Kiz (NM_001033298) Mouse Tagged ORF Clone

CAT#: MG224872

Plk1s1 (tGFP-tagged) - Mouse polo-like kinase 1 substrate 1 (Plk1s1), (10ug)

Product Images

Circular map for MG224872

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kiz
• Synonyms: Gm114; Ncrna00153; Plk1s1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224872 representing NM_001033298
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCGAGGGCGCGGTGGCGGTGGTGGTGGCCTGCGCCAGGCCTCCGCCACCTCTGCGCCCTTGGCGA
  GCCCCAGCTACTACGAAAGGGTGGGCCAGCTCCAGCAGGCTCTGCGGGACAGTGAAAAGAAGAGATTGGA
  CCTGGAAGACAAGCTTTATGAATACAATAAATCTGATAAGTGCAGAGCTAAGCTGAAATGTGCAAAACTA
  AAGAAATACCTGAAGGAAGTATGTGAGTCTGAGAGGAGGGCTCAGGTTCGAAACCAAGGATATTTAAAGA
  AGTTTGAATGTGTCCAGACGTATGTTGAACACTTGACCACAAATACAGAGAAGCTTCAAAAACTGAAGAC
  TGAATATGAGGCTGAAGTGAAGAGGATGCGCCTCCTGTCAAAGGATAGCCTGGGAATGAGTGACGAAGAC
  GGAGCAAAGGATGCAGTGCAGGCAGGAATTAACTCGGGGACAGCTATGTCAAGAGGATTGTATCAACCAG
  CAACAATCTTTATGGGCCGCCAAATGTCAGCCATCTCAAGCATTGAAGATTTCAGTACAGAGCTGAAATC
  TAACCAACCCACAAAGAACTTTTCAATCTCTGACCCACATTCACACCAACAGACAGCCCAGAGCAGCTGT
  GTGACAGACAGCTGTGTAGTGCAGACTAATGGTGACACACAGTGCTTAAATAAGTCTGACAAAATACATG
  GAAAGACATCTCTTCAGACTGGTGAGAAGGCGCCAGTCACAAGCTATGTATTGTCTGCAGAAGAACAAAC
  TCATTGCTTGGAGATAGGAAGCAGCACACAGCACAGCAAGAGTAATTTATCTGAAGGCAGAAAGTCTGCT
  GAACTCCATTCCTCGTTACAGGAAAGATTAAGTCCAGAGAACAGCATCACTGATTTAAAGTGTGACAGTT
  CCAGCAGATCAGAGGGATCAGACAGAGAAATACTGACACAAGAACATATTGAAGTCAGGGAAGAAAGAGC
  CGGCCCGCTGGTCCCCATGATGGCAGCTTCAGAACACTGTACATCTGCAAAGAAGTGGGCTGGAGAGAAG
  CATTCTGCTTGGGAAGCTTCCTCAGATGATCTTGACCACGGGGACTCAAAGTCACAGAAGGCCGTCCTGA
  AACATGAGGAAGAGCAGGAGGAGGGAAGCTCATGCAGCAGCAGCGACCTCACAGTTTCTGTCAGTGAAGA
  TGATCTGATTCTAGAGAGTCTGGCCGCACTGTCGAATCCAGGAGCCAAGATGGCGGGCAAAGATGGAATG
  CAGGCCTTGCGAGCGGCCCACACTGAACCAAGACAAGATTCGCTCTTCACGGAATATGTTTTACAGACCC
  AAAGCTTTCCTGATTCAAAAAGGGAACCCTCCCCAGATTCACCAAGACAACCTGAAAAAGTACCAGATTG
  TCACTTATTGAAGACACGAGGGCAATGTATGAAGGAACATGATAATTCTCTTAAAGAGGAGGCAACAACA
  TTATTGAAAAAAGTCCTTACAGAAGAATGTGACCATAGGTCAGCTATTCACAGTAATGAATCATCTTGCA
  GCATGCCATCTATTCTGAATGACAATAATGGAATAAAGGAAGCAAAACCCGCTCTGCGGCTCAACAGTGT
  TCTTACAAGGGAACAAGAAGTTTCAAGTGGCTGTGGAGATGAGAGCAAGGAAGAAAGCATAGCTGCGGCC
  CCAGGCACAGGACACAGAGAGGCGGATCTAGTACCCTCAGAACTGTTTCAGTTCAGCTTGGGAGCAGCCA
  TGAAAGAAACAAAAGCCTACCAGTTACTGAAGAAGTCTACCCTTCAGGACAATTCAAATCAAGCTGAAGA
  GAGATTTGAAAGTCAGTTTTCAGGTCTGGATATTGGTGGCAGCATGTTCACAACAAAGACAGCCCACAAA
  ATCGCCTCGGAAGCTAGTTTTTCATCTAGTGAAGGAAGTCCTTTGTCAAGGCACGAAAGCAAAAGGGACC
  CAGTGACCACCATAAAGTCAAATGCCTTCTGGGGGGAGTCTGATGACAGTAACTCTGAAATCGAAGCTGC
  CCTACGTCCTAGAGACCATGACATGCCCGATGATTTTGATGATTTTTATGACACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224872 representing NM_001033298
  Red=Cloning site Green=Tags(s)
  
  MPRGRGGGGGGLRQASATSAPLASPSYYERVGQLQQALRDSEKKRLDLEDKLYEYNKSDKCRAKLKCAKL
  KKYLKEVCESERRAQVRNQGYLKKFECVQTYVEHLTTNTEKLQKLKTEYEAEVKRMRLLSKDSLGMSDED
  GAKDAVQAGINSGTAMSRGLYQPATIFMGRQMSAISSIEDFSTELKSNQPTKNFSISDPHSHQQTAQSSC
  VTDSCVVQTNGDTQCLNKSDKIHGKTSLQTGEKAPVTSYVLSAEEQTHCLEIGSSTQHSKSNLSEGRKSA
  ELHSSLQERLSPENSITDLKCDSSSRSEGSDREILTQEHIEVREERAGPLVPMMAASEHCTSAKKWAGEK
  HSAWEASSDDLDHGDSKSQKAVLKHEEEQEEGSSCSSSDLTVSVSEDDLILESLAALSNPGAKMAGKDGM
  QALRAAHTEPRQDSLFTEYVLQTQSFPDSKREPSPDSPRQPEKVPDCHLLKTRGQCMKEHDNSLKEEATT
  LLKKVLTEECDHRSAIHSNESSCSMPSILNDNNGIKEAKPALRLNSVLTREQEVSSGCGDESKEESIAAA
  PGTGHREADLVPSELFQFSLGAAMKETKAYQLLKKSTLQDNSNQAEERFESQFSGLDIGGSMFTTKTAHK
  IASEASFSSSEGSPLSRHESKRDPVTTIKSNAFWGESDDSNSEIEAALRPRDHDMPDDFDDFYDT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033298
• ORF Size: 2085 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033298.4
• RefSeq Size: 2150 bp
• RefSeq ORF: 2088 bp
• Locus ID: 228730
• UniProt ID: Q3UXL4
• Cytogenetics: 2 G2
• Gene Summary: Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.[UniProtKB/Swiss-Prot Function]