Nipal4 (NM_172524) Mouse Tagged ORF Clone

CAT#: MG224884

Nipal4 (tGFP-tagged) - Mouse NIPA-like domain containing 4 (Nipal4), (10ug)

Product Images

Circular map for MG224884

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nipal4
• Synonyms: 9530066K23Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224884 representing NM_172524
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCGGGTCGCCAACGCCAATGGCAGCTGCGAGAACGGCTCCATAGTCAGCCTCTACTGCTCCT
  CTCAGGAGGTCCTCTGTCAGATCGTGAGAGGCATCAGTCCTGAAGAGCCCTACAATGCCACCTTGATCAC
  CTGGCAAGAGAGGGTCAGGAAGAAGTATGGCTTCTACATCGGTGTGGGCCTCGCCTTCCTCTCCTGCTTC
  CTCATCGGCACCAGTGTCATCCTTAAGAAGAAAGGCCTCATTCGGCTTGTGGCCACAGGGGCCACAAGGG
  CAGTGAATGGAGGCTACGGCTACCTGAAGGACCCAATGTGGTGGGCTGGAATGGCTACCATGTCTGCTGG
  AGAAGTTGCCAACTTCGGGGCCTATGCATTTGCACCTGCGACGGTTGTCACACCGCTGGGAGCACTGAGC
  GTCCTCATAAGTGCCATCTTTTCCTCCTACTGCCTGGGAGAGAGCCTGAATCTACTGGGGAAACTGGGCT
  GTGTGATCTGTATGGCTGGCAGCACAGTGATGGTCATACATGCCCCCAAAGAGGAGAAGGTCACCACTGT
  TGCAGAGATGGCTTCCAAGATGAAAGACACAGGGTTCATCGTCTTTGCTGTCCTTCTGGTGGTGTCTTGC
  CTCATCCTCATCTTCATTGTTGCCCCACGTTACGGGCAAAGGAATATCCTCATTTACATCATCATCTGCT
  CCGTGATCGGATCCTTCTCCGTGACTGCCGTCAAGGGGCTTGGTGTCACCATTAGGAACTTCTTCCAGGG
  CTTACCAGTTGTGCGGCACCCTCTCCCTTACATCCTGTCCCTCATCCTGGGTCTTTCTATCATCATCCAG
  GTCAACTTCCTCAACAGGGCACTGGATATTTTCAACACCTCATTGGTCTTCCCCATCTACTACGTTTTCT
  TCACCACGGTGGTGGTGGCCTCCTCCATCGTCCTCTTCAAGGAGTGGTATACCATGTCTGCTGTGGACAT
  TGTGGGCACCCTGTCTGGCTTCGTCACCATCATCCTGGGTGTTTTTATGCTTCACGCTTTCAAGGATTTG
  GACATCAACCAAATCAGCTTGCCCCACACGCACAAAAACCCAACTCCAGCTCCCGCCCCAGAGCCCACAG
  TCATTAAACTAGAAGACAAGAATGTCCTTGTAGATAACATAGAACTTGCCAGCACACCATCACCCCAACA
  GAAGCCCAAAGTATTTATGACCGATTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224884 representing NM_172524
  Red=Cloning site Green=Tags(s)
  
  MELRVANANGSCENGSIVSLYCSSQEVLCQIVRGISPEEPYNATLITWQERVRKKYGFYIGVGLAFLSCF
  LIGTSVILKKKGLIRLVATGATRAVNGGYGYLKDPMWWAGMATMSAGEVANFGAYAFAPATVVTPLGALS
  VLISAIFSSYCLGESLNLLGKLGCVICMAGSTVMVIHAPKEEKVTTVAEMASKMKDTGFIVFAVLLVVSC
  LILIFIVAPRYGQRNILIYIIICSVIGSFSVTAVKGLGVTIRNFFQGLPVVRHPLPYILSLILGLSIIIQ
  VNFLNRALDIFNTSLVFPIYYVFFTTVVVASSIVLFKEWYTMSAVDIVGTLSGFVTIILGVFMLHAFKDL
  DINQISLPHTHKNPTPAPAPEPTVIKLEDKNVLVDNIELASTPSPQQKPKVFMTDS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172524
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172524.3, NP_766112.1
• RefSeq Size: 3295 bp
• RefSeq ORF: 1221 bp
• Locus ID: 214112
• UniProt ID: Q8BZF2
• Cytogenetics: 11 B1.1
• Gene Summary: Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Ba(2+), Mn(2+), Sr(2+) and Co(2+) but to a much less extent than Mg(2+). May be a receptor for ligands (trioxilins A3 and B3) from the hepoxilin pathway (By similarity).[UniProtKB/Swiss-Prot Function]