Pbrm1 (NM_001081251) Mouse Tagged ORF Clone

CAT#: MG224882

Pbrm1 (tGFP-tagged) - Mouse polybromo 1 (Pbrm1), (10ug)

Product Images

Circular map for MG224882

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pbrm1
• Synonyms: 2610016F04Rik; AI507524; BAF180; Pb1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224882 representing NM_001081251
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCCAAGAGAAGAAGAGCCACCTCTCCTTCCAGCAGTGTCAGTGGAGACTTTGATGACGGGCACC
  ATTCTGTGCCTACACCAGGCCCAAGCAGGAAAAGGAGAAGACTGTCCAATCTTCCAACTGTAGATCCTAT
  TGCTGTGTGCCATGAACTCTATAACACCATCCGAGACTATAAGGATGAACAGGGCAGACTCCTCTGTGAG
  CTGTTCATTAGGGCTCCAAAGCGGAGAAATCAACCAGACTATTATGAAGTGGTTTCTCAGCCCATTGACT
  TGATGAAAATCCAACAGAAACTTAAAATGGAAGAGTATGATGATGTTAATCTACTGACTGCTGACTTCCA
  GCTGCTTTTTAACAATGCAAAGGCCTACTATAAGCCAGATTCCCCTGAGTATAAAGCTGCTTGTAAACTC
  TGGGATTTGTACCTTCGAACAAGAAATGAGTTTGTTCAGAAAGGAGAAGCAGACGATGAAGATGATGACG
  AAGATGGGCAAGACAATCAAGGCACACTGGCTGACGGCTCTTCTCCAGGTTATCTGAAGGAGATCCTGGA
  GCAGCTTCTTGAAGCCATAGTTGTAGCCACAAATCCATCAGGACGGCTCATCAGTGAACTTTTTCAGAAA
  CTGCCTTCCAAAGTGCAATATCCAGACTATTATGCAATAATTAAGGAACCTATAGATCTCAAGACCATTG
  CTCAGAGGATACAGAATGGAAGCTACAAAAGTATACACGCAATGGCCAAAGATATAGATCTTCTAGCAAA
  AAATGCCAAAACATACAATGAGCCTGGGTCTCAAGTATTCAAGGATGCCAATTCGATTAAAAAAATATTT
  TATATGAAAAAGGCAGAAATTGAACATCATGAAATGACTAAATCAAGTCTTCGAATAAGGACTGCATCAA
  ATTTGGCTGCAGCCAGGCTGACAGGTCCTTCGCACAATAAAAGCAGCCTTGGTGAAGAAAGAAACCCCAC
  TAGCAAGTATTACCGTAATAAAAGAGCAGTCCAAGGGGGTCGCTTGTCAGCAATTACCATGGCACTTCAG
  TATGGATCAGAGAGTGAAGAGGACGCTGCTTTAGCTGCTGCACGCTATGAAGAAGGGGAATCTGAAGCAG
  AGAGCATCACTTCCTTCATGGACGTTTCCAACCCCTTTCATCAGCTTTACGACACAGTTAGGAGCTGTAG
  GAATCACCAAGGGCAGCTCATAGCTGAACCTTTCTTCCATTTGCCTTCAAAGAAAAAATACCCAGATTAT
  TATCAGCAAATTAAAATGCCCATATCACTTCAACAGATCAGAACAAAGCTAAAGAACCAAGAATATGAAA
  CTTTAGATCATTTGGAGTGTGATCTGAATTTAATGTTTGAAAATGCCAAACGTTATAACGTTCCCAATTC
  AGCCATCTATAAGCGAGTTCTAAAACTGCAGCAAGTCATGCAGGCAAAGAAGAAGGAGCTTGCGAGGAGA
  GATGACATTGAGGACGGAGACAGCATGATCTCCTCAGCCACTTCTGACACTGGTAGTGCCAAAAGGAAAA
  GGAATACTCATGACAGTGAGATGTTGGGTCTCAGGAGGCTATCCAGTAAAAAGAACATAAGAAAACAGCG
  AATGAAAATTTTATTCAATGTTGTTCTTGAAGCTCGAGAGCCAGGTTCAGGCAGAAGACTTTGCGATCTA
  TTTATGGTTAAGCCATCCAAGAAGGACTATCCTGATTATTATAAAATCATCTTAGAGCCAATGGACCTGA
  AAATAATTGAGCATAACATCCGAAATGACAAATATGCAGGTGAAGAAGGAATGATGGAAGACATGAAACT
  CATGTTCCGCAATGCCAGGCACTACAATGAGGAGGGCTCCCAGGTATACAATGATGCCCATATCCTGGAG
  AAGTTACTCAAAGATAAAAGGAAAGAGCTGGGCCCTCTGCCTGATGATGATGACATGGCTTCTCCCAAAC
  TTAAATTGAGTAGGAAGAGTGGTGTTTCTCCTAAGAAATCAAAGTACATGACTCCAATGCAGCAGAAACT
  GAATGAAGTGTATGAAGCTGTAAAGAACTATACTGATAAGAGGGGTCGCCGCCTTAGTGCTATATTTCTA
  AGACTCCCCTCTAGATCAGAGCTGCCTGACTACTACCTGACCATTAAAAAGCCCATGGACATGGAAAAAA
  TTCGAAGTCACATGATGGCAAACAAGTACCAAGACATAGATTCTATGGTAGAGGACTTTGTCATGATGTT
  TAATAATGCCTGTACCTACAATGAACCAGAGTCTTTGATCTACAAAGATGCCCTTGTACTGCATAAAGTC
  CTCCTTGAGACTCGGAGAGACCTGGAGGGAGATGAGGATTCTCATGTCCCTAATGTGACGTTGCTGATTC
  AAGAGCTCATCCATAACCTTTTTGTGTCAGTCATGAGTCATCAGGATGACGAAGGGAGGTGTTACAGCGA
  CTCCTTAGCAGAAATTCCTGCTGTGGATCCCAACTCTCCCAATAAACCTCCCCTTACATTTGACATTATC
  AGGAAAAATGTTGAAAGTAATCGGTATCGGCGACTTGATTTATTTCAGGAGCATATGTTTGAAGTATTGG
  AACGGGCAAGAAGGATGAACCGGACAGATTCCGAAATATATGAGGATGCTGTAGAACTTCAGCAGTTTTT
  TATTAGAATTCGTGATGAACTCTGCAAAAATGGAGAGATCCTTCTTTCTCCAGCACTCAGCTATACCACA
  AAACACTTGCATAACGATGTGGAAAAAGAAAAAAAGGAAAAATTGCCTAAAGAAATAGAGGAAGATAAAC
  TAAAACGCGAAGAAGAAAAAAGAGAAGCTGAAAAAAGTGAAGATTCCTCAGGTACTACAGGCCTCTCAGG
  CTTACATCGTACATACAGCCAGGACTGCAGCTTTAAGAACAGCATGTATCATGTCGGAGATTATGTCTAT
  GTTGAACCTGCGGAGGCCAATCTACAACCACATATAGTGTGTATTGAGAGACTGTGGGAGGATTCAGCTG
  GTGAAAAATGGTTGTACGGCTGTTGGTTTTATCGGCCAAATGAAACATTCCATTTGGCTACACGAAAATT
  TCTAGAAAAAGAAGTTTTTAAGAGTGACTACTACAATAAAGTACCTGTTAGTAAAATTCTAGGCAAATGT
  GTAGTCATGTTTGTCAAGGAATACTTTAAATTATGTCCAGAAAACTTTCGCGATGAGGATGTTTTTGTCT
  GTGAATCGAGGTATTCTGCCAAAACCAAATCTTTTAAGAAAATTAAACTGTGGACCATGCCCATCAGTTC
  AGTTAGATTTGTCCCTCGGGATGTGCCTTTGCCTGTGGTCCGAGTGGCCTCTGTGTTTGCAAATGCAGAT
  AAAGGGGATGATGAGAAGAATACAGACAACTCAGATGACAATAGAGCTGAAGACAATTTTAACTTGGAAA
  AGGAAAAAGAAGATGTTCCTGTGGAGATGTCCAATGGTGAGCCAGGTTGCCACTACTTTGAGCAGCTTCG
  GTACAATGACATGTGGCTGAAGGTTGGTGATTGTGTCTTCATCAAATCCCACGGCTTGGTGCGCCCTCGT
  GTGGGCAGAATTGAGAAAGTATGGGTCCGAGATGGAGCTGCATATTTTTATGGCCCTATCTTCATTCATC
  CAGAAGAAACAGAACATGAGCCCACAAAAATGTTCTACAAAAAAGAAGTGTTTCTGAGTAATCTGGAAGA
  GACCTGCCCTATGAGTTGTATTCTGGGGAAATGTGCAGTGCTGTCATTCAAGGACTTCCTCTCCTGCAGG
  CCAACTGAAATACCAGAAAATGACATTCTGCTTTGTGAGAGCCGCTATAATGAGAGTGACAAGCAGATGA
  AGAAGTTCAAGGGTTTGAAGAGGTTTTCACTCTCTGCTAAAGTTGTAGATGATGAAATCTACTACTTCAG
  AAAACCAATCATTCCTCAGAAGGAACCCTCACCTTTGTTAGAAAAGAAGATACAATTGCTAGAAGCTAAA
  TTTGCAGAGTTAGAAGGAGGAGATGATGATATTGAGGAGATGGGAGAAGAGGATAGTGAAGTCATTGAAG
  CTCCATCTCTACCTCAACTGCAGACACCCCTGGCCAATGAGTTGGACCTCATGCCCTATACACCCCCACA
  GTCTACCCCAAAGTCTGCCAAAGGCAGTGCAAAGAAGGAAAGTTCTAAACGAAAAATCAACATGAGTGGC
  TACATTTTGTTCAGCAGTGAAATGAGAGCTGTGATTAAAGCCCAGCACCCAGACTACTCTTTTGGGGAGC
  TCAGCAGACTGGTGGGGACAGAATGGAGAAACCTTGAAACAGCCAAGAAAGCAGAATATGAAGAGCGGGC
  AGCTAAAGTTGCTGAGCAGCAGGAGAGAGAGCGAGCAGCACAGCAACAGCAGCCGAGTGCTTCTCCCCGA
  GCAGGCACCCCTGTGGGGGCTCTCATGGGGGTGGTGCCACCACCAACACCAATGGGGATGCTCAATCAGC
  AGTTGACACCTGTTGCAGGCATGATGGGTGGCTATCCGCCAGGCCTTCCACCTTTGCAGGGCCCAGTTGA
  TGGCCTTGTTAGCATGGGCAGCATGCAGCCACTTCACCCTGGGGGGCCTCCACCTCACCATCTTCCGCCA
  GGTGTGCCTGGCCTCCCAGGCATCCCACCACCGGGTGTGATGAATCAAGGAGTAGCCCCCATGGTAGGGA
  CTCCAGCACCAGGTGGAAGTCCGTATGGACAACAGGTAGGAGTTTTGGGACCTCCAGGGCAGCAGGCACC
  ACCTCCATATCCTGGTCCTCATCCAGCTGGCCCCCCTGTCATACAGCAGCCAACAACGCCCATGTTTGTG
  GCTCCCCCACCAAAGACCCAAAGGCTTCTCCACTCAGAGGCCTACCTGAAATACATTGAAGGACTCAGTG
  CTGAATCCAACAGCATTAGCAAGTGGGACCAAACTTTGGCAGCTCGAAGACGGGATGTCCATTTGTCCAA
  AGAACAGGAGAGCCGCCTACCTTCTCACTGGCTCAAAAGTAAAGGGGCACACACCACCATGGCAGATGCC
  CTCTGGCGCCTACGGGATTTAATGCTTCGAGACACTCTCAACATCCGACAGGCATACAACCTAGAAAATG
  TT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224882 representing NM_001081251
  Red=Cloning site Green=Tags(s)
  
  MGSKRRRATSPSSSVSGDFDDGHHSVPTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCE
  LFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKL
  WDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTLADGSSPGYLKEILEQLLEAIVVATNPSGRLISELFQK
  LPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIF
  YMKKAEIEHHEMTKSSLRIRTASNLAAARLTGPSHNKSSLGEERNPTSKYYRNKRAVQGGRLSAITMALQ
  YGSESEEDAALAAARYEEGESEAESITSFMDVSNPFHQLYDTVRSCRNHQGQLIAEPFFHLPSKKKYPDY
  YQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARR
  DDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDL
  FMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMMEDMKLMFRNARHYNEEGSQVYNDAHILE
  KLLKDKRKELGPLPDDDDMASPKLKLSRKSGVSPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFL
  RLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKV
  LLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNSPNKPPLTFDII
  RKNVESNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIRIRDELCKNGEILLSPALSYTT
  KHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGTTGLSGLHRTYSQDCSFKNSMYHVGDYVY
  VEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKC
  VVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANAD
  KGDDEKNTDNSDDNRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLRYNDMWLKVGDCVFIKSHGLVRPR
  VGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMSCILGKCAVLSFKDFLSCR
  PTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAK
  FAELEGGDDDIEEMGEEDSEVIEAPSLPQLQTPLANELDLMPYTPPQSTPKSAKGSAKKESSKRKINMSG
  YILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPR
  AGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPP
  GVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPAGPPVIQQPTTPMFV
  APPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADA
  LWRLRDLMLRDTLNIRQAYNLENV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081251
• ORF Size: 5112 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081251.1, NP_001074720.1
• RefSeq Size: 8117 bp
• RefSeq ORF: 5115 bp
• Locus ID: 66923
• Cytogenetics: 14 B