Clcn4 (NM_011334) Mouse Tagged ORF Clone

CAT#: MG224964

Clcn4 (tGFP-tagged) - Mouse chloride channel 4-2 (Clcn4-2), (10ug)

Product Images

Circular map for MG224964

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Clcn4
• Synonyms: clC-4; Clc4-2; Clcn4-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224964 representing NM_011334
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTCCTCGAGGAGCCCTTCCCTGACGTGGGGACCTACGAGGACTTCCACACCATAGACTGGCTGA
  GGGAAAAGTCCCGGGATACCGACAGACATAGGAAGATCACCAGCAAAAGTAAGGAGTCTATTTGGGAGTT
  CATCAAGAGCCTGCTGGACGCGTGGTCGGGATGGGTGGTGATGCTACTCATTGGGCTGCTGGCAGGTACC
  TTGGCTGGAGTCATCGATCTCGCTGTGGATTGGATGACGGACCTCAAGGAGGGGGTCTGTCTGTCCGCAT
  TCTGGTACAGCCATGAACAGTGCTGTTGGACCTCCAACGAGACCACTTTTGAGGACAGGGACAAGTGTCC
  CCTGTGGCAGAAGTGGTCAGAGCTTCTTCTGAGCCAGTCAGAGGGCGCCAGCGCTTACATTCTGAATTAC
  TTAATGTACATTCTATGGGCGTTGCTGTTTGCATTTCTGGCTGTCTCCCTGGTACGTGTGTTCGCACCGT
  ATGCCTGTGGCTCTGGCATACCCGAGATAAAGACTATTTTGAGTGGCTTTATCATCAGGGGATACTTGGG
  GAAATGGACTCTTCTAATCAAGACTGTCACCCTCGTGCTCGTCGTATCCTCTGGCCTGAGCCTTGGCAAA
  GAGGGCCCACTGGTGCATGTGGCATGTTGCTGTGGCAACTTCTTCAGCAGCCTTTTCTCCAAGTATAGCA
  AGAATGAAGGCAAGAGGCGTGAGGTGCTTTCAGCTGCAGCTGCTGCTGGTGTCTCTGTGGCCTTTGGTGC
  TCCGATAGGAGGTGTGCTCTTCAGTCTAGAGGAGGTCAGTTACTACTTTCCCTTGAAAACCTTGTGGAGG
  TCATTCTTTGCAGCCCTGGTGGCTGCCTTCACACTGCGCTCCATCAACCCCTTTGGAAATAGCCGCCTGG
  TTCTCTTTTATGTGGAGTATCATACACCCTGGTACATGGCTGAACTCTTCCCTTTCATCCTGCTTGGAGT
  CTTTGGGGGTTTATGGGGAACCCTCTTCACACGCTGCAACATTGCTTGGTGCAGGAGGCGTAAGACCACC
  AGGCTGGGCAGGTACCCAGTGTTGGAGGTTATTGCGGTGACAGCCGTCACCGCCATCGTGGCCTACCCCA
  ATCCCTACACTCGCCAGAGCACCAGTGAGCTCATCTCTGAGCTCTTCAACGATTGTGGGGCTCTCGAGTC
  TTCTCAGCTCTGTGACTACATCAACGACCCCAACATGACTCGGCCTGTGGATGACATTCCGG[AT]CCGG
  CCGGCTGGGGTTGGAGTTTACACAGCCATGTGGCAGCTGGCCTTGGCACTGATCTTCAAAATAGTCATTA
  CTATATTTACCTTTGGCATGAAGATTCCCTCAGGTCTCTTCATCCCCAGTATGGCTGTCGGAGCCATGGC
  AGGCCGGATGGTGGGAATCGGTGTGGAGCAGCTGGCCTACCATCACCATGACTGGATCATCTTCAGGAAC
  TGGTGCAGGCCTGGAGCGGACTGTGTCACACCAGGGCTTTATGCGATGGTGGGAGCTGCAGCCTGTCTAG
  GTGGGGTGACTAGGATGACAGTGTCTCTAGTGGTCATTATGTTTGAACTGACTGGAGGTCTGGAGTATAT
  TGTACCCCTGATGGCAGCTGCTGTCACCAGCAAGTGGGTGGCTGATGCCTTTGGGAAAGAAGGGATTTAT
  GAAGCCCACATCCATCTGAATGGGTACCCATTTCTTGATGTGAAGGATGAGTTCACCCACCGTACGCTGG
  CCACTGATGTGATGCGGCCCCGGAGGGGAGAACCGCCATTATCGGTACTAACCCAGGACAGCATGACTGT
  GGAGGACGTGGAGACTCTCATCAAGGAGACAGACTACAACGGCTTTCCTGTGCTCGTCTCCAGAGACTCG
  GAGCGTCTCATCGGGTTTGCCCAGAGGCGGGAGCTAATCTTGGCTATAAAAAATGCCAGGCAGAGGCAAG
  AGGGCATTGTGAGCAATTCCATCATGTACTTCACAGAGGAGCCTCCTGAGCTGCCTGCCAACAGCCCACA
  TCCACTGAAGCTGAGGCGCATTTTGAACCTGAGCCCTTTCACGGTCACAGATCACACCCCCATGGAGACG
  GTGGTGGACATTTTCCGGAAACTGGGGCTCCGACAATGCCTGGTGACACGGAGTGGGAGACTTCTTGGGA
  TCATCACAAAAAAGGATGTTCTGAGACACATGGCCCAGATGGCAAACCAGGACCCTGAATCCATCATGTT
  TAAT
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224964 representing NM_011334
  Red=Cloning site Green=Tags(s)
  
  MDFLEEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWVVMLLIGLLAGT
  LAGVIDLAVDWMTDLKEGVCLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLLSQSEGASAYILNY
  LMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGK
  EGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWR
  SFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTLFTRCNIAWCRRRKTT
  RLGRYPVLEVIAVTAVTAIVAYPNPYTRQSTSELISELFNDCGALESSQLCDYINDPNMTRPVDDIPXXR
  PAGVGVYTAMWQLALALIFKIVITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRN
  WCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIY
  EAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVLVSRDS
  ERLIGFAQRRELILAIKNARQRQEGIVSNSIMYFTEEPPELPANSPHPLKLRRILNLSPFTVTDHTPMET
  VVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011334
• ORF Size: 2244 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011334.3, NP_035464.3
• RefSeq Size: 4620 bp
• RefSeq ORF: 2244 bp
• Locus ID: 12727
• UniProt ID: Q61418
• Cytogenetics: 7 4.23 cM
• Gene Summary: Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons (By similarity).[UniProtKB/Swiss-Prot Function]