCTACCCTGCAGACAAC
  CAAAGAGGATCTGTCTTGGCTGGACCCAAAAGAACCAGCATGGTCAGCCCAAGCCATCCACCCCCAGCTC
  CTCCTCTGAGCTCTCCGCCAGGTCCCAAGCCTGGCTTCGCTCCTCCACCTGCCCCTCCACCTCCACCTCC
  CATGAGTGTGCCACCTCCACTACCATCGATGGGATTCGGGTCTCCTGGGACCCCTCCACCCCCATCACCT
  CCATCTTTCCCTCCTCACCCTGATTTTGCTGCTCCTCCACCACCTCCCCCACCACCAGCAGCTGACTACC
  CAATGCCACCACCTCCTTTGTCCCAACCGTCTGGAGGTGCTCCTCCGCCTCCTCCTCCCCCTCCCCCTCC
  AGGGCCCCCGCCTCTCCCCTTCAGCGGTGCTGATGGCCAGCCTGCTGCACCACCACCACCACCACCTTCT
  GAGGCCACCAAGCCCAAGTCTTCATTGCCTGCTGTGAGTGACGCGCGCAGTGACCTGCTTTCTGCCATTC
  GCCAAGGCTTTCAGCTGCGAAGGGTTGAAGAGCAACGGGAGCAAGAGAAGCGTGATGTGGTGGGCAATGA
  TGTGGCCACCATCCTGTCCCGTCGGATCGCTGTTGAGTACAGCGACTCGGAAGATGATTCTTCTGAATTT
  GATGAAGACGACTGGTCGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224990 representing NM_153423
  Red=Cloning site Green=Tags(s)
  
  MPLVTRNIEPRHLCRQTLPSDTSELECRTNITLANVIRQLGSLSKYAEDIFGEICTQASAFASRVNSLAE
  RVDRVQVKVTQLDPKEEEVSLQGINTRKAFRSSTTQDQKLFDRNSLPVPVLETYNSCDAPPPLNNLSPYR
  DDGKEALKFYTNPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQ
  EFVESKERLGPSGYSSTLVYQNGSIGSVENVDAASYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPADN
  QRGSVLAGPKRTSMVSPSHPPPAPPLSSPPGPKPGFAPPPAPPPPPPMSVPPPLPSMGFGSPGTPPPPSP
  PSFPPHPDFAAPPPPPPPPAADYPMPPPPLSQPSGGAPPPPPPPPPPGPPPLPFSGADGQPAAPPPPPPS
  EATKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEF
  DEDDWSD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153423
• ORF Size: 1491 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153423.6, NP_700472.1
• RefSeq Size: 4088 bp
• RefSeq ORF: 1494 bp
• Locus ID: 242687
• UniProt ID: Q8BH43
• Cytogenetics: 4 66.22 cM
• Gene Summary: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity).[UniProtKB/Swiss-Prot Function]